1887

Abstract

A 44 kDa protein is a dominant component of periplasmic extracts of . Peptide sequence generated from a cyanogen-bromide-cleaved fragment of this protein indicated sequence homology with elongation factor-Tu (EF-Tu). Polyclonal antiserum was made against the 44 kDa protein purified from periplasm extracts of . The preabsorbed antiserum was immunoblotted against whole-cell lysates on two-dimensional gels. A 44 kDa protein and a smaller 37 kDa protein were recognized by this antiserum. A λ phage DNA library was screened and a clone expressing a 44 kDa protein was identified. The DNA insert in this clone contained several genes homologous to genes contained in the operon of One ORF product with a calculated molecular mass of 43 kDa was highly homologous to the EF-TuA of A synthetic peptide antiserum specific for a portion of the C terminus of EF-Tu confirmed that the 37 kDa protein in whole-cell lysates of was a processed form of EF-Tu. Deletion of the gene homologue in was attempted but was unsuccessful.

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1996-09-01
2021-05-14
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