1887

Abstract

The response of derepressed cells of to the addition of glucose included a marked and reversible activation of neutral trehalase that was not produced in repressed cells. The protein synthesis inhibitor cycloheximide, the protonophore 2,4-dinitrophenol or the uncoupler sodium azide also enhanced trehalase activity in derepressed cells provided glucose was present in the incubation assays. However, only 2,4-dinitrophenol or cycloheximide was able to induce trehalase activation in repressed cells. Stimulation of trehalase by these compounds was preceded in all cases by a rapid increase in adenosine 3′-5′-cyclic monophosphate (cAMP) content. Since exogenous cAMP can activate trehalase both in repressed and derepressed growing cells, the results provide evidence for the existence of an induced cAMP signalling pathway in the fission yeast with several entries for trehalase activation. The correlation between cAMP increase and trehalase activation was not maintained when the enzyme was heat-shock-activated, supporting the concept that trehalase activity can be also enhanced in cells by another mechanism in which cAMP does not act as second messenger.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-6-1467
1994-06-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/6/mic-140-6-1467.html?itemId=/content/journal/micro/10.1099/00221287-140-6-1467&mimeType=html&fmt=ahah

References

  1. Arguelles J.C., Gacto M. 1985; Evidence for regulatory trehalase activity in Candida utilis. Can J Microbiol 31:529–537
    [Google Scholar]
  2. Arkinstall S. J., Papasavvas S. G., Payton M. A. 1991; Yeast a-mating factor receptor-linked G-protein signal transduction suppresses Raj-dependent activity. FEBS Lett 284:123–128
    [Google Scholar]
  3. Byrne S.M., Hoffman C. S. 1993; Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomjces pombe. J Cell Sci 105:1095–1100
    [Google Scholar]
  4. Carrillo D., Vicente-Soler J., Gacto M. 1992; Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccbaromyces pombe. FEMS Microbiol Lett 98:61–66
    [Google Scholar]
  5. De Virgilio C., Simmen U., Hottiger T., Boiler T., Wiemken A. 1990; Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schispsaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett 273:107–110
    [Google Scholar]
  6. De Virgilio C., Muller J., Boiler T., Wiemken A. 1991; A constitutive, heat-shock activated neutral trehalase occurs in Schipsaccharomycespombe in addition to the sporulation-specific acid trehalase. FEMS Microbiol Lett 84:85–90
    [Google Scholar]
  7. Dewerchin M.A., Van Laere A. J. 1984; Trehalase activity and cyclic AMP content during early development of Mucor rouxii spores. J Bacteriol 158:575–579
    [Google Scholar]
  8. Engelberg D., Poradosu E., Simchem G., Levitzki A. 1990; Adenylyl cyclase activity of the fission yeast Schipsaccharomyces pombe is not regulated by guanyl nucleotides. FEBS Lett 261:413–418
    [Google Scholar]
  9. Fukui Y., Kozasa T., Kajiro Y., Takeda T., Yamamoto M. 1986; Role of a ras homologue in the life cycle of Schip- saccharomyces pombe. Cell 44:329–336
    [Google Scholar]
  10. Gibbs J.B., Marshall M.S. 1989; The ras oncogene - an important regulatory element in lower eucaryotic organisms. Microbiol Rev 53:171–185
    [Google Scholar]
  11. Hoffman C.S., Winston F. 1991; Glucose repression of transcription of the Schipsaccharomyces pombe fbp 1 gene occurs by a cAMP signalling pathway. Genes and Dev 5:561–571
    [Google Scholar]
  12. Hirimburegama K., Durnez P., Keleman J., Oris E., Vergauwen R., Mergelsberg H., Thevelein J. M. 1992; Nutrient-induced activation of trehalase in nutrient-starved cells of the yeast Saccbaromyces cerevisiae: cAMP is not involved as second messenger. J Gen Microbiol 138:2035–2043
    [Google Scholar]
  13. McDonald I. J., Tsai C. S. 1989; Continuous culture and intermediary carbon metabolism. In Molecular Biology of the Fission Yeast pp. 367–396 Edited by Nasim A., Young P., Johnson B. F. . New York: Academic Press;
    [Google Scholar]
  14. Ortiz C. H., Maia J. C. C., Tenan M. R., Braz-Padrao G. R., Mattoon G. R., Panek A. D. 1983; Regulation of yeast trehalase by a monocyclic, cyclic AMP-dependent phosphorylation- dephosphorylation cascade system. J Bacteriol 153:644–651
    [Google Scholar]
  15. Russell P., Nurse P. 1986; Schipsaccharomyces pombe and Saccbaromyces cerevisiae: a look at yeast divided. Cell 45:781–782
    [Google Scholar]
  16. Schlanderer G., Dellweg H. 1974; Cyclic AMP and catabolite repression in yeasts. Eur J Biochem 49:305–316
    [Google Scholar]
  17. Thevelein J.M. 1991; Fermentable sugars and intracellular acidification as specific activators of the RAS-adenylate cyclase signalling pathway in yeast: the relationship to nutrient-induced cell cycle control. Mol Microbiol 5:1301–1307
    [Google Scholar]
  18. Thevelein J.M. 1992; The RAS-adenylate cyclase pathway and cell cycle life control in Saccbaromyces cerevisiae. Antonie Leeuwenhoek. J Microbiol special issue Molecular Biology of Yeasts. Edited by Grivell L. 62:109–130
    [Google Scholar]
  19. Thevelein J.M., Beullens M. 1985; Cyclic AMP and the stimulation of trehalase activity in the yeast Saccbaromyces cerevisiae by carbon sources, nitrogen sources and inhibitors of protein synthesis. J Gen Microbiol 131:3199–3209
    [Google Scholar]
  20. Uno I., Matsumoto K., Adachi K., Ishikawa T. 1983; Genetic and biochemical evidence that trehalase is a substrate of cAMP- dependent protein kinase in yeast. J Biol Chem 258:10867–10872
    [Google Scholar]
  21. Van Mulders R. M., Van Laere A. J. 1984; Cyclic AMP, trehalase and germination of P by corny ces blakesleeanus spores. J Gen Microbiol 130:541–547
    [Google Scholar]
  22. Wills C. 1990; Regulation of sugar and ethanol metabolism in Saccbaromyces cerevisiae. Crit Rev Biochem Mol Biol 25:245–280
    [Google Scholar]
  23. Yamakawi-Kataoka Y, Tamaoki T., Choe H. R., Tanaka H., Kataoka T. 1989; Adenylate cyclases in yeast: a comparison of the genes from Schipsaccharomyces pombe and Saccbaromyces cerevisiae. Proc Natl Acad Sci USA 86:5693–5697
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-6-1467
Loading
/content/journal/micro/10.1099/00221287-140-6-1467
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error