1887

Abstract

The region of biovar had originally been recognized on the basis of its ability to repress the transcription of the psi genes, one of which, , inhibits exopolysaccharide synthesis when cloned in multi-copy plasmids. Both psr and psi are located on the symbiotic plasmid pRP2JI. The gene was localized and sequenced. The deduced amino acid sequence of PsrA was shown to have similarity to the DNA-binding region of a family of other transcriptional regulators, consistent with its known effects on the expression of psi. The transcription of itself appears to be constitutive in free-living , but is regulated by another gene on the Sym plasmid pRP2JI.

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1994-03-01
2024-11-05
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References

  1. Bagdasarian M., Lurz R., Ruckert B., Franklin F.C.H., Bagda-Sarian M.M., Frey J., Timmis K.T. Specific-purpose plasmid cloning vectors II Broad host range, high copy RSF1010- derived vectors and a host-vector system for gene cloning in Pseudomonas. Gene 1981; 16:237–247
    [Google Scholar]
  2. Beringer J.E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 1974; 83:188–198
    [Google Scholar]
  3. Borthakur D., Johnston A.W.B. Sequence ofpsi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid. Mol & Gen Genet 1987; 207:149–154
    [Google Scholar]
  4. Borthakur D., Barber C.E., Lamb J.W., Daniels M.J., Downie J.A., Johnston A.W.B. A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas. Mol & Gen Genet 1986; 203:320–323
    [Google Scholar]
  5. Borthakur D., Barker R.F., Latchford J.W., Rossen L., Johnston A.W.B. Analysis of pss genes of Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: their primary structure and their interaction with psi and other nodulation genes. Mol & Gen Genet 1988; 213:155–162
    [Google Scholar]
  6. Borthakur D., Downie J.A., Johnston A.W.B., Lamb J.W. psi, a plasmid-linked Rhipobium phaseoli gene that inhibits exopolysaccharide production and which is required for symbiotic nitrogen fixation. Mol & Gen Genet 1985; 200:278–282
    [Google Scholar]
  7. Buchanan-Wollaston A.V., Beringer J.E., Brewin N.J., Hirsch P.R., Johnston A.W.B. Isolation of symbiotically defective mutants in Rhizobium leguminosarum by insertion of the transposon Tn5 into a transmissible plasmid. Mol & Gen Genet 1980; 178:185–190
    [Google Scholar]
  8. Davis E.O., Johnston A.W.B. Analysis of three nodD genes in Rhizobium leguminosarum biovar phaseoli; nodD 1 is preceded by nolE, a gene whose product is secreted from the cytoplasm. Mol Microbiol 1990a; 4:921–932
    [Google Scholar]
  9. Davis E.O., Johnston A.W.B. Regulatory functions of the three nodD genes of Rhispbium leguminosarum biovar phaseoli. Mol Microbiol 1990b; 4:933–941
    [Google Scholar]
  10. Doherty D., Leigh J.A., Glazebrook J., Walker G.C. Rhiqpbium meliloti mutants that over produce the R meliloti acidic calcofluor-binding exopolysaccharide. J Bacteriol 1988; 170:4249–56
    [Google Scholar]
  11. Finan T.M., Kunkel B., De Vos G.F., Signer E.R. Second symbiotic megaplasmid in Rhiipbium meliloti carrying exopolysaccharide and thiamine synthesis genes. J Bacteriol 1986; 167:66–72
    [Google Scholar]
  12. Friedrich M.J., Kadner R.J. Nucleotide sequence of the uhp region of Escherichia coli. J Bacteriol 1987; 169:3556–3563
    [Google Scholar]
  13. Gicquel-Sanzey B., Crossart P. Homologies between different prokaryotic DNA-binding regulatory proteins and between their sites of action. EMBO J 1982; 1:591–595
    [Google Scholar]
  14. Gray J.X., Rolfe B.G. Exopolysaccharide production in Rhipbium and its role in invasion. Mol Microbiol 1990; 4:1425–31
    [Google Scholar]
  15. Gray J.X., Djordjevic M.A., Rolfe B.G. Two genes that regulate exopolysaccharide production in Rhirpbium sp strain NGR234: DNA sequences and resultant phenotypes. J Bacteriol 1990; 172:193–203
    [Google Scholar]
  16. Hawkins F.K.L., Johnston A.W.B. Transcription of a Rhispbium leguminosarum biovar phaseoli gene needed for melanin synthesis is activated by nip A of Rhispbium and Klebsiella pneumoniae. Mol Microbiol 1988; 2:331–337
    [Google Scholar]
  17. Hotter G.S., Scott D.B. Exopolysaccharide mutants of Rhispbium loti are fully effective on a determinate nodulating host but are ineffective on an indeterminate nodulating host. J Bacteriol 1991; 173:851–59
    [Google Scholar]
  18. Hynes M.F., Simon R., Muller P., Niehaus K., Labes M., Puhler A. The two megaplasmids of Rhipbium meliloti are involved in the effective nodulation of alfalfa. Mol & Gen Genet 1986; 202:356–62
    [Google Scholar]
  19. Kolling R., Lother H. AsnC: an autogenously regulated activator of asparaginase synthetase A transcription in Escherichia coli. J Bacteriol 1985; 164:310–315
    [Google Scholar]
  20. Labes M., Puhler A., Simon R. A new family of RSFlOlO-derived expression and lac fusion broad host-range vectors for Gram negative bacteria. Gene 1990; 89:37–46
    [Google Scholar]
  21. Lamb J.W., Hombrecher G., Johnston A.W.B. Plasmid- determined nodulation and nitrogen-fixation abilities in Rhispbium phaseoli. Mol & Gen Genet 1982; 186:449–452
    [Google Scholar]
  22. Lamb J.W., Downie J.A., Johnston A.W.B. Cloning of the nodulation (nod) genes of Rhipbium phaseoli and their homology to R. leguminosarum nod DNA. Gene 1985; 34:235–241
    [Google Scholar]
  23. Latchford J.W., Borthakur D., Johnston A.W.B. The products of Rhitpbium genes, psi and pss, which affect exopolysaccharide production, are associated with the bacterial cell surface. Mol Microbiol 1991; 5:2107–2114
    [Google Scholar]
  24. Leigh J.A., Coplin D.L. Exopolysaccharides in plant- bacterial interactions. Annu Rev Microbiol 1992; 46:307–46
    [Google Scholar]
  25. Leigh J.A., Reed J.W., Hanks J.F., Hirsch A.M., Walker G.C. Rhispbium meliloti mutants that fail to succinylate their calcofluor-binding exopolysaccharide are defective in nodule invasion. Cell 1987; 51:579–87
    [Google Scholar]
  26. Leigh J.A., Signer E.R., Walker G.C. Exopolysaccharide-deficient mutants of Rhiipbium meliloti that form ineffective nodules. Proc Natl Acad Sci USA 1985; 82:6231–35
    [Google Scholar]
  27. Ljungquist E., Kockum K., Bertani E.L. DNA sequences of the repressor gene and operator region of bacteriophage P2. Proc Natl Acad Sci USA 1984; 81:3988–3992
    [Google Scholar]
  28. Long S., Reed J.W., Himawan J., Walker G.C. Genetic analysis of a cluster of genes required for synthesis of the calcofluor- binding exopolysaccharide of Rhizpbium meliloti. J Bacteriol 1988; 170:4239–48
    [Google Scholar]
  29. Makino K., Shinagawa H., Amemura M., Nakata A. Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12. J Mol Biol 1986; 190:37–44
    [Google Scholar]
  30. Maniatis T., Fritsch E.F., Sambrook J. Molecular Cloning: A Laboratory Manual 1982 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Pabo C.O., Sauer R.T. Protein-DNA recognition. Annu Rev Biochem 1984; 53:293–321
    [Google Scholar]
  32. Reed J.W., Capage M., Walker G.C. Rhizobium meliloti exoG and exoj mutations affect the ExoX-ExoY system for modulation of exopolysaccharide production. J Bacteriol 1991a; 173:3776–88
    [Google Scholar]
  33. Reed J.W., Glazebrook J., Walker G.C. The exoR gene of Rhispbium meliloti affects RNA levels of other exo genes but lacks homology to known transcriptional regulators. J Bacteriol 1991b; 173:3789–94
    [Google Scholar]
  34. Reuber T.L., Long S., Walker G.C. Regulation of Rhitpbium meliloti exo genes in free-living cells and in planta examined by using TnphoA fusions. J Bacteriol 1991a; 173:426–34
    [Google Scholar]
  35. Reuber T.L., Reed J., Glazebrook J., Glucksman M.A., Ahmann D., Waller G.C. Rhitpbium meliloti exopolysaccharides: genetic analyses and symbiotic importance. Biochem Soc Trans 1991b; 19:636–41
    [Google Scholar]
  36. Rossen L., Shearman C.A., Johnston A.W.B., Downie J.A. The nodD gene of Rhispbium leguminosarum is autoregulatory and in the presence of plant exudate induces the nodABC genes. EMBO J 1985; 4:3369–3373
    [Google Scholar]
  37. Shaw D.J., Rice D.W., Guest J.R. Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli. J Bacteriol 1983; 166:241–247
    [Google Scholar]
  38. Spaink H.P., Okker J.H., Wijffelman C.A., Pees E., Lugtenberg B.J.J. Promoters in the nodulation region of the Rhirpbium leguminosarum Sym plasmid pRLl JI. Plant Mol Biol 1987; 9:27–39
    [Google Scholar]
  39. Tulley R.E., Terry M.E. Decreased exopolysaccharide synthesis by anaerobic and symbiotic cells of Bradyrhigobium japonicum. Plant Physiol 1985; 79:445–450
    [Google Scholar]
  40. Valentin-Hansen P., Hojrup P., Short S. The primary structure of the DeoR repressor from Escherichia coli K-12. Nucleic Acids Res 1985; 13:5926–5936
    [Google Scholar]
  41. Wood W.B. Host specificity of DNA produced by E. coli bacterial mutations affecting the restriction and modification of DNA. J Mol Biol 1966; 16:118–133
    [Google Scholar]
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