1887

Abstract

The enzyme acetohydroxy acid synthase (AHS), which catalyses the first common step in the biosynthesis of isoleucine, leucine and valine, has been demonstrated to be present in in two isoenzymic forms. The complete nucleotide sequences of the genes and encoding these two enzymes have been determined. Sequence analysis revealed the presence of two open reading frames, of 1836 and 1737 nucleotides for and , respectively. The predicted amino acid sequences of the two isoenzymes, compared with the PCC 7942 AHS enzyme and the large subunits of the AHSI, II, III isoenzymes, revealed a notable degree of similarity. A small subunit has not been identified for either of the AHS isoenzymes. Analysis by Northern blot hybridization demonstrated that the and genes are transcribed to give mRNA species of approximately 2.15 kb and 1.95 kb, respectively.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1399
1992-07-01
2021-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1399.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1399&mimeType=html&fmt=ahah

References

  1. Barak Z., Calvo J. M., Schloss J. V. 1988; Acetolactate synthase isozyme III from Escherichia coli. Methods in Enzymology 166 455 458
    [Google Scholar]
  2. Chaleff R. S., Mauvais C. J. 1984; Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224 1443 1445
    [Google Scholar]
  3. De Felice M., Lago C. T., Squires C. M., Calvo J. M. 1982; Acetohydroxy acid synthase isoenzymes of Escherichia coli K12 and Salmonella typhimurium. Annales de Microbiologie 133A 251 256
    [Google Scholar]
  4. Eoyang L., Silverman P. M. 1988; Purification and assays of acetolactate synthase I from Escherichia coli K12. Methods in Enzymology 166 435 445
    [Google Scholar]
  5. Falco S. C., Dumas K. D. 1985; Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl. Genetics 109 21 35
    [Google Scholar]
  6. Falco S. C., Dumas K. D., Livak K. J. 1985; Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase. Nucleic Acids Research 13 4011 4027
    [Google Scholar]
  7. Forlani G., Riccardi G., De Rossi E., De Felice M. 1991; Biochemical evidence for multiple forms of acetohydroxy acid synthase in Spirulina platensis. Archives of Microbiology 155 298 302
    [Google Scholar]
  8. Friedberg D., Seijffers J. 1990; Molecular characterization of genes coding for wild-type and sulfonylurea-resistant acetolactate synthase in the cyanobacterium Synechococcus PCC7942. Zeitschrift für Naturforschung 45c 538 543
    [Google Scholar]
  9. Haughn G. W., Smith J., Mazur B., Somerville C. 1988; Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Molecular and General Genetics 21 266 271
    [Google Scholar]
  10. Hauser C. A., Hatfield G. W. 1983; Nucleotide sequence of the livB multivalent attenuator of Escherichia coli K12. Nucleic Acids Research 11 127 139
    [Google Scholar]
  11. Higgins D. G., Sharp P. M. 1989; Clustal: a package for performing multiple sequence alignments on a microcomputer. Gene 73 237 244
    [Google Scholar]
  12. Kozak M. 1983; Comparison of initiation of protein synthesis in Procaryotes, Eucaryotes, and organelles. Microbiological Reviews 47 1 45
    [Google Scholar]
  13. Lang B. F., Burger G. 1986; A collection of programs for nucleic acid and protein analysis, written in fortran 77 for IBM-PC compatible microcomputers. Nucleic Acids Research 14 455 465
    [Google Scholar]
  14. Lawther R. P., Hatfield G. W. 1980; Multivalent translation control of transcription termination at the attenuator of the ilvGEDA operon of Escherichia coli K-12. Proceedings of the National Academy of Sciences of the United States of America 77 1862 1866
    [Google Scholar]
  15. Lawther R. P., Calhoun D. M., Adams C. W., Hauser C. A., Gray J., Hatfield G. W. 1981; Molecular basis of valine resistance in Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 78 922 925
    [Google Scholar]
  16. Lee K. Y., Townsend J., Tepperwan J., Black M., Chui C. F., Mazur B., Dunsmuir P., Bedbrook J. 1988; The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO Journal 7 1241 1248
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning. A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Mazur B. J., Chui C. F., Smith J. K. 1987; Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides. Plant Physiology 85 1110 1117
    [Google Scholar]
  19. Ray T. B. 1984; Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiology 75 827 831
    [Google Scholar]
  20. Reddy K. J., Bullerjahn G. S., Sherman D. M., Sherman L. A. 1988; Cloning, nucleotide sequence, and mutagenesis of a gene (irpA) involved in iron-deficient growth of the cyanobacterium Synechococcus sp. strain PCC7942. Journal of Bacteriology 170 4466 4476
    [Google Scholar]
  21. Riccardi G., Sgra S., Ciferri O. 1981; Production of amino acids by analog resistant mutants of the cyanobacterium Spirulina platensis. Journal of Bacteriology 147 1002 1007
    [Google Scholar]
  22. Riccardi G., De Rossi E., Milano A. 1989; Amino acid biosynthesis and its regulation in cyanobacteria. Plant Science 63 135 151
    [Google Scholar]
  23. Riccardi G., Milano A., De Rossi E., Malfatti C., De Felice M. 1990; Amino acid biosynthesis in Spirulina. In Biosynthesis of Branched Chain Amino Acids pp. 193 197 Edited by Barak Z., Chipman D. M., Schloss J. V. Weinheim: Verlag Chemie and Balaban Publishers;
    [Google Scholar]
  24. Riccardi G., De Rossi E., Milano A., Forlani G., De Felice M. 1991; Molecular cloning and expression of Spirulina platensis acetohydroxy acid synthase genes in Escherichia coli. Archives of Microbiology 155 360 365
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74 5463 5467
    [Google Scholar]
  26. Schloss J. V., Van Dyk D. E. 1988; Acetolactate synthase isozyme from Salmonella typhimurium. Methods in Enzymology 166 445 454
    [Google Scholar]
  27. Shaner D. L., Anderson P. C., Stidham M. A. 1984; Imidazolinones: potent inhibitors of acetohydroxy acid synthase. Plant Physiology 76 545 546
    [Google Scholar]
  28. Shine J., Dalgarno L. 1974; The 3′ terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proceedings of the National Academy of Sciences of the United States of America 71 1342 1346
    [Google Scholar]
  29. Squires C. H., De Felice M., Devereux J., Calvo J. M. 1983; Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12. Nucleic Acids Research 11 5299 5313
    [Google Scholar]
  30. Tandeau de Marsac N., Houmard J. 1987; Advances in cyanobacterial molecular genetics. In The Cyanobacteria pp. 251 295 Edited by Fay P., Van Baalen C. Amsterdam: Elsevier;
    [Google Scholar]
  31. Tomioka N., Sugiura M. 1983; The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans. Molecular and General Genetics 191 46 50
    [Google Scholar]
  32. Umbarger H. 1983; The biosynthesis of isoleucine and valine and its regulation. In Amino Acids: Biosynthesis and Genetic Regulation pp. 245 265 Edited by Hermann K. M., Somerville R. L. London: Addison Wesley;
    [Google Scholar]
  33. Wek R. C., Hauser C. A., Hatfield G. W. 1985; The nucleotide sequence of the ilvBN operon of Escherichia coli: sequence homologies of the acetohydroxy acid synthase isozymes. Nucleic Acids Research 13 3995 4010
    [Google Scholar]
  34. Wiersma P. A., Schmiemann M. G., Condie J. A., Crosby W. L., Moloney M. M. 1989; Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus. Molecular and Generasl Genetics 219 413 420
    [Google Scholar]
  35. Yasui A., Takao M., Oikawa A., Kiener A., Walsh C. T., Eker A. P. M. 1988; Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans. Nucleic Acids Research 16 4447 4463
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-7-1399
Loading
/content/journal/micro/10.1099/00221287-138-7-1399
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error