1887

Abstract

The interaction between genes of conferring constitutive synthesis of isocitrate lyase ( and ) and fluoroacetate resistance () has been investigated. Although mutants are unable to induce the glyoxylate cycle enzyme isocitrate lyase in response to acetate as sole carbon source, this phenotype was suppressed in recombinants of the type . The and mutations do not alter significantly the activities of eight enzymes of intermediary metabolism assayed. We conclude that the genes are probably bona fide isocitrate lyase regulatory genes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-3-509
1993-03-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/3/mic-139-3-509.html?itemId=/content/journal/micro/10.1099/00221287-139-3-509&mimeType=html&fmt=ahah

References

  1. Apirion D. 1965; The two-way selection of mutants and revertants in respect of acetate utilisation and resistance to fluoroacetate in Aspergillus nidulans. Genetical Research 6:317–329
    [Google Scholar]
  2. Armitt S., Roberts C. F., Kornberg H. L. 1970; The role of isocitrate lyase in Aspergillus nidulans. FEBS Letters 7:231–234
    [Google Scholar]
  3. Armitt S., Roberts C. F., Kornberg H. L. 1971; Mutants of Aspergillus nidulans lacking malate synthase. FEBS Letters 12:276–278
    [Google Scholar]
  4. Armitt S., McCullough W., Roberts C. F. 1976; Analysis of acetate non-utilizing mutants in Aspergillus nidulans. Journal of General Microbiology 92:263–282
    [Google Scholar]
  5. Brice C. B., Kornberg H. L. 1968; Genetic control of isocitrate lyase in Escherichia coli. Journal of Bacteriology 96:2185–2186
    [Google Scholar]
  6. Clutterbuck A. J. 1984; Loci and linkage map of the filamentous fungus Aspergillus nidulans. In Genetic Maps 3 pp. 265–273 O’Brien S. J. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  7. Cooper T. G., Beevers H. 1969; Mitochondria and glyoxysomes from castor bean endosperm. Journal of Biological Chemistry 244:3507–3513
    [Google Scholar]
  8. Ellis H. A. 1959; A colorimetric method for the assay of soluble succinic dehydrogenase and pyridine nucleotide-linked dehydrogenases. Archives of Biochemistry and Biophysics 85:561–562
    [Google Scholar]
  9. Hill R. L., Bradshaw R. A. 1969; Fumarase. Methods in Enzymology 13:91–99
    [Google Scholar]
  10. Hynes M. J. 1977; Induction of the acetamidase of Aspergillus nidulans by acetate metabolism. Journal of Bacteriology 131:770–775
    [Google Scholar]
  11. Hynes M. J., Corrick C. M., Kelly J. M., Littlejohn T. G. 1988; Identification of the sites of action for regulatory genes controlling the amdS gene of Aspergillus nidulans. Molecular and Cell Biology 8:2589–2596
    [Google Scholar]
  12. Jones M. J., Lipmann F. 1955; Aceto-CoA-kinase. Methods in Enzymology 1:585–591
    [Google Scholar]
  13. Katz M. E., Hynes M. J. 1989; Isolation and analysis of the acetate regulatory gene facB from Aspergillus nidulans. Molecular and Cell Biology 9:5696–5701
    [Google Scholar]
  14. Khouw B. T., McCurdy H. D. 1969; Tricarboxylic acid cycle enzymes and morphogenesis in Blastocladiella emersonii. Journal of Bacteriology 99:197–205
    [Google Scholar]
  15. Kornberg A. 1955; Isocitrate dehydrogenase of yeast. Methods in Enzymology 1:705–707
    [Google Scholar]
  16. McCullough W., Roberts C. F. 1974; The role of malic enzyme in Aspergillus nidulans. FEBS Letters 41:238–242
    [Google Scholar]
  17. McCullough W., Roberts C. F. 1980; The genetic control of isocitrate lyase in Aspergillus nidulans. Journal of General Microbiology 120:67–84
    [Google Scholar]
  18. McCullough W., Payton M. A., Roberts C. F. 1977; Carbon metabolism in Aspergillus nidulans. In Genetics and Physiology of Aspergillus pp. 97–129 Smith J. A., Pateman J. A. Edited by London & New York: Academic Press;
    [Google Scholar]
  19. McCullough W., Roberts C. F., Osmani S. A., Scrutton M. C. 1986; Regulation of carbon metabolism in filamentous fungi. In Carbohydrate Metabolism in Cultured Cells pp. 287–355 Morgan M. J. Edited by New York: Plenum Press;
    [Google Scholar]
  20. Osmani S. A., Scrutton M. C. 1983; The sub-cellular localisation of pyruvate carboxylase and some other enzymes in Aspergillus ndulans. European Journal of Biochemistry 147:119–128
    [Google Scholar]
  21. Pontecorvo G., Roper J. A., Hemmons L. M., Macdonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
/content/journal/micro/10.1099/00221287-139-3-509
Loading
/content/journal/micro/10.1099/00221287-139-3-509
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error