1887

Abstract

Analysis of the region downstream of the gene of led to the detection of two open reading frames (ORFs). The deduced amino acid sequences of these ORFs were homologous to proteins encoded by genes not previously described and/or sequenced in Gram-positive bacteria. The deduced amino acid sequence of the first of these () showed strong homology to the product of the gene of , which codes for a uracil phosphoribosyltransferase. The over-expression of the product of this gene appeared to be the source of the detrimental effect observed with phagemids carrying in hosts. The deduced amino acid sequence of the second ORF () was homologous to the ClpP family of proteases. Examination of the upstream region of the gene led to the discovery of a new insertion sequence-like element which has been designated IS

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-5-913
1993-05-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/5/mic-139-5-913.html?itemId=/content/journal/micro/10.1099/00221287-139-5-913&mimeType=html&fmt=ahah

References

  1. Aduse-Opuku J., Gilpin M. L., Russell R. R. B. 1989; Genetic and antigenic comparison of Streptococcus mutans fructosyltransferase and glucan-binding protein. FEMS Microbiology Letters 50:279–282
    [Google Scholar]
  2. Dalrymple B. P., Caspers P., Arber W. 1984; Nucleotide sequence of the mobile genetic element IS30. EMBO Journal 3:2145–2149
    [Google Scholar]
  3. Dong Q., Sadouk A., Van Der Lelie D., Taghavi S., Erhat A., Nuyten J. M., Bosnrmans B., Mergeay M., Toussamt A. 1992; Cloning and sequencing of IS1086, an Alcaligenes eutrophus insertion element related to IS30 and IS4351. Journal of Bacteriology 174:8133–8138
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  5. Giffard P. M., Simpson C. L., Milward C. P., Jacques N. A. 1991; Molecular characterization of a cluster of at least two glucosyltransferase genes in Streptococcus Saliuarius ATCC 25975. Journal of General Microbiology 137:2577–2593
    [Google Scholar]
  6. Gottesman S. 1989; Genetics of proteolysis in Escherichia coli. Annual Review of Genetics 23:163–198
    [Google Scholar]
  7. Gottesman S., Squires C., Pichersky E., Carrington M. H., Hobbs K., Mattick J. S., Dalyrymple B., Kuramitsu H., Shiroza T., Foster T., Clark W. P., Ross B., Squires C. L., Maurizi M. A. 1990; Conservation of the regulatory subunit for the Clp ATP-dependent protease in procaryotes and eukaryotes. Proceedings of the National Academy of Sciences of the United States of America 87:3513–3517
    [Google Scholar]
  8. Gough J. A., Murray N. E. 1983; Sequence diversity among related genes for recognition of specific targets in DNA molecules. Journal of Molecular Biology 166:1–19
    [Google Scholar]
  9. Gray J. C., Hird S. M., Dyer T. A. 1990; Nucleotide sequence of the wheat chloroplast gene encoding the proteolytic subunit of an ATP-dependent protease. Plant Moletular Biology 15:947–950
    [Google Scholar]
  10. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Computer Applications in the Biosciences (CABIOS) 8:189–191
    [Google Scholar]
  11. Hiratsuka J., Shimada H., Whittier R., Ishibashi T., Sakamoto M., Mori M., Kondo C., Honji Y., Sun C. -R., Meng B. -Y., Li Y. -Q., Kanno A., Nishizawa Y., Hirai A., Shinozaki K., Sugiura M. 1989; The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Molecular and General Genetics 217:185–194
    [Google Scholar]
  12. Jacques N. A., Wittenberger C. L. 198l; Inactivation of cell-associated fructosyltransferase in Streptococcus salivarius. Journal of Bacteriology 148:912–918
    [Google Scholar]
  13. Kern L., de Montigny J., Jund R., Lacroute F. 1990; The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles. Gene 88:149–157
    [Google Scholar]
  14. Lewin B. 1990 Genes IV pp. 649–662 Oxford: Oxford University Press;
    [Google Scholar]
  15. Lockart P. J., Beanland T. J., Howe C. J., Larkum A. W. D. 1992; Sequence of Prochloron didemni atpDE and the inference of chloroplast origins. Proceedings of the National Academy of Sciences of the United States of America 89:2742–2746
    [Google Scholar]
  16. Maurizi M. A., Clark W. P., Katayama Y., Rudikoff S., Pumphrey J., Bowers B., Gottesman S. 1990a; Sequence and structure of ClpP, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli. Journal of Biological Chemistry 265:12536–12545
    [Google Scholar]
  17. Maurizi M. A., Clark W. P., Kim S. -H., Gottesman S. 1990b; ClpP represents a unique family of serine proteases. Journal of Biological Chemistry 265:12546–l2552
    [Google Scholar]
  18. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Milward C. P., Jacques N. A. 1990; Secretion of fructosyltransferase by Streptococcus salivarius involves the sucrose-de-pendent release of the cell-bound form. Journal of General Microbiology 136:165–169
    [Google Scholar]
  20. Murray N. E., Brammar W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants. Molecular and General Genetics 150:53–61
    [Google Scholar]
  21. Neuwald A. F., Stauffer G. V. 1990; IS30 activation of an smp′-lacZ gene fusion in Escherichia coli. FEMS Microbiology Letters 56:13–17
    [Google Scholar]
  22. Ohyama K., Fukuzawa H., Kohchi T., Shirai H., Sano T., Sano S., Umesono K., Shiki Y., Takeuchi M., Chang Z., Aota S., Inokuchi H., Ozeki H. 1986; Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature; London: 322572–574
    [Google Scholar]
  23. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences of the United States of America 85:2444–2448
    [Google Scholar]
  24. Pitty L. J., Giffard P. M., Gilpin M. L., Russell R. R. B., Jacques N. A. 1989; Cloning and expression of glycosyltransferase activities from Streptococcus salivarius. Journal of Dental Research 68:1681–1682
    [Google Scholar]
  25. Rasmussen U. B., Mygind B., Nygaard P. 1986; Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12. Biochimica et Biophysica Acta 881:268–275
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulsen A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  29. Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N., Matsubayashi T., Zaita N., Chunwongse J., Obokata J., Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B. Y., Sugita M., Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A., Tohdoh N., Shimada H., Sugiura M. 1986; The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO Journal 5:2043–2049
    [Google Scholar]
  30. Silhavy T. J., Barman M. L., Enquist L. W. 1984 Experiments with Gene Fusions Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Smith C. J. 1987; Nucleotide sequence analysis of Tn 455l: use of operon fusions to detect promoter activity in Bacteroides fragilis. Journal of Bacteriology 169:4589–4596
    [Google Scholar]
  32. Squires C., Squires C. L. 1992; The Clp proteins: proteolysis regulators or molecular chaperones. Journal of Bacteriology 174:1081–1085
    [Google Scholar]
  33. Walker G. J., Jacques N. A. 1987; Polysaccharides of oral streptococci. In Sugar Transport and Metabolism in Gram-positive Bacteria pp. 39–68 Reiser J., Peterkofsky A. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  34. Woese C. R. 1987; Bacterial evolution. Microbiological Reviews 51:221–271
    [Google Scholar]
  35. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-5-913
Loading
/content/journal/micro/10.1099/00221287-139-5-913
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error