1887

Abstract

SUMMARY: Transposon Tn5, on a mobilizable ColE1 plasmid, on a Ti plasmid derepressed for bacterial transfer, and on the bacteriophage fd genome, was used to construct pathogenicity mutants of the fire blight pathogen . Eleven nonpathogenic mutants were isolated from 1600 independent mutants screened. These mutants were divided into three types: auxotrophs, exopolysaccharide (EPS)-deficient mutants and a mutant of the phenotype. According to their insertion sites the Tn5 mutants were mapped into several classes. Some of the mutants could be complemented with cosmid clones from a genomic library of the parent strain for EPS production on minimal agar. EPS-deficient mutants and the mutant could complement each other to produce virulence symptoms on pear slices.

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/content/journal/micro/10.1099/00221287-138-5-931
1992-05-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-5-931
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