1887

Abstract

Bacteriophage BFK20 was isolated from a strain that had become contaminated during industrial fermentation. BFK20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. The genome of this bacteriophage consists of a linear double stranded DNA molecule of 44–45 kb with cohesive ends. The capsid of phage BFK20 contains nine polypeptides with molecular masses from 22.0–108.0 kDa. BFK20 DNA was used as a donor for fragments carrying promoters and transcription-terminators.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1387
1992-07-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1387.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1387&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience Publishers;
    [Google Scholar]
  2. Barák I., Koptides M., Jucovič M., Šišova M., Timko J. 1990; Construction of a promoter-probe shuttle vector for Escherichia coli and brevibacteria. Gene 95 133 135
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7 1513 1523
    [Google Scholar]
  4. Bartone N., Blanco C. 1991; Improved vectors for transcriptional signal screening in corynebacteria. FEMS Microbiology Letters 84 97 102
    [Google Scholar]
  5. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 41 459 472
    [Google Scholar]
  6. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31 230 314
    [Google Scholar]
  7. Cabanes-Bastos E., Day G. A., Lichtenstein P. C. 1989; A sensitive and simple assay for neomycin phosphotransferase II activity in transgenic tissue. Gene 77 169 176
    [Google Scholar]
  8. Cadenas F. R., Martin F. J., Gil A. J. 1991; Construction and characterization of promoter-probe vectors for corynebacteria using the kanamycin-resistance reporter gene. Gene 98 117 121
    [Google Scholar]
  9. Gentz R., Langner A., Chang A., Cohen S., Bujard H. 1981; Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proceedings of the National Academy of Sciences of the United States of America 78 4936 4940
    [Google Scholar]
  10. Hongo M., Oki T., Ogata S. 1972; Phage contamination and control. In The Microbial Production of Amino Acids pp. 67 90 Edited by Yanada K., Kinoshita S., Tsunoda T., Aida K. Tokyo: Kodansha;
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227 680 685
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning, a Laboratory Manual Cold Spring Harbour, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Miwa K., Matsui K., Terabe M., Ito K., Ishida M., Takagi H., Nakamori S., Sano K. 1985; Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum. Gene 39 281 286
    [Google Scholar]
  14. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105 361 363
    [Google Scholar]
  15. Ozaki A., Katsumata R., Oka T., Furuya A. 1984; Transfection of Corynebacterium glutamicum with temperate phage OCG1. Agricultural and Biological Chemistry 48 2597 2601
    [Google Scholar]
  16. Pátek M., Ludvík J., Benada O., Hochmannová J., Nešvera J., Krumphanzl V., Bučko M. 1985; New bacteriophage-like particles in Corynebacterium glutamicum. Virology 140 360 363
    [Google Scholar]
  17. Rigby P. V., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113 237 251
    [Google Scholar]
  18. Santamaria R., Gil J. A., Mesas J. M., Martin J. F. 1984; Characterization of an endogenous plasmid and development of cloning vectors and transformation system in Brevibacterium lactofermentum. Journal of General Microbiology 130 2237 2246
    [Google Scholar]
  19. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98 503 517
    [Google Scholar]
  20. Taylor D. E., Brose E. C. 1988; Modified Bimboim–Doly method for rapid detection of plasmid copy number. Nucleic Acids Research 16 9056
    [Google Scholar]
  21. Trautwetter A., Blanco C. 1988; Isolation and preliminary characterization of twenty bacteriophages infecting either Brevibacterium or Arthrobacter strains. Applied and Environmental Microbiology 54 1466 1471
    [Google Scholar]
  22. Trautwetter A., Blanco C., Sicard A. Μ. 1987a; Structural characteristics of the Corynebacterium lilium bacteriophage CL31. Journal of Virology 61 1540 1545
    [Google Scholar]
  23. Trautwetter A., Blanco C., Bonnassie S. 1987b; Characterization of the corynebacteriophage CG33. Journal of General Microbiology 133 2945 2952
    [Google Scholar]
  24. Yamamoto K. R., Alberts B. M. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology 40 734 744
    [Google Scholar]
  25. Yeh P., Oreglia J., Prévôts F., Sicard A. M. 1986; Shuttle vector system for Brevibacterium lactofermentum. Gene 47 301 306
    [Google Scholar]
  26. Yoshinaga F., Nakamori S. 1983 Amino acids: Biosynthesis and Genetic Regulation Edited by Herman Κ. M., Somerville R. L. Reading, MA: Addison Wesley;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-138-7-1387
Loading
/content/journal/micro/10.1099/00221287-138-7-1387
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error