1887

Abstract

Fortimicin-A (FTM-A; astromicin)-resistance genes ( genes) isolated from six producers of the FTM-group of antibiotics were analysed. These genes could be classified into two types by the resistance profiles to aminoglycoside antibiotics and by their DNA homologies. Three genes, from the istamycin producer ATCC 31603, from the sannamycin producer IFO 14239 and from the sporaricin producer ATCC 20501, conferred resistance to FTM-A, kanamycin (Km) and neomycin B (Nm-B), but not to gentamicin (Gm). The other three genes, from the FTM-A producer ATCC 21819, from the antibiotic SF-2052 producer sp. SF-2098 (ATCC 31580) and from the dactimicin producer ATCC 31570, conferred resistance to FTM-A, Km and Gm, but not to Nm-B. No DNA homology was detected between the two types of the resistance genes in Southern-blot analysis. The present results revealed that, in spite of the similarity of their biosynthesis genes, there are at least two different types of resistance genes among the FTM-group antibiotic producers.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-3-591
1993-03-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/3/mic-139-3-591.html?itemId=/content/journal/micro/10.1099/00221287-139-3-591&mimeType=html&fmt=ahah

References

  1. Beauclerk A. A. D., Cundliffe E. 1987; Sites of action of two ribosomal RNA methylases responsible for resistance to amino-glycosides. Journal of Molecular Biology 193:661–671
    [Google Scholar]
  2. Burnham M. K. R., Earl A. J., Bull J. H., Smith D. J., Turner G. 1989; DNA encoding ACV synthetase. European Patent Applications 88311655·0
    [Google Scholar]
  3. Chater K. F., Bruton C. J. 1985; Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO Journal 4:1893–1892
    [Google Scholar]
  4. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982; Gene cloning in Streptomyces. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  5. Cundliffe E. 1989; How antibiotic-producing organisms avoid suicide. Annual Review of Microbiology 43:207–233
    [Google Scholar]
  6. Dairi T., Hasegawa M. 1989; Common biosynthetic feature of fortimicin-group antibiotics. Journal of Antibiotics 42:934–943
    [Google Scholar]
  7. Dairi T., Ohta T., Hasegawa M. 1993; Organization and nature of fortimicin A (astromicin) biosynthetic genes studied by a cosmid library of Micromonospora olivasterospora. Molecular and General Genetics in the Press
    [Google Scholar]
  8. Deushi T., Iwasaki A., Kamiya K., Kunieda T., Mizoguchi T., Nakayama M., Itoh H., Mori T., Oda T. 1979; A new broad-spectrum aminoglycoside antibiotic complex sporaricin. I. Fermentation, isolation and characterization. Journal of Antibiotics 32:173–179
    [Google Scholar]
  9. Fishman S. E., Cox K., Larson J. L., Reynolds P. A., Seno E. T., Yeh W. K., Van Frank R., Hershberger C. L. 1987; Cloning genes for the biosynthesis of a macrolide antibiotic. Proceedings of the National Academy of Sciences of the United States of America 84:8248–8252
    [Google Scholar]
  10. Hintermann G., Crameri R., Vögtli M., Hütter R. 1984; Streptomycin-sensitivity in Streptomyces glauscesens is due to deletions comprising the structural gene coding for a specific phosphotransferase. Molecular and General Genetics 196:513–520
    [Google Scholar]
  11. Holmes D. J., Drocourt D., Tiraby G., Cundliffe E. 1991; Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius. Gene 102:19–26
    [Google Scholar]
  12. Hotta K., Takahashi A., Okami Y., Umezawa H. 1983; Relationship between antibiotic resistance and antibiotic productivity in actinomycetes which produce aminoglycoside antibiotics. Journal of Antibiotics 36:1789–1791
    [Google Scholar]
  13. Hotta K., Morioka M., Okami Y. 1989; Biosynthetic similarity between Streptomyces tenjimariensis and Micromonospora oliva-sterospora which produce fortimicin-group antibiotics. Journal of Antibiotics 42:745–751
    [Google Scholar]
  14. Inoue S., Ohba K., Shomura T., Kojima M., Tsuruoka T., Yoshida J., Kato N., Ito M., Amano S., Omoto S., Ezaki N., Ito T., Niida T. 1979; A novel aminoglycoside antibiotic, substance SF-2052. Journal of Antibiotics 32:1354–1356
    [Google Scholar]
  15. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibiotics in Streptomyces lividans. Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  16. Kelemen G. H., Cundliffe E., Finansek I. 1991; Cloning and characterization of gentamicin-resistance genes from Micro-monospora purpurea and Micromonospora rosea. Gene 98:53–60
    [Google Scholar]
  17. Kieser T. 1984; Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid 12:19–36
    [Google Scholar]
  18. Leskiw B. K., Aharonowitz Y., Mevarech M., Wolfe S., Vining L. C., Westlake D. W. S., Jensen S. E. 1988; Cloning and nucleotide sequence determination of the isopenicillin N synthetase gen from Streptomyces clavuligerus. Gene 62:187–196
    [Google Scholar]
  19. Malpartida F., Hopwood D. A. 1986; Physical and genetic characterization of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2). Molecular and General Genetics 205:66–73
    [Google Scholar]
  20. Malpartida F., Hallam S. E., Kieser H., Motamedi H., Hatchinson C. R., Butler M. J., Sugden D. A., Warren M., Mckillop C., Bailey C. R., Humphreys G. O., Hopwood D. A. 1987; Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic synthesis genes. Nature; London: 325818–821
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Matkovic B., Piendl W., Boeck A. 1984; Ribosomal resistance as a wide spread self-defense mechanism in aminoglycoside-producing Micromonospora species. FEMS Microbiology Letters 24:273–276
    [Google Scholar]
  23. Murakami T., Anzai H., Imai S., Satoh A., Nagaoka K., Thompson C. J. 1986; The bialaphos biosynthetic genes of Streptomyces hygroscopicus: molecular cloning and characterization of gene cluster. Molecular and General Genetics 205:42–50
    [Google Scholar]
  24. Nagano E., Hasegawa M., Kawamoto I. 1988; Novel cloning vectors. Japanese Patent Kokai 63:135031
    [Google Scholar]
  25. Nakano M., Mashiko H., Ogawara H. 1984; Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species. Journal of Bacteriology 157:79–83
    [Google Scholar]
  26. Nara T., Yamamoto M., Kawamoto I., Takayama L., Okachi R., Takasawa S., Sato T., Sato S. 1977; Fortimicins A and B, new aminoglycoside antibiotics. I. Producing organism, fermentation and biological properties of fortimicins. Journal of Antibiotics 30:532–540
    [Google Scholar]
  27. Odakura Y., Kase H., Itoh S., Satoh S., Takasawa T., Takahashi K., Shirahata K., Nakayama K. 1984; Bio-synthesis of astromicin and related antibiotics. II. Biosynthetic studies with blocked mutants of Micromonospora olivasterospora. Journal of Antibiotics 37:1670–1680
    [Google Scholar]
  28. Ohba K., Shomura T., Tsuruoka T., Kojima M., Inoue S., Itoh T. 1981; Production of antibiotic SF-2098. Japanese Patent Kokai56–18600
    [Google Scholar]
  29. Ohnuki T., Imanaka T., Aiba S. 1985; Self-cloning of an str gene cluster for streptomycin biosynthesis and streptomycin resistance. Journal of Bacteriology 164:85–94
    [Google Scholar]
  30. Ohta T., Hasegawa M. 1989; Cloning and characterization of the self-defense gene of S. tenjimariensis and its distribution. In Trends in Actinomycetology in Japan pp. 89–90 Koyama Y. Edited by Others Tokyo: Society for Actinomycetes;
    [Google Scholar]
  31. Ohta T., Hashimoto E., Hasegawa M. 1992; Cloning and characterization of sms13 encoding sannamycin glycyltransferase gene of Streptomyces sannanensis. Journal of Antibiotics 45:1167–1175
    [Google Scholar]
  32. Okami Y., Hotta K., Yoshida M., Ikeda D., Kondo S., Umezawa H. 1979; New aminoglycoside antibiotics, istamycins A and B. Journal of Antibiotics 32:964–966
    [Google Scholar]
  33. Piendl W., Boeck A. 1982; Ribosomal resistance in gentamicin producer organism Micromonospora purpurea. Antimicrobial Agents and Chemotherapy 22:231–236
    [Google Scholar]
  34. Piendl W., Boeck A., Cundliffe E. 1984; Involvement of 16S ribosomal RNA in resistance of the aminoglycoside-producers Streptomyces tenjimariensis, Streptomyces tenebrarius and Micromonospora purpurea. Molecular and General Genetics 197:24–29
    [Google Scholar]
  35. Reed K. C., Mann D. A. 1985; Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Research 13:7207–7220
    [Google Scholar]
  36. Rhodes P. M., Hunter I. S., Friend E. J., Warren M. 1984; Recombinant DNA methods for the oxytetracycline producer Streptomyces rimosus. Biochemical Society Transactions 12:586–587
    [Google Scholar]
  37. Schiffman D., Mevarech M., Jensen S. E., Cohen G., Aharo-Nowitz Y. 1988; Cloning and comparative analysis of the gene coding for isopenicillin N synthetase in Streptomyces. Molecular and General Genetics 214:562–569
    [Google Scholar]
  38. Skeggs P. A., Thompson J., Cundliffe E. 1985; Methylation of 16S ribosomal RNA and resistance to aminoglycoside antibiotics in clones of Streptomyces lividans carrying DNA from Streptomyces tenjimariensis. Molecular and General Genetics 200:415–421
    [Google Scholar]
  39. Skeggs P. A., Holmes D. J., Cundliffe E. 1987; Cloning of aminoglycoside-resistance determinants from Streptomyces tenebrarius and comparison with related genes from other actinomycetes. Journal of General Microbiology 133:915–923
    [Google Scholar]
  40. Stackebrandt E., Charfreitag O. 1990; Partial 16S rRNA primary structure of five Actinomyces species: phylogenetic implications and development of an Actinomyces israelii-specific oligonucleotide probe. Journal of General Microbiology 136:37–43
    [Google Scholar]
  41. Stanzak R., Matsushima P., Baltz R. H., Rao R. N. 1986; Cloning and expression in Streptomyces lividans of clustered erythromycin biosynthesis genes from Streptomyces erythreus. Bio/Technology 4:229–232
    [Google Scholar]
  42. Watanabe I., Deushi T., Yamaguchi T., Kamiya K., Nakayama M., Mori T. 1979; The structural elucidation of aminoglycoside antibiotics, sannamycins A and B. Journal of Antibiotics 32:1066–1068
    [Google Scholar]
  43. Weigel B., Burgett S. G., Chen V., Skatrud P. L., Frolik A., Queener S. W., Ingolia T. D. 1988; Cloning and expression of isopenicillin N synthetase genes from Streptomyces limpmanii and Aspergillus nidulans: prokaryotic homologies of an antibiotic biosynthetic gene. Journal of Bacteriology 170:3817–3826
    [Google Scholar]
  44. Yamamoto H., Hotta K, Okami Y., Umezawa H. 1981; Self-resistance of a Streptomyces which produces istamycins. Journal of Antibiotics 34:824–829
    [Google Scholar]
  45. Yamamoto H., Hotta K., Okami Y., Umezawa H. 1986; Ribosomal resistance of an istamycin producer, Streptomyces tenjimariensis,. to aminoglycoside antibiotics. Biochemical and Biophysical Research Communications 100:1396–1401
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-3-591
Loading
/content/journal/micro/10.1099/00221287-139-3-591
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error