1887

Abstract

A tryptophanase gene from SM-18 was cloned and sequenced. The structural gene for tryptophanase, , consisted of 1389 bp encoding 462 amino acid residues, and its nucleotide sequence and deduced amino acid sequence showed significant homology to those of from K12. A short open reading frame consisting of 31 amino acid residues was found upstream of tnaA, and it showed some similarity to the E. coli tnaC gene known to be a -acting regulatory element for transcription. A partial open reading frame homologous to the 5ʹ end of E. coli tnaB was observed at the 3ʹ-flanking region of tnaA. These genes may thus constitute an operon as in .

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1993-12-01
2021-07-27
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