%0 Journal Article %A Negredo, A. %A Monteoliva, L. %A Gil, C. %A Pla, J. %A Nombela, C. %T Cloning, analysis and one-step disruption of the ARG5,6 gene of Candida albicans %D 1997 %J Microbiology, %V 143 %N 2 %P 297-302 %@ 1465-2080 %R https://doi.org/10.1099/00221287-143-2-297 %K molecular biology %K gene disruption %K arginine %K ARG5,6, Candida albicans %I Microbiology Society, %X The ARG5,6 gene from the dimorphic fungus Candida albicans was cloned by functional complementation of the arginine auxotrophy present in strain EL2 (Arg-) using a gene library constructed in the double autonomously replicating sequence vector pRM1. Sequence analysis revealed a putative 857 amino acid polypeptide (95 kDa) which showed high homology (63% protein identity) to the Saccharomyces cerevisiae ARG5,6 gene. Similarly to the S. cerevisiae gene, the C. albicans ARG5,6 gene is responsible for both the acetylglutamate kinase and acetylglutamyl-phosphate reductase activities, the second and third steps of arginine biosynthesis at the mitochondria. The C. albicans ARG5,6 gene complemented the arg6 mutation present in S. cerevisiae (strain D160-4D) on a yeast episomal plasmid using its own regulatory signals. A set of non-integrative high-efficiency plasmid vectors based on this gene marker was constructed and a null C. albicans arg5,6d strain was obtained using the common URA3-blaster strategy. In addition, we generated an arg5,6d null mutant in a single transformation event, thus improving the basic strategy for generating gene deletions in C. albicans. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-2-297