Three additional alleles of the gene of , whose activity is required for spore outgrowth, were identified. The nucleotide sequence of three mutant genes was determined. Analyses of dominance-recessivity showed that the wild-type allele is dominant over the mutant ones. When the gene was placed under the control of the inducible promoter, the presence of IPTG was necessary to obtain normal growth. The results suggested that the gene is required for growth of . Expression of from the sporulation promoter negatively affected subsequent spore outgrowth, without altering vegetative growth and sporulation.


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