1887

Abstract

The conversion of pyruvate to lactate is a key feature of lactococcal strains. The enzyme which facilitates this conversion, -lactate dehydrogenase (LDH), and the gene which encodes it (), are therefore of great significance. This paper presents the cloning and DNA sequence analysis of three further lactococcal genes which are of key importance in the genetic manipulation of commercial starter strains. The gene from subsp. biovar BU2-60 has been isolated from a lambda library and sequenced. The gene from subsp. NCDO 762 and that from subsp. IL1403 have been amplified by the polymerase chain reaction (PCR) and sequenced. These DNA sequences and deduced amino acid sequences have been compared with those from subsp. MG1363. The LDHs from subsp. MG1363 and subsp. NCDO 762 are 99·4% homologous. The LDHs from subsp. MG1363 and subsp. IL1403 are 96·4% homologous. The LDHs from subsp. IL1403 and subsp. biovar BU2-60 are 99,9% homologous. Our results provide further evidence that subsp. MG1363 and other subsp. NCDO 712 derived strains should be reclassified as subsp.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-6-1301
1994-06-01
2021-05-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/6/mic-140-6-1301.html?itemId=/content/journal/micro/10.1099/00221287-140-6-1301&mimeType=html&fmt=ahah

References

  1. Barstow D. A., Clarke A. R., Chia W. N., Wigley D., Sharman A. F., Holbrook J. J., Atkinson T., Minton N. P. 1986; Cloning, expressioncomplete nucleotide sequence of the Bacillus stearothermophilus L-lactate dehydrogenase gene. Gene 46:47–55
    [Google Scholar]
  2. Barstow D. A., Murphy J. P., Sharman A. F., Clarke A. R., Holbrook J. J., Atkinson T. 1987; Amino acid sequence of the L-lactate dehydrogenase of Bacillus caldotenax deduced from the nucleotide sequence of the cloned gene. Eur J Biochem 165:581–586
    [Google Scholar]
  3. Casadaban M.J., Cohen S. N. 1980; Analysis of gene control signals by DNA fusioncloning in E. coli. J Mol Biol 138:179–207
    [Google Scholar]
  4. Chopin A., Chopin M.-C., Moillo-Batt A., Langella P. 1984; Two plasmid determined restrictionmodification systems in Streptococcus lactis. Plasmid 11:260–263
    [Google Scholar]
  5. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Non-chromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R-factor DNA. Proc Natl Acad Sci ESA 69:2110–2114
    [Google Scholar]
  6. Duwat P., Ehrlich S. D., Gruss A. 1992; Use of degenerate primers for polymerase chain-reaction cloningsequencing of the Lactococcus lactis subsp. lactis rec A gene. Appl Environ Microbiol 58:2674–2678
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies; an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  8. Feng D.F., Doolittle R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. Mol Evol 25:351–360
    [Google Scholar]
  9. Gasson M.J. 1983; Plasmid complements of Streptococcus lactis NCDO 712other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1–9
    [Google Scholar]
  10. Godon J. J., Delorme C., Ehrlich S. D., Renault P. 1992; Divergence of genomic sequences between Lactococcus lactis subsp. lactisLactococcus lactis subsp. cremoris. Appl Environ Microbiol 58:4045–4047
    [Google Scholar]
  11. Griffin H. G., Swindell S. R., Gasson M. J. 1992; Cloningsequence analysis of the gene encoding L-lactate dehydrogenase from Lactococcus lactis: evolutionary relationships between 21 different LDH enzymes. Gene 122:193–197
    [Google Scholar]
  12. Griffin H. G., I'anson K. J., Gasson M. J. 1993; Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening. FEMS Microbiol Lett 112:49–53
    [Google Scholar]
  13. Haldimann A., Nicolet J., Frey J. 1993; DNA sequence determinationbiochemical analysis of the immunogenic protein P36, the lactate dehydrogenase (LDH) of Mycoplasma hyopneumoniae. J Gen Microbiol 139:317–323
    [Google Scholar]
  14. Hanahan D. 1985; Techniques for transformation of E. coli. In DNA Cloning, pp. 1:109–135 Edited by Glover D. M. Washington Dc: IRL Press;
    [Google Scholar]
  15. Hillman J. D., Duncan M. J., Stashenko K. P. 1990; Cloningexpression of the gene encoding fructose-1,6-diphosphate- dependent l-( + )-lactate dehydrogenase of Streptococcus mutans. Infect Immun 58:1290–1295
    [Google Scholar]
  16. Jarvis A.W., Jarvis B. D. W. 1981; Deoxyribonucleic-acid homology among lactic streptococci. Appl Environ Microbiol 41:77–83
    [Google Scholar]
  17. Kim S. F., Baek S. J., Pack M. Y. 1991; Cloningnucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene. Appl Environ Microbiol 57:2416–2417
    [Google Scholar]
  18. Mayo B., Kok J., Venema K., Bockelmann W., Teuber M., Reinke H., Venema G. 1991; Molecular-cloningsequence- analysis of the X-prolyl dipeptidyl aminopeptidase gene from Lactococcus lactis subsp cremoris. Appl Environ Microbiol 57:38–44
    [Google Scholar]
  19. Minowa T., Iwata S., Sakai H., Masaki H., Ohta T. 1989; Sequencecharacteristics of the Bifidobacterium longum gene encoding L-lactate dehydrogenasethe primary structure of the enzyme: a new feature of the allosteric site. Gene 85:161–168
    [Google Scholar]
  20. Nardi M., Chopin M. C., Chopin A., Cals M. M., Gripon J. C. 1991; CloningDNA-sequence analysis of an X-prolyl dipep-tidyl aminopeptidase gene from Lactococcus lactis subsp. lactis NCDO 763. Appl Environ Microbiol 57:45–50
    [Google Scholar]
  21. Neve H., Geis A., Teuber M. 1984; Conjugal transfercharacterisation of bacteriocin plasmids in group N (lactic acid) streptococci. J Bacteriol 157:833–838
    [Google Scholar]
  22. Salama M., Sandine W., Giovannoni S. 1991; Developmentapplication of oligonucleotide probes for identification of Lactococcus lactis subsp. cremoris. Appl Environ Microbiol 57:1313–1318
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2 nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Taguchi H., Ohta T. 1991; D-Lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencingexpression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum. J. Biol Chem 266:12588–12594
    [Google Scholar]
  25. Terzaghi J., Sandine W. E. 1975; Improved media for lactic streptococcitheir bacteriophages. Appl Microbiol 29:807–813
    [Google Scholar]
  26. Vckovski V., Schlatter D., Zuber H. 1990; Structurefunction of L-lactate dehydrogenases from thermophilicmesophilic bacteria. IX. Identification, isolationnucleotide sequence of two L-lactate dehydrogenase genes of the psychrophilic bacterium Bacillus psychrosaccharolyticus. Biol Chem Hoppe-Seyler 371:103–110
    [Google Scholar]
  27. Waldvogel S., Weber H., Zuber H. 1987; Structurefunction of L-lactate dehydrogenases from thermophilicmesophilic bacteria. VII. Nucleotide sequence of the lactate dehydrogenase gene from the mesophilic bacterium Bacillus megaterium. Preparationproperties of a hybrid lactate dehydrogenase comprising moieties of the B. megateriumB. stearothermophilus enzymes. Biol Chem Hoppe-Seyler 368:1391–1399
    [Google Scholar]
  28. Zulli F., Weber H., Zuber H. 1987; Structurefunction of l- lactate dehydrogenases from thermophilicmesophilic bacteria, VI. Nucleotide sequences of lactate dehydrogenase genes from the thermophilic bacteria Bacillus stearothermophilusB. caldolyticusB. caldotenax. Biol Chem Hoppe-Seyler 368:1167–1177
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-6-1301
Loading
/content/journal/micro/10.1099/00221287-140-6-1301
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error