The gene encoding the 68 kDa protective outer-membrane protein of (P.68 pertactin) was cloned, sequenced and expressed in . The gene was isolated by DNA:DNA hybridization experiments using a radioactively-labelled fragment of the homologous gene from . DNA sequence analysis reveals that the gene is capable of encoding a protein with a molecular mass of 93996 Da (P.94); this prefnsor molecule is processed to form the P.68 antigen on the surface of . Heterologous expression of the full-length gene encoding P.94 in results in similar processing, with the P.68 antigen targeted to the bacterial outer membrane. Comparison of P.94 with the P.93 and P.95 prefnsors, encoding homologous proteins from and , shows a high degree (>90%) of homology. The major differences between all three proteins ocfn in the number of repeats of the two families (Gly-Gly-Xaa-Xaa-Pro) and (Pro-Gln-Pro) of reiterated sequence motifs.


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