1887

Abstract

A DNA segment cloned from ATCC 25489 close to a region that hybridized to a probe containing part of the actinorhodin polyketide synthase caused ATCC 31615 to produce new anthracyclines. When transformed with certain sub-clones of this segment, the host produced glycosides of β-rhodomycinone,β-rhodomycinone, 10-demethoxycarbonylaklavinone and 11-deoxy-β-rhodomycinone in addition to those of aklavinone, the natural anthracyclines of . The first two compounds are products and the other two are novel compounds that conceptually are structural hybrids between and products. Three glycosides of one of the novel aglycones, 11-deoxy-β-rhodomycinone, were purified and found to possess cytotoxic activity against L1210 mouse leukaemia cells. Separate regions of the cloned DNA are responsible for modification of the host product at the 10- and 11-positions.

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1994-06-01
2021-10-17
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