SUMMARY: The relation between respiration and luminescence of was studied in the presence of the proton conductor CCCP (carbonyl cyanide -chlorophenylhydrazone). CCCP affected both luminescence and oxygen uptake rate, but did not alter the cellular ATP level. In the absence of CCCP, luminescence and oxygen uptake rate were almost constant over the pH range 6.5–8.5. However, at 10 μM-CCCP, both were apparently pH-dependent, with maxima observed at pH 8.5 and minima at pH 6.5. In the presence of CCCP (0.1–10 μM), the changes in the luminescence were in close correlation with those in the oxygen uptake rate regardless of the pH. HQNO (2-heptyl-4-hydroxyquinoline--oxide) and NaCN inhibited the CCCP-resistant luminescence. From these results, it is concluded that the CCCP-resistant luminescence is respiratory-dependent.


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