The gene , when present in high copy number, stimulates the expression of several extracellular protein genes during the onset of stationary phase, e.g. . A novel integration vector, pINT, was constructed for transcription expression studies; it employed a unique method of promoter insert production for fusion with the reporter gene. Deletions were made of the 5' flanking region of the promoter to localize the site responsible for SenS-mediated enhancement activity. pINT was used to translationally fuse promoter deletion fragments with the reporter gene. A site between −177 and −415 with respect to the start site of transcription was found to be required for the maximal SenS-mediated transcription increase from the promoter. A multicopy vector containing the coding region without its native negative regulation was highly unstable in ; this was due to the expressed insert.


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