1887

Abstract

The aim of this investigation was to perform an extensive screening using HPLC and C-NMR spectroscopy to disclose the spectrum of osmolytes produced by aerobic heterotrophic and anoxygenic phototrophic eubacteria. The most predominant solutes detected within a wide range of marine and halophilic micro-organisms were two recently discovered tetrahydropyrimidines ectoine and hydroxyectoine, which were synthesized in response to osmotic stress.

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1992-08-01
2021-04-22
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References

  1. Austin B. 1989; Novel pharmaceutical compounds from marine bacteria. Journal of Applied Bacteriology 67:461–470
    [Google Scholar]
  2. Avron M. 1986; The osmotic components of halotolerant algae. Trends in Biochemical Sciences 11:5–6
    [Google Scholar]
  3. Ben-Amotz A., Avron M. 1983; Accumulation of metabolites by halotolerant algae and its industrial potential. Annual Reviews in Microbiology 37:95–119
    [Google Scholar]
  4. Brown A. D. 1976; Microbial water stress. Bacteriological Reviews 40:803–846
    [Google Scholar]
  5. Caumette P., Baulaigue R., Matheron R. 1988; Characterization of Chromatium salexigens sp. nov., a halophilic Chromatiaceae isolated from mediterranean salinas. Systematic and Applied Microbiology 10:284–292
    [Google Scholar]
  6. Caumette P., Baulaigue R., Matheron R. 1991; Thiocapsa halophila sp. nov., a new halophilic purple sulfur bacterium. Archives of Microbiology 155:170–176
    [Google Scholar]
  7. Fendrich C. 1988; Halovibrio variabilis gen. nov. spec., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid eubacterium from Great Salt Lake, Utah, USA. Systematic and Applied Microbiology 11:36–43
    [Google Scholar]
  8. Galinski E. A. 1986 Salzadaptation durch kompatible Solute bei halophilen phototrophen Bakterien PhD Thesis University of Bonn;
    [Google Scholar]
  9. Galinski E. A. 1987; Halo-fermentation, a novel low water process for the production or organic chemicals and enzyme protective agents. In Bioreactors and Biotransformations pp. 201–212 Edited by Moody G. W., Baker P. B. London: Elsevier;
    [Google Scholar]
  10. Galinski E. A., Trüper H. G. 1982; Betaine, a compatible solute in the extremely halophilic phototrophic bacterium Ectothiorhodospira halochloris . FEMS Microbiology Letters 13:357–360
    [Google Scholar]
  11. Galinski E. A., Herzog R. M. 1990; The role of trehalose as a substitute for nitrogen-containing compatible solutes (Ectothiorhodospira halochloris). Archives of Microbiology 153:607–613
    [Google Scholar]
  12. Galinski E. A., Oren A. 1991; Isolation and structure determination of a novel compatible solute from the moderately halophilic purple sulfur bacterium Ectothiorhodospira marismortui . European Journal of Biochemistry 198:593–598
    [Google Scholar]
  13. Galinski E. A., Tindall B. J. 1991; Biotechnological prospects of halophilic and halotolerant microorganisms. In Molecular Biology and Biotechnology of Extremophiles pp. 76–114 Edited by Herbert R. A., Sharp R. J. Glasgow: Blackie;
    [Google Scholar]
  14. Galinski E. A., Pfeiffer H. P., Trüper H. G. 1985; 1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid – a novel cyclic amino acid from halophilic phototrophic bacteria of the genus Ectothiorhodospira . European Journal of Biochemistry 149:135–139
    [Google Scholar]
  15. Grothe S., Krogsrud R. L., McCellan D. J., Milner J. L., Wood J. M. 1986; Proline transport and osmotic stress response in Escherichia coli K-12. Journal of Bacteriology 166:253–259
    [Google Scholar]
  16. Imhoff J. F. 1986; Osmoregulation and compatible solutes in eubacteria. FEMS Microbiology Reviews 39:57–66
    [Google Scholar]
  17. Imhoff J. F., Trüper H. G. 1977; Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b. Archives of Microbiology 114:115–121
    [Google Scholar]
  18. Imhoff J. F., Rodriguez-Valera F. 1984; Betaine is the main compatible solute of halophilic eubacteria. Journal of Bacteriology 160:478–479
    [Google Scholar]
  19. Inbar L., Lapidot A. 1988; The structure and biosynthesis of new tetrahydropyrimidine derivatives in actinomycin D producer Streptomyces parvulus . Journal of Biological Chemistry 263:16014–16022
    [Google Scholar]
  20. Jovall P.-A., Tunblad-Johansson I., Adler L. 1990; 13C NMR analysis of production and accumulation of osmoregulatory metabolites in the salt-tolerant yeast Debaryomyces hansenii . Archives of Microbiology 154:209–214
    [Google Scholar]
  21. Kanemasa Y., Yoshioka T., Hayashi H. 1972; Alteration of the phospholipid composition of Staphylococcus aureus cultured in medium containing NaCl. Biochimica et Biophysica Acta 280:444–450
    [Google Scholar]
  22. Komaratat P., Kates M. 1975; The lipid composition of a halotolerant species of Staphylococcus epidermidis . Biochimica et Biophysica Acta 398:464–484
    [Google Scholar]
  23. Kubitschek H. E. 1987; Buoyant density variation during the cell cycle in microorganisms. CRC Critical Reviews in Microbiology 14:73–97
    [Google Scholar]
  24. Kushner D. J. 1988; What is the ‘true’ internal environment of halophilic and other bacteria?. Canadian Journal of Microbiology 34:482–486
    [Google Scholar]
  25. Kushner D. J., Kamekura M. 1988; Physiology of halophilic eubacteria. In Halophilic Bacteria Vol. 1 pp. 109–138 Edited by Rodriguez-Valera F. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  26. Lai M.-C., Sowers K. R., Robertson D. E., Roberts M. F., Gunsalus R. P. 1991; Distribution of compatible solutes in the halophilic methanogenic archaebacteria. Journal of Bacteriology 173:5352–5358
    [Google Scholar]
  27. Larsen P. I., Sydnes L. K., Landfald B., Strom A. R. 1987; Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Archives of Microbiology 147:1–7
    [Google Scholar]
  28. Le Rudulier D., Bernard T. 1986; Salt tolerance in Rhizobium: a possible role for betaines. FEMS Microbiology Reviews 39:67–72
    [Google Scholar]
  29. Lippert, Κ. & Galinski E. A. 1992; Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Applied Microbiology and Biotechnology 37:61–65
    [Google Scholar]
  30. Mackay M. A., Norton R. S., Borowitzka L. J. 1984; Organic osmoregulatory solutes in cyanobacteria. Journal of General Microbiology 130:2177–2191
    [Google Scholar]
  31. Müller E. 1991 Kompatible Solute und Prolingewinnung bei halophilen und halotoleranten Bacilli PhD Thesis University of Bonn;
    [Google Scholar]
  32. Okami Y. 1986; Marine microorganisms as a source of bioactive agents. Microbiological Ecology 12:65–79
    [Google Scholar]
  33. Oren A. 1985; Intracellular salt concentrations of the anaerobic halophilic eubacteria Haloanaerobium praevalens and Halobacteroides halobius . Canadian Journal of Microbiology 32:4–9
    [Google Scholar]
  34. Oren A., Kessel M., Stackebrandt E. 1989; Ectothiorhodospira marismortui sp. nov., an obligately anaerobic, moderately halophilic purple sulfur bacterium from a hypersaline sulfur spring on the shore of the Dead Sea. Archives of Microbiology 151:524–529
    [Google Scholar]
  35. Peters P., Galinski E. A., Trüper H. G. 1990; The biosynthesis of ectoine. FEMS Microbiology Letters 71:157–162
    [Google Scholar]
  36. Pollard A., Wyn Jones R. G. 1979; Enzyme activities in concentrated solutions of glycine betaine and other solutes. Planta 144:291–298
    [Google Scholar]
  37. Reed R. H., Borowitzka L. J., Mackay M. A., Chudek J. A., Foster R., Warr S. C. R., Moore D. J., Stewart W. D. P. 1986; Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiology Reviews 39:51–56
    [Google Scholar]
  38. Regev R., Peri I., Gilboa H., Avi-Dor Y. 1990; 13C NMR study of the interrelation between synthesis and uptake of compatible solutes in two moderately halophilic eubacteria. Archives of Biochemistry and Biophysics 277:106–112
    [Google Scholar]
  39. Rengpipat S., Lowe S. E., Zeikus J. G. 1988; Effect of extreme salt concentrations on the physiology and biochemistry of Halobacteroides acetoethylicus . Journal of Bacteriology 170:3065–3071
    [Google Scholar]
  40. Robertson D. E., Roberts M. F., Belay N., Stetter K. O., Boone D. R. 1990a; Occurrence of β-glutamate, a novel osmolyte, in marine methanogenic bacteria. Applied and Environmental Microbiology 56:1504–1508
    [Google Scholar]
  41. Robertson D. E., Noll D., Roberts M. F., Menaia J. A. G. F., Boone D. R. 1990b; Detection of the osmoregulator betaine in methanogens. Applied and Environmental Microbiology 56:563–565
    [Google Scholar]
  42. Rodriguez-Valera F. 1988 Halophilic Bacteria Vol. 1 Boca Raton, Florida: CRC Press;
    [Google Scholar]
  43. Severin J., Wohlfarth A., Galinski E. A. 1989; Evidence for new compatible solutes from halotolerant bacteria not capable of ectoine synthesis. Proceedings of the FEMS-NATO workshop on General and Applied Aspects of Halophilic Microorganisms17–22 September 1989Alicante, Spain
    [Google Scholar]
  44. Smith L. T., Smith G. M. 1989; An osmoregulated dipeptide in stressed Rhizobium meliloti . Journal of Bacteriology 171:4714–4717
    [Google Scholar]
  45. Smith L. T., Pocard J. A., Bernard T., Le Rudulier D. 1988; Osmotic control of glycine betaine biosynthesis and degredation in Rhizobium meliloti . Journal of Bacteriology 170:3142–3149
    [Google Scholar]
  46. Staley J. T., Stanley P. M. 1986; Potential commercial applications in aquatic microbiology. Microbiological Ecology 12:79–100
    [Google Scholar]
  47. Stock J. B., Rauch B., Roseman S. 1977; Periplasmic volume changes in Salmonella typhimurium and Escherichia coli . Journal of Biological Chemistry 252:7850–7861
    [Google Scholar]
  48. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
  49. Trüper H. G., Galinski E. A. 1986; Concentrated brines as habitats for micro-organisms. Experientia 42:1182–1187
    [Google Scholar]
  50. Trüper H. G., Galinski E. A. 1990; Biosynthesis and fate of compatible solutes in extremely halophilic phototrophic eubacteria. FEMS Microbiology Reviews 75:247–254
    [Google Scholar]
  51. Trüper H. G., Severin J., Wohlfarth A., Müller E., Galinski E. A. 1991; Halophily, taxonomy, phylogeny and nomenclature. In General and Applied Aspects of Halophilic Microorganisms NATO ASI Series A: Life Sciences vol. 201 pp. 3–7 Edited by Rodriguez-Valera F. New York: Plenum Press;
    [Google Scholar]
  52. Van Eck J. H., Prior B. A., Brandt E. V. 1989; Accumulation of polyhydroxy alcohols by Hansenula anomala in response to water stress. Journal of General Microbiology 135:3505–3513
    [Google Scholar]
  53. Ventosa A., Gutierrez M. C., Garcia M. T., Ruiz-Berraquero F. 1989; Classification of ‘Chromobacterium marismortui’ in a new genus Chromohalobacter gen. nov., as Chromohalobacter marismortui comb., nov., nom. rev . International Journal of Systematic Bacteriology 39:382–386
    [Google Scholar]
  54. Vreeland R. H. 1987; Mechanisms of halotolerance in micro-organisms. CRC Critical Reviews in Microbiology 14:311–356
    [Google Scholar]
  55. Wegmann K. 1986; Osmoregulation in eucaryotic algae. FEMS Microbiology Reviews 39:37–43
    [Google Scholar]
  56. Whatmore A. M., Chudek J. A., Reed R. H. 1990; The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis . Journal of General Microbiology 136:2527–2535
    [Google Scholar]
  57. Woese C. R. 1987; Bacterial evolution. Microbiology Reviews 51:222–271
    [Google Scholar]
  58. Wohlfarth A., Severin J., Galinski E. A. 1989; Screening for compatible solutes of halophilic eubacteria. In Microbiology of Extreme Environments and its Potential for Biotechnology p. 421 Proceedings of the Federation of European Microbiological Societies Symposium held in Troia;Portugal18–23 September 1988 Edited by da Costa M. S., Duarte J. C., Williams R. A. D. London: Elsevier Science Publishers;
    [Google Scholar]
  59. Wohlfarth A., Severin J., Galinski E. A. 1990; The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae . Journal of General Microbiology 136:705–712
    [Google Scholar]
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