Summary: Analysis of the region downstream of the gene of led to the detection of two open reading frames (ORFs). The deduced amino acid sequences of these ORFs were homologous to proteins encoded by genes not previously described and/or sequenced in Gram-positive bacteria. The deduced amino acid sequence of the first of these showed strong homology to the product of the gene of , which codes for a uracil phosphoribosyltransferase. The over-expression of the product of this gene appeared to be the source of the detrimental effect observed with phagemids carrying in hosts. The deduced amino acid sequence of the second ORF was homologous to the ClpP family of proteases. Examination of the upstream region of the gene led to the discovery of a new insertion sequence-like element which has been designated IS


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