Summary: Five yeast strains were isolated by enrichment culture on the basis of their ability to grow on mandelate and two of these strains were identified as In addition, a range of yeasts from culture collections was screened for growth on mandelate. The results suggest that mandelate utilization is a widespread but not universal characteristic within the genus Several of the yeasts contained an inducible NAD-dependent D(—)-mandelate dehydrogenase and an inducible dye-linked (presumably flavoprotein) L(+)-mandelate dehydrogenase. All the D(—)-mandelate dehydrogenases from the yeasts showed immunological cross-reactivity with each other (as judged by both immunoinhibition and immunoblotting), as did all the yeast L(+)-mandelate dehydrogenases that were tested. Determination of N-terminal amino acid sequences of several bacterial and yeast lactate and mandelate dehydrogenases, together with the evidence from the immunological studies, confirmed and extended previous proposals that there are several major groups of such dehydrogenases: FMN-dependent, membrane-bound L(+)-lactate and L(+)-mandelate dehydrogenases ( = approx. 44000) in bacteria, mitochondrial flavocytochrome L(+)-lactate and L(+)-mandelate dehydrogenases ( = approx. 59000) in yeasts, FAD-dependent, membrane-bound D(—)-lactate and D(—)-mandelate dehydrogenases in bacteria, and soluble NAD-dependent D(—)-mandelate dehydrogenases in both bacteria and yeasts.


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