1887

Abstract

Fermentative bacteria were isolated from refuse excavated from municipal solid waste landfills in New York, Florida and Arizona. Anaerobic bacteria cultured on enriched solid media ranged from 10 to 10 c.f.u. (g dry wt of refuse). A significant correlation ( <0·03) was found between numbers of anaerobes cultured at 37 °C and moisture content of refuse. Bacteria hydrolysing starch and protein represented 0-15 % of the total anaerobes cultured; no anaerobic bacteria hydrolysing ball-milled cellulose were isolated. Aerobic bacteria isolated on enriched medium ranged between 10 and 10 c.f.u. (g dry wt). Direct microscopic counts of total bacteria associated with refuse were in the order of 10 bacteria (g dry wt). These data suggest that, despite relatively high densities of bacteria in landfills, polymer hydrolysis is mediated by a small percentage of the fermentative population.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-2-387
1993-02-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/2/mic-139-2-387.html?itemId=/content/journal/micro/10.1099/00221287-139-2-387&mimeType=html&fmt=ahah

References

  1. Archer D.B., Peck M.W. 1989; The microbiology of methane production in landfills. In Microbiology of Extreme Environments and its Potential for Biotechnology pp. 187–204 Da Costa M.S., Duarte J.C., Williams R.A.D. Edited by London & New York: Elsevier Applied Science;
    [Google Scholar]
  2. Archer D.B., Robertson J.A. 1986; The fundamentals of landfill microbiology. In Energy from Landfill Gas pp. 116–122 Emberton J.R., Emberton R.F. Edited by Oxfordshire: Harwell Laboratory;
    [Google Scholar]
  3. Bagnara C., Toci R., Gaudin C., Belaich J.P. 1985; Isolation and characterization of a cellulolytic micro-organism Cellulomonas fermentans sp.nov. International Journal for Systematic Bacteriology 35:502–507
    [Google Scholar]
  4. Barlaz M.A., Milke M.W., Ham R.K. 1987; Gas production parameters in sanitary landfill simulators. Waste Management and Research 5:27–39
    [Google Scholar]
  5. Barlaz M.A., Ham R.K., Schaefer D.M. 1989a; Mass-balance analysis of anaerobically decomposed refuse. Journal of Environmental Engineering 115:1088–1102
    [Google Scholar]
  6. Barlaz M.A., Schaefer D.M., Ham R.K. 1989b; Bacterial population development and chemical characteristics of refuse decomposition in a simulated sanitary landfill. Applied and Environmental Microbiology 55:55–65
    [Google Scholar]
  7. Bookter T.J., Ham R.K. 1982; Stabilization of solid waste in landfills. Journal of Environmental Engineering Division, Proceedings of the American Society of Civil Engineers 1081089–1100
    [Google Scholar]
  8. Caldwell D.R., Bryant M.P. 1966; Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied Microbiology 14:794–801
    [Google Scholar]
  9. Campbell D.J.V., Fielding E.R., Archer D.B. 1985; Understanding refuse decomposition practices to improve landfill gas energy potential. In Proceedings of Energy from Biomass, Third EC Conference pp. 1151–1155 Palz W. Edited by London:: Elsevier Science Publishing.;
    [Google Scholar]
  10. Carra J.S., Cossu R. 1990 International Perspectives on Municipal Solid Wastes and Sanitary Landfilling San Diego: Academic Press;
    [Google Scholar]
  11. Colberg P.J. 1988; Anaerobic microbial degradation of cellulose, lignin, oligolignols, and monoaromatic lignin derivatives. In Biology of Anaerobic Microorganisms pp. 333–372 Edited by Zehnder A.J.B. New York:: John Wiley and Sons.;
    [Google Scholar]
  12. Cook H.A., Cromwell D.L., Wilson H.A. 1967; Microorganisms in household refuse and seepage water from sanitary landfills. Proceedings of the West Virginia Academy of Sciences 39107–114
    [Google Scholar]
  13. Donnelly J.A., Scarpino P.V. 1984; Isolation, characterization, and identification of micro-organisms from laboratory and full-scale landfills. EPA Project Summary, EPA600/S2-84–119 pp. 1–7 Cincinnati: Municipal Environmental Research Laboratory;
    [Google Scholar]
  14. Ellery W.N., Schleyer M.H. 1984; Comparison of homogenization and ultrasonication as techniques in extracting attached sedimentary bacteria. Marine Ecology-Progress Series 15:247–250
    [Google Scholar]
  15. Filip Z., Kuster E. 1979; Microbial activity and the turnover of organic matter in a municipal refuse disposed of in an landfill. European Journal of Applied Microbiology & Biotechnology 7:371–379
    [Google Scholar]
  16. Gerhardt P., Murray R.G.E., Costilow R.N., Nester E.W., Wood W.A., Krieg N.R., Phillips G.B. 1981 Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Grainger J.M., Jones K.L., Hotten P.M. 1984; Estimation and control of microbial activity in landfill. In Microbiological Methods for Environmental Biotechnology pp. 259–273 Grainger J.M., Lynch J.M. Edited by Orlando: Academic Press;
    [Google Scholar]
  18. Harper S.R., Pohland F.G. 1988; Design and management stategies for minimizing environmental impact at municipal solid waste landfill sites. Proceedings of Joint American Society of Civil Engineers-Canadian Society of Civil Engineers National Conference on Environmental Engineering, Vancouver pp. 669–688
    [Google Scholar]
  19. Hobbie J.E., Daley R.J., Jasper S. 1977; Use of Nuclepore filters for counting fluorescence microscopy. Applied and Environmental Microbiology 33:1225–1228
    [Google Scholar]
  20. Jones K.L., Grainger J.M. 1983; The application of enzyme activity measurements to a study of factors affecting protein, starch and cellulose fermentation in domestic refuse. European Journal of Applied Microbiology and Biotechnology 18:181–185
    [Google Scholar]
  21. Jones K.L., Rees J.F., Grainger J.M. 1983; Methane generation and microbial activity in a domestic refuse landfill site. European Journal of Applied Microbiology & Biotechnology 18:242–245
    [Google Scholar]
  22. Khan A.W., Patel G.B. 1990; Cellulose degradation by mesophilic anaerobic bacteria. In Synthesis and Biodegradation of Cellulose pp. 355–375 Haigler C.D., Weimer P.J. Edited by New York & Basel: Marcel Dekker;
    [Google Scholar]
  23. Leadbetter L.R., Foster J.W. 1960; Bacterial oxidation of gaseous alkanes. Archives of Microbiology 35:92–104
    [Google Scholar]
  24. Pahren H.R. 1987; Microorganisms in municipal solid waste and public health implications. CRC Critical Reviews in Environmental Control 17:187–228
    [Google Scholar]
  25. Parkes R.J., Senior E. 1988; Multistage chemostats and other models for studying anoxic ecosystems. In Handbook of Laboratory Model Systems for Microbial Ecosystems 1 pp. 51–71 Wimpenny J.W.T. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  26. Pohland F.G., Harper S.R., Chang K-C, Dertien J.T., Chian E.S.K. 1985; Leachate generation and control at landfill disposal sites. Water Pollution Research Journal of Canada 20:10–24
    [Google Scholar]
  27. Robson L.M., Chambliss G.H. 1989; Cellulases of bacterial origin. Enzyme and Microbial Technology 11:626–644
    [Google Scholar]
  28. Senior E., Balba M.T.M. 1990; Refuse decomposition. In Microbiology of Landfill Sites pp. 18–57 Senior E. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  29. Sleat R., Harries C., Viney I., Rees J.F. 1987; Activities and distribution of key microbial groups in landfill. Proceedings of the ISWA Symposium on Process, Technology and Environmental Impact of Sanitary Landfills, Cagliari, Sardinia pp. 1–14
    [Google Scholar]
  30. Stegmann R. 1983; New aspects on enhancing biological processes in sanitary landfill. Waste Management and Research 1:201–211
    [Google Scholar]
  31. Suflita J.M., Gerba C.P., Ham R.K., Palmisano A.C., Rathje W.L., Robinson J.A. 1992; Microbiological, chemical, and archaeological investigation of the Fresh Kills Landfill. Environmental Science and Technology 26:1486–1494
    [Google Scholar]
  32. United States Environmental Protection Agency 1990; Characterization of Municipal Solid Waste in the United States: 1990 Update. EPA Report no.530-SW-90-042 US Department of Commerce, National Technical Information Service;
  33. Velgyi M.I., Albright L.J. 1986; Microscopic enumeration of attached marine bacteria of seawater, marine sediment, fecal matter, and kelp blade samples following pyrophosphate and ultrasound treatments. Canadian Journal of Microbiology 32:121–126
    [Google Scholar]
  34. Westlake K. 1989; Cellulolytic bacteria in sanitary landfill. In Proceedings in Landfill Microbiology Research and Development Workshop pp. 20–33 Lawson P.S., Alston Y.R. Edited by Oxfordshire: Harwell Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-2-387
Loading
/content/journal/micro/10.1099/00221287-139-2-387
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error