1887

Abstract

The cell cycle of was synchronized at low cell densities by growing cells under conditions of alternating light and dark periods. Synchronization of the cell cycle was demonstrated by determining cell size, cytokinesis, and release of daughter cells. The microscopic data were in agreement with the accumulation patterns of mRNAs for ribulose bisphosphate carboxylase/oxygenase small subunit, histone H4 and β-tubulin. Cells of secrete a sticky material which enables cells to adhere to a solid substrate during cell division. The secretion of this material was also cell-cycle-regulated and preceded cytokinesis. Recently, it was reported that in synchronous exponential-phase cultures of high cell densities, young daughter cells temporarily behave like gametes during the cell cycle. However, under our growth conditions this was not observed. First, daughter cells did not agglutinate with normal gametes of the opposite mating type. Second, extraction of growing cells did not release any agglutinin activity as measured both by an agglutination assay and by an agglutinin-specific monoclonal antibody. We conclude therefore that gametogenesis in is not necessarily a normal phase of the cell cycle.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-9-1941
1992-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/9/mic-138-9-1941.html?itemId=/content/journal/micro/10.1099/00221287-138-9-1941&mimeType=html&fmt=ahah

References

  1. Ares M., Howell S. H. 1982; Cell cycle stage-specific accumulation of mRN As encoding tubulin and other polypeptides in Chlamydomonas. Proceedings of the National Academy of Sciences of the United States of America 79:5577–5581
    [Google Scholar]
  2. Bernstein E. 1960; Synchronous division in Chlamydomonas moewusii. Science 131:1528–1529
    [Google Scholar]
  3. Demets R., Tomson A. M., Ran E. T. H., Sigon C. A. M., Stegwee,& van den Ende H. 1985; Synchronization of the cell divisioncycle of Chlamydomonas eugametos. Journal of General Microbiology 131:2919–2924
    [Google Scholar]
  4. Demets R., Tomson A. M., Stegwee D., van den Ende H. 1987; Control of the mating type competence rhythm in Chlamydomonas eugametos. Journal of General Microbiology 133:1081–1088
    [Google Scholar]
  5. Donnan L., Carvill E. P., Gilliland T. J., John P. C. L. 1985; The cell cycles of Chlamydomonas and Chlorella. New Phytologist 99:1–40
    [Google Scholar]
  6. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  7. Goldschmidt-Clermont M., Rahire M. 1986; Sequence, evolution, and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/ oxygenase in Chlamydomonas reinhardtii. Journal of Molecular Biology 191:421–432
    [Google Scholar]
  8. Harris E. H. 1989; The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use San Diego: Academic Press;
    [Google Scholar]
  9. Homan W., Musgrave A., De Nobel H., Wagter R., De Wit D., Kolk A., van den Ende H. 1988; Monoclonal antibodies directed against the sexual binding site of Chlamydomonas eugametos gametes. Journal of Cell Biology 107:177–189
    [Google Scholar]
  10. Hunnicutt G. R., Snell W. J. 1991; Rapid and slow mechanism for loss of cell adhesiveness during fertilization in Chlamydomonas. Developmental Biology 147:216–224
    [Google Scholar]
  11. Hunnicutt G. R., Kosfiszer M. G., Snell W. J. 1990; Cell body and flagellar agglutinins in Chlamydomonas reinhardtii: the cell body plasma membrane is a reservoir for agglutinins whose migration to the flagella is regulated by a functional barrier. Journal of Cell Biology 111:1605–1616
    [Google Scholar]
  12. Kates J. R., Jones R. F. 1964; The control of gametic differentiation in liquid cultures of Chlamydomonas. Journal of Cellular and Comparative Physiology 63:157–164
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lien T., Knutsen G. 1979; Synchronous growth of Chlamydomonas reinhardtii (Chlorophyceae): a review of optimal conditions. Journal of Phycology 15:191–200
    [Google Scholar]
  15. Marco Y., Rochaix J.-D. 1981; Comparison of the nuclear ribosomal units of five Chlamydomonas species. Chromosoma 81:629–640
    [Google Scholar]
  16. McAteer M., Donnan L., John P. C. L. 1985; The timing of division in Chlamydomonas. New Phytologist 99:41–56
    [Google Scholar]
  17. Musgrave A., van den Ende H. 1987; How Chlamydomonas court their partners. Trends in Biochemical Sciences 12:470–473
    [Google Scholar]
  18. Musgrave A., van Eijk E., te Welscher R., Broekman R., Lens P., Homan W. L., van den Ende H. 1981; Sexual agglutination factor from Chlamydomonas eugametos. Planta 153:362–369
    [Google Scholar]
  19. Musgrave A., De Wildt P., Broekman R., van den Ende H. 1983; The cell wall of Chlamydomonas eugametos. Immunological aspects. Planta 158:82–89
    [Google Scholar]
  20. Musgrave A., De Wildt P., van Etten I., Pijst H., Scholma C., Kooyman R., Homan W., van den Ende H. 1986; Evidence for a functional membrane barrier in the transition zone between the flagellum and cell body of Chlamydomonas eugametos gametes. Planta 167:544–553
    [Google Scholar]
  21. Nicholl D. S. T., Schloss J. A., John P. C. L. 1988; Tubulin gene expression in the Chlamydomonas reinhardtii cell cycle: elimination of environmentally induced artefacts and the measurement of tubulin mRNA levels. Journal of Cell Science 89:397–403
    [Google Scholar]
  22. Philipps G., Chaubet N., Chaboute M.-E., Ehling M., Gigot C. 1986; Genomic organization and nucleotide sequence of two com histone H4 genes. Gene 42:225–229
    [Google Scholar]
  23. PIjst H. L. A., Zilver R. J., Musgrave A., van den Ende H. 1983; Agglutination factor in the cell body of Chlamydomonas eugametos. Planta 158:403–409
    [Google Scholar]
  24. Sager R., Granick S. 1954; Nutritional control of sexuality in Chlamydomonas reinhardi. Journal of General Physiology 37:729–742
    [Google Scholar]
  25. Saito T., Tsubo Y., Matsuda Y. 1985; Synthesis and turnover of cell body-agglutinin as a pool of flagellar surface agglutinin in Chlamydomonas reinhardii gamete. Archives of Microbiology 142:207–210
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Samson M. R., Klis F. M., Homan W. L., van Egmond P., Musgrave A., van den Ende H. 1987; Composition and properties of the sexual agglutinins of the flagellated green alga Chlamydomonas eugametos. Planta 170:314–321
    [Google Scholar]
  28. Schloss J. A. 1990; A Chlamydomonas gene encodes a G protein β subunit-like polypeptide. Molecular and General Genetics 221:443–452
    [Google Scholar]
  29. Schmeisser E. T., Baumgartel D. M., Howell S. H. 1973; Gametic differentiation in Chlamydomonas reinhardi: cell cycle dependency and rates in attainment of mating competency. Developmental Biology 31:31–37
    [Google Scholar]
  30. Spudich J. L., Sager R. 1980; Regulation of the Chlamydomonas cell cycle by light and dark. Journal of Cell Biology 85:136–145
    [Google Scholar]
  31. Surzycki S. 1971; Synchronously grown cultures of Chlamydomonas reinhardi. Methods in Enzymology 23:67–73
    [Google Scholar]
  32. Tomson A. M., Demets R., Barker N. P. M., Stegwee D., van den Ende H. 1985; Gametogenesis in liquid cultures of Chlamydomonas eugametos. Journal of General Microbiology 131:1553–1560
    [Google Scholar]
  33. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  34. Versluis M., Klis F., M, Schuring F., van Egmond P., van den Ende H. 1989; The molecular basis of flagellar adhesion in Chlamydomonas. Plant Physiology 89:Suppl.1170
    [Google Scholar]
  35. Waffenschmidt S., Spessert R., Jaenicke L. 1988; Oligosaccharide side chains of wall molecules are essential for cell-wall lysis in Chlamydomonas reinhardtii. Planta 175:513–519
    [Google Scholar]
  36. Wiese L. 1965; On sexual agglutination and mating-type substances (gamones) in isogamous heterothallic chlamydomonads. I. Evidence of the identity of the gamones with the surface components responsible for the sexual flagellar contact. Journal of Phycology 1:46–54
    [Google Scholar]
  37. Youngblom J., Schloss J. A., Silflow C. D. 1984; The two β-tubulin genes of Chlamydomonas reinhardtii code for identical proteins. Molecular and Cellular Biology 4:2686–2696
    [Google Scholar]
  38. Zachleder V., Jakobs M., van den Ende H. 1991; Relationship between gametic differentiation and the cell cycle in the green alga Chlamydomonas eugametos. Journal of General Microbiology 137:1333–1339
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-9-1941
Loading
/content/journal/micro/10.1099/00221287-138-9-1941
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error