Motility of the alkalophilic sp. C-125, a flagellate bacterium, was demonstrated to be Na- and pH-dependent. Flagellin protein from this strain was purified to homogeneity and the N-terminal sequence determined. Using the gene of as a probe, the gene of sp. C-125 was identified and cloned into . Sequencing of this gene revealed that it encodes a protein of 272 amino acids ( 29995). The predicted N terminal sequence of this protein was identical to that determined by N-terminal sequencing of the flagellin protein from strain C-125. The alkalophilic sp. C-125 flagellin shares homology with other known flagellins in both the N- and C-terminal regions. The middle portion, however, shows considerable differences, even from that of flagellin from the related species, .


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