Summary: The mechanism by which the aminoglycoside antibiotic streptomycin permeabilizes the cytoplasmic membrane of cells was reinvestigated. For this purpose, the extent of streptomycin-induced K loss from cells growing at low external K concentrations was taken as a measure of membrane permeabilization. Experiments with different K-uptake mutants showed that the antibiotic specifically increased the passive permeability of the cell membrane to K and other ions. These permeability changes were small and the membrane potential of the treated cells remained high. The membrane permeabilization was not due to a direct interaction of the antibiotic with the cell membrane, since cells that carry an mutation and synthesize proteins in a streptomycin-insensitive way did not lose K after the addition of the antibiotic. Due to misreading and premature termination of translation the cells synthesized aberrant proteins under the conditions where membrane permeabilization occurred. Two conditions are described under which the cells both degraded these mistranslated proteins rapidly and reaccumulated K, lending support to the hypothesis that membrane permeabilization is due to the presence of the mistranslated proteins in the cell membrane. Evidence is presented that the irreversibility of (dihydro)streptomycin uptake by cells washed free from the antibiotic might also be due to rapid degradation of the mistranslated proteins, leading to ‘caging’ of the antibiotic inside the cells.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error