1887

Abstract

The effect of the polycation polyethyleneimine (PEI) on the permeability properties of the Gram-negative bacterial outer membrane was investigated using and as target organisms. At concentrations of less than 20 µg ml, PEI increased the bacterial uptake of 1--phenylnaphthylamine, which is a hydrophobic probe whose quantum yield is greatly increased in a lipid environment, indicating increased hydrophobic permeation of the outer membrane by PEI. The effect of PEI was comparable to that brought about by the well-known permeabilizer EDTA. Permeabilization by PEI was retarded but not completely inhibited by millimolar concentrations of MgCl. PEI also increased the susceptibility of the test species to the hydrophobic antibiotics clindamycin, erythromycin, fucidin, novobiocin and rifampicin, without being directly bactericidal. PEI sensitized the bacteria to the lytic action of the detergent SDS in assays where the bacteria were pretreated with PEI. In assays where PEI and SDS were simultaneously present, no sensitization was observed, indicating that PEI and SDS were inactivating each other. In addition, a sensitizing effect to the nonionic detergent Triton X-100 was observed for In conclusion, PEI was shown to be a potent permeabilizer of the outer membrane of Gram-negative bacteria.

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1997-10-01
2021-05-15
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References

  1. Bahulekar R., Ayyangar N. R., Ponrathnam S. 1991; Polyethyleneimine in immobilization of biocatalysts. Enzyme Microb Technol 13:858–868
    [Google Scholar]
  2. Cong L., Kaul R., Dissing U., Mattiasson B. 1995; A model study on eudragit and polyethyleneimine as soluble carriers of α- amylase for repeated hydrolysis of starch. J Biotechnol 42:75–84
    [Google Scholar]
  3. Cordes R. M., Sims W. B., Glatz C. E. 1990; Precipitation of nucleic acids with poly(Ethyleneimine). Biotechnol Prog 6:283–285
    [Google Scholar]
  4. Elferink J. G. R. 1991; Changes of plasma membrane permeability in neutrophils treated with polycations. Inflammation 15:103–115
    [Google Scholar]
  5. Freer E., Moreno E., Moriydn I., Pizarro-Cerda J., Weintraub A., Gorvel J.-P. 1996; Brucella–salmonella. J Bacteriol 178:5867–5876
    [Google Scholar]
  6. Hancock R. E. W., Farmer S. W., Li Z., Poole K. 1991; Interaction of aminoglycosides with the outer membranes and purified lipopolysaccharide and ompf porin of. Antimicrob Agents Chemother 351309–1314
    [Google Scholar]
  7. Helander I. M., Makela P. H., Westphal O., Rietschel E. T. 1996; Lipopolysaccharides. In Encyclopedia of Molecular Biology and Molecular Medicine vol. 3 pp. 462–471 Edited by Meyers R. A. Weinheim: VCH;
    [Google Scholar]
  8. Hitchcock P. J., Brown T. M. 1983; Morphological heterogeneity among. J Bacteriol 154:269–277
    [Google Scholar]
  9. Kirk N., Cowan D. 1995; Optimising the recovery of recombinant thermostable proteins expressed in mesophilic hosts. J Biotechnol 42:177–184
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature 227:680–685
    [Google Scholar]
  11. Leive L. 1965; Release of lipopolysaccharide by edta treatment of. Biochem Biophys Res Commun 21:290–296
    [Google Scholar]
  12. Loh B., Grant C., Hancock R. E. W. 1984; Use of the fluorescent probe 1-n-phenylnaphthylamine to study the interactions of aminoglycoside antibiotics with the outer membrane of. Antimicrob Agents Chemother 26:546–551
    [Google Scholar]
  13. Milburn P., Bonnerjea J., Hoare M., Dunnill P. 1990; Selective flocculation of nucleic acids, lipids, and colloidal particles from a yeast cell homogenate by polyethyleneimine, and its scale-up. Enzyme Microb Technol 12:527–532
    [Google Scholar]
  14. Moore L. V. H., Bourne D. M., Moore W. E. C. 1994; Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacterial 44:338–347
    [Google Scholar]
  15. Nikaido H. 1989; Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob Agents Chemother 33:1831–1836
    [Google Scholar]
  16. Salt D. E., Hay S., Thomas O., Hoare M., Dunnill P. 1995; Selective flocculation of cellular contaminants from soluble proteins using polyethyleneimine: A study of several organisms and polymer molecular weights. Enzyme Microb Technol 17:107–113
    [Google Scholar]
  17. Skyttck E., Mattila-Sandholm T. 1991; A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry. J Microbiol Methods 14:77–88
    [Google Scholar]
  18. Suh J., Cho Y., Lee K. J. 1991; Macrocyclic metal complexes built on polyethyleneimine. J am Chem Soc 113:4198–4202
    [Google Scholar]
  19. Tashiro T. 1992; Removal of bacteria from water by systems based on insoluble polystyrene-polyethylenimine. J Appl Polym Sci 46:899–907
    [Google Scholar]
  20. Trouble H., Overath P. 1973; The structure of. Biochim Biophys Acta 307:491–512
    [Google Scholar]
  21. Tsuchido T., Aoki I., Takano M. 1989; Interaction of the fluorescent dye 1-n-phenylnaphthylamine with. J Gen Microbiol 135:1941–1947
    [Google Scholar]
  22. Vaara M. 1992; Agents that increase the permeability of the outer membrane. Microbiol Rev 56:395–411
    [Google Scholar]
  23. Vaara M., Vaara T. 1983; Polycations sensitize enteric bacteria to antibiotics. Antimicrob Agents Chemother 24:107–113
    [Google Scholar]
  24. Vaara M., Vaara T. 1983; Polycations as outer membrane- disorganizing agents. Antimicrob Agents Chemother 24:114–122
    [Google Scholar]
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