1887

Abstract

SUMMARY: The three different pore-forming RTX-toxins of are reviewed, and new and uniform designations for these toxins and their genes are proposed. The designation ApxI (for RTX-toxin I) is proposed for the RTX-toxin produced by the reference strains for serotypes 1, 5a, 5b, 9,10 and 11, which was previously named haemolysin I (HlyI) or cytolysin I (ClyI). This protein is strongly haemolytic and shows strong cytotoxic activity towards pig alveolar macrophages and neutrophils; it has an apparent molecular mass in the range 105 to 110 kDa. The genes of the operon will have the designations and for the activator, the structural gene and the two secretion genes respectively. The designation ApxII is proposed for the RTX-toxin which is produced by all serotype reference strains except serotype 10 and which was previously named App, HlyII. ClyII or Cyt. This protein is weakly haemolytic and moderately cytotoxic and has an apparent molecular mass between 103 and 105 kDa. The genes of the operon will have the designations for the activator gene and for the structural toxin gene. In the operon, no genes for secretion proteins have been found. Secretion of ApxII seems to occur via the products of the secretion genes and of the operon. The designation ApxIII is proposed for the non-haemolytic RTX-toxin of the reference strains for serotypes 2, 3, 4, 6 and 8, which was previously named cytolysin III (ClyIII), pleurotoxin (Ptx), or macrophage toxin (Mat). This protein is strongly cytotoxic and has an apparent molecular mass of 120 kDa. The genes of the operon have the designations and for the activator gene, the structural gene and the two secretion genes respectively.

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1993-08-01
2024-11-08
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References

  1. Anderson C., Potter A.A., Gerlach G.F. 1991; Isolation and molecular characterization of spontaneously occurring cytolysin- negative mutants of Actinobacillus pleuropneumoniae serotype 7.. Infection and Immunity 59:4110–4116
    [Google Scholar]
  2. Bhatia B., Mittal K.R., Frey J. 1991; Factors involved in immunity against Actinobacillus pleuropneumoniae in mice.. Veterinary Microbiology 29:147–158
    [Google Scholar]
  3. Van Den Bosch J.F. 1991; Actinobacillus pleuropneumoniae subunit vaccine.. Patent application EP-0453024.
    [Google Scholar]
  4. Van Den Bosch J.F., Jongenelen I.M.C.A., Pubben A.N.B., Van Vugt F.G.A., Segers R.P.A.M. 1992; Protection induced by a trivalent A. pleuropneumoniae subunit vaccine.. Proceedings 12th IPVS, The Hague, The Netherlands p. 194:
    [Google Scholar]
  5. Bosse J.T., Johnson R.P., Nemec M., Rosendal S. 1992; Protective local and systemic antibody responses of swine exposed to an aerosol of Actinobacillus pleuropneumoniae serotype 1.. Infection and Immunity 60:479–484
    [Google Scholar]
  6. Burrows L.L., Lo R.Y. 1992; Molecular characterization of an RTX determinant from Actinobacillus suis.. Infection and Immunity 60:2166–2173
    [Google Scholar]
  7. Chang Y.F., Young R., Struck D.K. 1989; Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.. DNA 8:635–647
    [Google Scholar]
  8. Chang Y.F., Young R.Y., Struck D.K. 1991; The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA and appBD loci flanked by pseudogenes.. Journal of Bacteriology 173:5151–5158
    [Google Scholar]
  9. Devenish J., Rosendal S. 1991; Calcium binds to and is required for biological activity of the 104-kilodalton hemolysin produced byActinobacillus pleuropneumoniae serotype 1.. Canadian Journal of Microbiology 37:317–321
    [Google Scholar]
  10. Devenish J., Rosendal S., Bosse J.T. 1990a; Humoral antibody response and protective immunity in swine following immunization with the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae.. Infection and Immunity 58:3829–3832
    [Google Scholar]
  11. Devenish J., Rosendal S., Bosse J.T., Wilkie B.N., Johnson R. 1990b; Prevalence of seroreactors to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae in swine herds.. Journal of Clinical Microbiology 28:789–791
    [Google Scholar]
  12. Devenish J., Brown J.E., Rosendal S. 1992; Association of the RTX proteins of Actinobacillus pleuropneumoniae with hemolytic, CAMP, and neutrophil-cytotoxic activities.. Infection and Immunity 60:2139–2142
    [Google Scholar]
  13. Dom P., Haesebrouck F., Kamp E.M., Smits M.A. 1992; Influence of Actinobacillus pleuropneumoniae serotype-2 and its cytolysins on porcine neutrophil chemiluminescence.. Infection and Immunity 60:4328–4334
    [Google Scholar]
  14. Fedorka Cray P.J., Huether M.J., Stine D.L., Anderson G.A. 1990; Efficacy of a cell extract from Actinobacillus (Haemophilus) pleuropneumoniae serotype 1 against disease in swine.. Infection and Immunity 58:358–365
    [Google Scholar]
  15. Felmlee T., Pellett S., Lee E.Y., Welch R.A. 1985; Escherichia coli hemolysin is released extracellularly without cleavage of a signal peptide.. Journal of Bacteriology 163:88–93
    [Google Scholar]
  16. Frey J., Nicolet J. 1988a; Recognition of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca2+.. Infection and Immunity 56:2570–2575
    [Google Scholar]
  17. Frey J., Nicolet J. 1988b; Purification and partial characterization of a hemolysin produced by Actinobacillus pleuro-pneumoniae type strain 4074.. FEMS Microbiology Letters 55:41–46
    [Google Scholar]
  18. Frey J., Nicolet J. 1990; Hemolysin patterns of Actinobacillus pleuropneumoniae.. Journal of Clinical Microbiology 28:232–236
    [Google Scholar]
  19. Frey J., Nicolet J. 1991; Immunological properties of Actinobacillus pleuropneumoniae hemolysin I.. Veterinary Microbiology 28:61–73
    [Google Scholar]
  20. Frey J., Deillon J.B., Gygi D., Nicolet J. 1991a; Identification and partial characterization of the hemolysin (HlyII) of Actinobacillus pleuropneumoniae serotype 2.. Veterinary Microbiology 28:303–312
    [Google Scholar]
  21. Frey J., Meier R., Gygi D., Nicolet J. 1991b; Nucleotide sequence of the hemolysin I gene from Actinobacillus pleuropneumoniae.. Infection and Immunity 59:3026–3032
    [Google Scholar]
  22. Frey J., Van Den Bosch H., Segers R., Nicolet J. 1992; Identification of a second hemolysin (HlyII) in Actinobacillus pleuropneumoniae serotype 1 and expression of the gene in Escherichia coli.. Infection and Immunity 60:1671–1676
    [Google Scholar]
  23. Frey J., Beck M., Stucki U., Nicolet J. 1993; Analysis of hemolysin operons in Actinobacillus pleuropneumoniae.. Gene 123:51–58
    [Google Scholar]
  24. Gygi D., Nicolet J., Frey J., Cross M., Koronakis V., Hughes C. 1990; Isolation of the Actinobacillus pleuropneumoniaehaemolysin gene and the activation and secretion of the prohaemolysin by the HlyC, HlyB and HlyD proteins of Escherichia coli.. Molecular Microbiology 4:123–128
    [Google Scholar]
  25. Gygi D., Nicolet J., Hughes C., Frey J. 1992; Functional analysis of the Ca2+-regulated hemolysin I operon of Actinobacillus pleuropneumoniae serotype 1.. Infection and Immunity 60:3059–3064
    [Google Scholar]
  26. Inzana T.J., Todd J., Ma J.N., Veit H. 1991; Characterization of a non-hemolytic mutant of Actinobacillus pleuropneumoniaeserotype 5: role of the 110 kilodalton hemolysin in virulence and immunoprotection.. Microbial Pathogenesis 10:281–296
    [Google Scholar]
  27. Issartel J.P., Koronakis V., Hughes C. 1991; Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation.. Nature; London: 351759–761
    [Google Scholar]
  28. Jansen R., Briaire J., Kamp E.M., Smits M.A. 1992a; The cytolysin genes of Actinobacillus pleuropneumoniae.. Proceedings 12th IPVS, The Hague, The Netherlands p. 197:
    [Google Scholar]
  29. Jansen R., Briaire J., Kamp E.M., Smits M.A. 1992b; Comparison of the cytolysin II genetic determinants of Actinobacillus pleuropneumoniae serotypes.. Infection and Immunity 60:630–636
    [Google Scholar]
  30. Jansen R., Briaire J., Kamp E.M., Gielkens A.L.J., Smits M.A. 1993; Cloning and characterization of the Actinobacillus pleuropneumoniae-RTX-toxin III (ApxIII) gene.. Infection and Immunity 61:947–954
    [Google Scholar]
  31. Kamp E.M., Van Leengoed L.A. 1989; Serotype-related differences in production and type of heat-labile hemolysin and heat-labile cytotoxin of Actinobacillus (Haemophilus) pleuropneumoniae.. Journal of Clinical Microbiology 27:1187–1191
    [Google Scholar]
  32. Kamp E.M., Popma J.K., Anakotta J., Smits M.A. 1991; Identification of hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae by use of monoclonal antibodies.. Infection and Immunity 59:3079–3085
    [Google Scholar]
  33. Lalonde G., Mcdonald T.V., Gardner P., ƠHanley P.D. 1989; Identification of a hemolysin from Actinobacillus pleuro-pneumoniae and characterization of its channel properties in planar phospholipid bilayers.. Journal of Biological Chemistry 264:13559–13564
    [Google Scholar]
  34. Van Leengoed L.A., Dickerson H.W. 1992; Influence of calcium on secretion and activity of the cytolysins of Actinobacillus pleuropneumoniae.. Infection and Immunity 60:353–359
    [Google Scholar]
  35. Ma J.N., Inzana T.J. 1990; Indirect enzyme-linked immunosorbent assay for detection of antibody to a 110000-molecular-weight hemolysin of Actinobacillus pleuropneumoniae.. Journal of Clinical Microbiology 28:1356–1361
    [Google Scholar]
  36. Macdonald J., Rycroft A.N. 1992; Molecular cloning and expression of ptxA, the gene encoding the 120-kilodalton cytotoxin of Actinobacillus pleuropneumoniae serotype 2.. Infection and Immunity 60:2726–2732
    [Google Scholar]
  37. Nicolet J. 1992; Actinobacillus pleuropneumoniae.. In Diseases of Swine, 7th Edn. pp. 401–408, Edited by. Leman A.D., Straw B.E., Mengeling W.L., D̛Allaire S., Taylor D.J. Ames, Iowa:: Iowa State University Press.;
    [Google Scholar]
  38. Rosendal S., Devenish J., Macinnes J.I., Lumsden J.H., Watson S., Xun H. 1988; Evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of Haemophilus (Actinobacillus) pleuropneumoniae.. American Journal of Veterinary Research 49:1053–1058
    [Google Scholar]
  39. Rossi Campos A., Anderson C., Gerlach G.F., Klashinsky S., Potter A.A., Willson P.J. 1992; Immunization of pigs against Actinobacillus pleuropneumoniae with two recombinant protein preparations.. Vaccine 10:512–518
    [Google Scholar]
  40. Rycroft A.N., Williams D., Cullen J.M., Macdonald J. 1991a; The cytotoxin of Actinobacillus pleuropneumoniae (pleuro-toxin) is distinct from the haemolysin and is associated with a 120 kDa polypeptide.. Journal of General Microbiology 137:561–568
    [Google Scholar]
  41. Rycroft A.N., Williams D., Mccandlish I.A., Taylor D.J. 1991b; Experimental reproduction of acute lesions of porcine pleuropneumonia with a haemolysin-deficient mutant of Actinobacillus pleuropneumoniae.. Veterinary Record 129:441–443
    [Google Scholar]
  42. Shope R.E. 1964; Porcine contagious pleuropneumonia. Experimental transmission, etiology and pathology.. Journal of Experimental Medicine 119:357–368
    [Google Scholar]
  43. Smits M.A., Bruire J., Jansen R., Smith H.E., Kamp E.M., Gielkens A.L. 1991; Cytolysins of Actinobacillus pleuro-pneumoniae serotype 9.. Infection and Immunity 59:4497–4504
    [Google Scholar]
  44. Stine D.L., Huether M.J., Moxley R.A., Srikumaran S. 1991; Actinobacillus pleuropneumoniae-induced thymic lesions in mice and pigs.. Infection and Immunity 59:2885–2891
    [Google Scholar]
  45. Udeze F.A., Kadis S. 1992; Effects of Actinobacillus pleuropneumoniae hemolysin on porcine neutrophil function.. Infection and Immunity 60:1558–1567
    [Google Scholar]
  46. Welch R.A. 1991; Pore-forming cytolysins of Gram-negative bacteria.. Molecular Microbiology 5:521–528
    [Google Scholar]
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