1887

Abstract

Bacterial heat-shock proteins (HSPs) from (GroES, GroEL and DnaK) were studied for their ability to induce by themselves the expression and release of tumour necrosis factor-a (TNF-a), interleukin-6 (IL-1a), interleukin-6 (IL-6) and intercellular adhesion molecule-1 (ICAM-1) by cultured human keratinocytes. The surface expression of ICAM-1 was also investigated. In the supernatants of untreated cells none or a minimal amount of these molecules was found. After 48 h of stimulation with GroEL significant amounts of TNF-a, IL-1a, IL-6 and soluble ICAM-1 were detected, reaching maximum concentrations at 1 g ml. The same effect was elicited by DnaK but to a lesser extent. Treatment of keratinocytes with GroEL and DnaK also increased TNF-a, IL-1a, IL-6 and ICAM-1 mRNA levels. GroES showed significant activity only on the expression and release of IL-6. GroEL and DnaK were also able to up-regulate the surface expression of ICAM-1 on keratinocytes. The effects on ICAM-1 expression seemed to be directly due to HSPs and not mediated via cytokines. Furthermore, these effects were due to the properties of HSPs because they were inhibited by specific monoclonal antibodies. These findings support the potential role of HSPs in modulating cell interactions during immunological and inflammatory responses in the skin.

Keyword(s): heat-shock proteins , ICAM-1 , IL-6 , IL-a and TNF-a
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1997-01-01
2021-05-13
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References

  1. Arai K., Lee F., Miyajima A., Shoichiro M., Arai N., Yokota T. 1990; Cytokines: coordinators of immune and inflammatory responses.. Annu Rev Biocbem 59:783–793
    [Google Scholar]
  2. Buchmeier N.A., Heffron F. 1990; Induction of Salmonella stress proteins upon infection of macrophages.. Science 248:730–732
    [Google Scholar]
  3. Cerami A., Beutler B. 1988; The role of cachectin/TNF in endotoxic shock and cachexia.. Immunol Today 9:28–31
    [Google Scholar]
  4. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.. Anal Biochem 162:156–159
    [Google Scholar]
  5. Clerget M., Polla B.S. 1990; Erythrophagocytosis induces heat shock protein synthesis by human monocytes-macrophages.. Proc Natl Acad Sci USA 871081–1085
    [Google Scholar]
  6. Damle N.K., Aruffo A. 1991; Vascular cell adhesion molecule 1 induces T-cell antigen receptor-dependent activation of CD4+ T lymphocytes.. Proc Natl Acad Sci USA 886403–6407
    [Google Scholar]
  7. Dinarello C.A., Cannon J.C., Wolff S.M., Bernheim H.A., Beutler B., Cerami A., Figari I.S., Palladino M.A. Jr O’Connor J.V. 1986; Tumor necrosis factor (cachetin) is an endogenous pyrogen and induces production of Interleukin-1.. J Exp Med 163:1433–1450
    [Google Scholar]
  8. Dunlop L.C., Skinner M.P., Bendall L.J., Favaloro J., Castaldi P.A., Gormann J.J., Gamble J.R., Vadas M.A., Berndt M.C. 1992; Characterization of GMP-140 (P-selectin) as a circulating plasma protein.. J Exp Med 175:1147–1150
    [Google Scholar]
  9. Dustin M.L., Rothlein R., Bhan A.K., Dinarello C.A., Springer T.A. 1986; Induction by IL-1 and interferon gamma, tissue distribution, biochemistry and function of a natural adherence molecule (ICAM-1).. J Immunol 137:245–254
    [Google Scholar]
  10. Ensgraber M., Loss M. 1992; A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus.. Infect Immun 60:3072–3078
    [Google Scholar]
  11. Fayet O., Ziegelhoffer T., Georgopoulos C. 1989; The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures.. J Bacteriol l71:1379–1385
    [Google Scholar]
  12. Gamble J.R., Skinner M.P., Berndt M.C., Vadas M.A. 1990; Prevention of activated neutrophil adhesion to endothelium by soluble adhesion protein GMP 140.. Science 249:414–417
    [Google Scholar]
  13. Georgopoulos C., Welch W.J. 1993; Role of the major heat shock proteins as molecular chaperones.. Annu Rev Cell Biol 9:601–634
    [Google Scholar]
  14. Gromkowski S.H., Yagi J., Janeway C.A. Jr 1989; Elevated temperature regulates tumor necrosis factor-mediated immune killing.. Eur J Immunol 19:1709–1714
    [Google Scholar]
  15. Groves R.W., Ross E., Barker J.N.W.N., Ross J.S., Camp R.D.R., MacDonald D.M. 1992; Effect of in vivo interleukin-1 on adhesion molecule expression in normal human skin.. J Invest Dermatol 98:384–387
    [Google Scholar]
  16. Hemmingsen S.M., Woolford C., VanderVies S.M., Tilly K., Dennis D.T., Georgopoulos C.P., Hendrix R.W., Ellis R.J. 1988; Homologous plant and bacterial proteins chaperone oligomeric protein assembly.. Nature 333:330–334
    [Google Scholar]
  17. Karlsson-Parra A., Söderström K., Ferm M., Ivanyi J., Kiessling R., Klareskog L. 1990; Presence of human 65 kD heat shock protein (hsp) in inflamed joints and subcutaneous nodules of RA patients.. Scand J Immunol 31:283–288
    [Google Scholar]
  18. Kaufmann S.H.E. 1990; Heat shock proteins and the immune response.. Immunol Today 11:129–136
    [Google Scholar]
  19. Kaufmann S.H.E., Schoel B., Van Embden J.D.A., Koga T., Wand-Württenberger A., Munk M.E., Steinhoff U. 1991; Heat shock protein 60: implications for pathogenesis of and protection against bacterial infections.. Immunol Rev 121:67–90
    [Google Scholar]
  20. Köch A., Schwarz T., Kirnbauer R., Urbanski A., Perry P., Ansel J.C., Luger T.A. 1990; Human keratinocytes are a source for tumor necrosis factor α: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light.. J Exp Med 172:1609–1614
    [Google Scholar]
  21. Koga T., Wand-Württenberger A., DeBruyn J., Munk M.E., Schoel B., Kaufmann S.H.E. 1989; T cells against a bacterial heat shock protein recognize stressed macrophages.. Science 245:1112–1115
    [Google Scholar]
  22. Kohno T., Brewer M.T., Baker S.L., Schwartz P.E., King P.E., Hale K.K., Squires C.H., Thompson C.H., Vannice J.N. 1990; A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor.. Proc Natl Acad Sci USA 878331–8335
    [Google Scholar]
  23. Kupper T.S. 1990; The role of epidermal cytokines.. In The Immunophysiology of Cells and Cytokines pp. 285–306 Oppenheim J. J., Shevach E. Edited by New York:: Oxford University Press.;
    [Google Scholar]
  24. Lindquist S., Craig E.A. 1989; The heat-shock proteins.. Annu Rev Genet 22:631–637
    [Google Scholar]
  25. Lo S.K., Lee S., Ramos R.A., Lobb R., Rosa M., Chi-Rosso G., Wright S.D. 1991; Endothelial-leukocyte adhesion molecule 1stimulates the adhesive activity of leukocyte integrin CR3 (Cdllb/CD18, Mac-1, α m β 2) on human neutrophils.. J Exp Med 173:1493–1500
    [Google Scholar]
  26. Luger T.A., Schwarz T. 1990; Evidence for an epidermal cytokine network.. J Invest Dermatol 95:1005–1011
    [Google Scholar]
  27. Mannel D.N., Moore R.N., Mergenhagen S.E. 1990; Macrophages as a source of tumoricidal activity (tumor-necrotizing factor).. Infect Immun 30:523–530
    [Google Scholar]
  28. Marlin S.D., Springer T.A. 1987; Purified intercellular adhesion molecule 1 (ICAM-1) is a ligand for lymphocyte function associated antigen 1 (LFA-1).. Cell 51:813–819
    [Google Scholar]
  29. Nickoloff B.J., Karabin G.D., Barker J.N.W.N., Griffiths C.E.M., Sarrua V., Mitra R.S., Elder J.T., Kruker S.L., Dikit V.M. 1991; Cellular localization of interleukin-8 and its inducer, tumor necrosis factor-alpha in psoriasis.. Am J Pathol 138:129–134
    [Google Scholar]
  30. Partridge M., Chantry D., Turner M., Feldmann M. 1991; Production of interleukin-1 and interleukin-6 by human keratinocytes and squamous cell carcinoma cell lines.. J Invest Dermatol 96:771–776
    [Google Scholar]
  31. Peetermans W. E., Raats C. J. I., van Furth R., Langermans J. A. M. 1995a; Mycobacterial 65-kilodalton heat shock protein induces tumor necrosis alpha and interleukin 6, reactive nitrogen intermediates, and toxoplasmatic activity in murine peritoneal macrophages.. Infect Immun 63:3454–3458
    [Google Scholar]
  32. Peetermans W. E., D’Haens G. R., Ceuppens J. L., Rutgeerts P., Geboes K. 1995b; Mucosal expression by B7-positive cells of the 60-kilodalton heat-shock protein in inflammatory bowel disease.. Gastroenterology 108:75–82
    [Google Scholar]
  33. Pelham H. 1988; Heat-shock proteins. Coming in from the cold.. Nature 332:776–777
    [Google Scholar]
  34. Polla B.S. 1988; A role for heat shock proteins in inflammation ?. Immunol Today 5:134–137
    [Google Scholar]
  35. Retzlaff C., Yamamoto Y., Hoffman P.S., Friedman H., Klein T.W. 1994; Bacterial heat shock proteins directely induce cytokine mRNA and interleukin-1 secretion in macrophage cultures.. Infect Immun 62:5689–5693
    [Google Scholar]
  36. Sauder D.N., Stanulis-Praeger B.M., Gilchrest B.A. 1988; Autocrine growth stimulation of human keratinocytes by epidermal cell-derived thymocyte-activating factor: implications for skin aging.. Arch Dermatol Res 280:71–76
    [Google Scholar]
  37. Seth R., Raymond F.D., Makgoba M.W. 1991; Circulating ICAM-1 isoforms: diagnostic prospects for inflammatory and immune disorders.. Lancet 338:83–84
    [Google Scholar]
  38. Shalaby M.R., Aggarwall B.B., Rinderknecht E., Svedersky L.P., Finkle B.S., Palladino M.A. Jr 1984; Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factor.. J Immunol 135:2069–2073
    [Google Scholar]
  39. Singer K.H., Tuck D.T., Sampson H.A., Hall R.P. 1989; Epidermal keratinocytes express the adhesion molecule intercellular adhesion molecule-1 in inflammatory dermatoses.. J Invest Dermatol 93:746–750
    [Google Scholar]
  40. Smith C.W., Kishimoto T.K., Abbass O., Hughes B., Rothlein R., Mclntire L.V., Butcher E., Anderson D.C. 1991; Chemo- tactic factors regulate lectin adhesion molecule 1 (LECAM-1)- dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro. . J Clin Invest 87:609–618
    [Google Scholar]
  41. Trefzer U., Brockhaus M., Loetscher H., Parlow F., Kapp A., Schöpf E., Krutmann J. 1991; 55-kd tumor necrosis factor receptor is expressed by human keratinocytes and plays a pivotal role in regulation of human keratinocyte ICAM-1 expression.. J Invest Dermatol 97:911–916
    [Google Scholar]
  42. Walev I., Martin E., Jonas D., Mohamadzadeh M., Muller-Klieser W., Kunz L., Bhakdi S. 1993; Staphylococcal alpha- toxin kills human keratinocytes by permeabilizing the plasma membrane for monovalent ions.. Infect Immun 61:4972–4979
    [Google Scholar]
  43. Zhang Y., Doerfler M., Lee T.C., Guillemin B., Rom W. 1993; Mechanisms of stimulation of interleukin-1β and tumor necrosis factorα by Mycobacterium tuberculosis components.. J Clin Invest 91:2076–2083
    [Google Scholar]
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