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Volume 141, Issue 1

An analysis of the instability kinetics of plasmid pHSG415 during continuous culture of Free

Abstract

SUMMARY

The effect of dilution rate on the instability kinetics of RV308(pHSG415) during glucose-limited continuous culture is examined. Two nonlinear models are fitted to the data, both of which characterize the plasmid-host system in terms of the rate parameters (for the plasmid segregation rate) and (for the specific growth rate difference between plasmid-free and plasmid-bearing single cells). In the first model, both and have constant values with respect to time. In the second, either or is represented as a time-dependent function. Although both models fit the data equally well, it is demonstrated that the constant rate parameter model gives results which appear to be misleading. A comparison is also made among some of the many plasmid instability models (both mass-balance and segregated) which have appeared in the literature. It is found that all of these give identical trajectories and differ only in the definitions of the rate parameters used.

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Keyword(s): mathematical model and plasmid instability
© Society for General Microbiology 1995
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1995-01-01
2023-04-02
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An analysis of the instability kinetics of plasmid pHSG415 during continuous culture of Escherichia coli
Microbiology 141, 63 (1995); https://doi.org/10.1099/00221287-141-1-63
/content/journal/micro/10.1099/00221287-141-1-63
/content/journal/micro/10.1099/00221287-141-1-63
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