Lipopolysaccharides (LPS) from serogroup 1, serogroup 1 and were subjected to chemical analysis. All three LPS contained D-mannose, D-glucose, D-glucosamine, L--D--heptose, 2-keto-3-deoxyoctonic acid and glycerol. In addition the LPS of was characterized by L-quinovose (tentatively identified) and L-fucosamine, LPS by D-quinovosamine, D-galactosamine and D-galacturonic acid, and LPS by D-quinovosamine. Phosphorylated sugars were detected in all three LPS. The backbone sugar of the lipid A part was in each case 2,3-diamino-2,3-dideoxy-D-glucose substituted with a complex pattern of fatty acid, including 20-22 different amide-linked (non-branched and methyl-branched) 3-hydroxy fatty acids of chain-length ranging from 12 to 23 carbon atoms. The fatty acid patterns included also ester-linked nonhydroxylated entities and the uncommon 27-oxo-octacosanoic acid and 29-oxotriacontanoic acid. The LPS of and also contained heptacosane-1,27-dioic and nonacosane-1,29-dioic acid, and their 2-hydroxy analogues were characteristic of LPS. SDS-PAGE patterns of the three LPS were distinctly different. Both and produced smooth-form LPS with characteristic ladder patterns, whereas LPS were of more rough-type character.


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