DNA from 17 strains of , including representatives of all the established DNA homology groups, was cleaved with RI or dIII and fragments were separated by agarose gel electrophoresis. Southern blots of this DNA were hybridized to a radioactively labelled DNA probe prepared from the cloned 16S ribosomal RNA operon of . Banding patterns of the chromosomal DNA digests and the autoradiograms were specific to DNA homology groups I (), IIA (mosquito-pathogenic strains), IIB () and V, but groups III and IV were not clearly distinguished. This suggests that the mosquito-pathogenic strains represent a separate subspecies.


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