Summary: A gene encoding glutamine synthetase (GS) was cloned on a recombinant plasmid pGS4 which enabled deletion mutants to utilize (NH)SO as a sole source of nitrogen. The nucleotide sequence of a 2423 bp DNA segment containing the GS-coding region of was determined and the complete amino acid sequence (701 residues) was deduced. Comparisons of the derived GS protein sequence with the amino acid sequences of GS from other bacteria indicate that it is the second reported example of a type III GS, originally identified in the obligate anaerobe The presence of GS in cells and the regulation of the cloned GS in cells was demonstrated by Western blot analysis.


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