The regulation of [production of hydrogen sulphide (HS)] in is complex. Previous studies have shown that expression is dependent upon the presence of reduced sulphur and anaerobiosis and is modulated by carbon source and growth stage. Transposon mutagenesis failed to find any potential -acting factors effective in the regulation of in relation to oxygen. Spontaneous mutants capable of expressing aerobically were isolated and characterized. These mutations are closely linked to and affect not only oxygen regulation but also the requirement for cyclic AMP and reduced sulphur. Analysis of merodiploid strains indicates that these mutations are -acting and that is not subject to autoregulation.


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