1887

Abstract

SUMMARY: An efficient electrotransformation system for Streptococcus type 2 is described. It is demonstrated that vectors based on the broad-host-range plasmid pWVO1 replicate in type 2. Transformation efficiencies of about 10 transformants per μg of plasmid DNA could be obtained. Derivatives of plasmid pBR322 containing chromosomal DNA did not replicate but integrated into the chromosome. Southern hybridization analysis revealed that double as well as single cross-over integration events had occurred. Double cross-over events occurred at a frequency of about 15%. With these transformation and integration systems, recombinant DNA technology can now be applied to this important pathogenic species.

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/content/journal/micro/10.1099/00221287-141-1-181
1995-01-01
2021-05-13
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