SUMMARY: Two sets of primers derived from genomic DNA libraries of serovars (strain RGA) and (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic species. The -derived primers (G1/G2) enabled amplification of DNA from and whereas the -derived primers (B64-I/B64-II) enabled the amplification of Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between and various other species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars and were detected in serum samples collected from patients during the first 10 days after the onset of illness.


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