1887

Abstract

Transcriptional fusions of DNA to the gene were screened for induction, initially by ethanol and then by hydrogen peroxide (HO). Two fusions were identified which were induced late following treatment with sublethal concentrations of HO (100 μM). The promoter was induced 4–5-fold and mapped to 11° while the promoter was induced 20-fold and mapped close to the right of the defective prophage PBSX, at about 120°. The fusion was induced by mitomycin C as well as HO, which correlated with the induction of PBSX by these agents. This was probably not a transcriptional induction, but rather a consequence of the induction of PBSX replication extending into adjacent regions of the chromosome.

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1992-10-01
2021-07-23
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References

  1. Anagnostopoulos C., & Spizizen J. 1961; Requirements for transformation in Bacillus subtilis . Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Ananthaswamy H. N., & Eisenstark A. 1977; Repair of hydrogen peroxide-induced single stranded breaks in Escherichia coli deoxyribonucleic acid. Journal of Bacteriology 130:187–191
    [Google Scholar]
  3. Bol D. K., & Yasbin R. E. 1990; Characterization of an inducible oxidative stress system in Bacillus subtilis . Journal of Bacteriology 172:3503–3506
    [Google Scholar]
  4. Carlsson J., & Carpenter V. S. 1980; The recA gene product is more important than catalase and superoxide dismutase in protecting Escherichia coli against hydrogen peroxide toxicity. Journal of Bacteriology 142:319–321
    [Google Scholar]
  5. Christman M. F., , Morgan R. W., , Jacobson F. S., & Ames B. N. 1985; Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium . Cell 41:753–762
    [Google Scholar]
  6. Deoonder R. A., , Lepesant J. A., , Lepesant-Kejzlarova J., , Billault A., , Steinmetz M., & Kunst F. 1977; Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Applied and Environmental Microbiology 33:989–993
    [Google Scholar]
  7. Demple B., , Johnson A., & Fung D. 1986; Exonuclease III and endonuclease IV remove 3′ blocks from DNA synthesis primers in H2O2-damaged Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 837731–7735
    [Google Scholar]
  8. Downs B. C. A., & Hoch J. A. 1991; Regulation of the oxidative stress response by the hpr gene in Bacillus subtilis . Journal of General Microbiology 137:1121–1125
    [Google Scholar]
  9. Downs B. C. A., , Murphy P., , Mcconnell D. J., & Devine K. M. 1987; Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis . Journal of Bacteriology 169:5771–5775
    [Google Scholar]
  10. Eisenstark A., & Perrot G. 1987; Catalase has only a minor role in protection against near-ultraviolet radiation damage in bacteria. Molecular and General Genetics 207:68–72
    [Google Scholar]
  11. Ferrari E., , Howard S. M., & Hoch J. A. 1986; Effect of stage 0 sporulation mutations on subtilisin expression. Journal of Bacteriology 166:173–179
    [Google Scholar]
  12. Hagensee M. E., , Bryan S. K., & Moses R. E. 1987; DNA polymerase III requirement for repair of DNA damage caused by methyl methanesulfonate and hydrogen peroxide. Journal of Bacteriology 169:4608–4613
    [Google Scholar]
  13. Hubacek J., & Glover S. W. 1970; Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli . Journal of Molecular Biology 50:111–127
    [Google Scholar]
  14. Imlay J. A., & Linn S. 1988; DNA damage and oxygen radical toxicity. Science 240:1302–1309
    [Google Scholar]
  15. Kogama T., , Farr S. B., , Joyce K. M., & Natvig D. O. 1988; Isolation of gene fusions (sol: :lacZ) inducible by oxidative stress in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 854799–4803
    [Google Scholar]
  16. Murphy P., , Downs B., , Mcconnell D. J., & Devine K. M. 1987; Oxidative stress and growth temperature in Bacillus subtilis . Journal of Bacteriology 169:5766–5770
    [Google Scholar]
  17. O'Kane C., , Cantwell B. A., & Mcconnell D. J. 1985; Mapping of the gene for endo-P-1,3-1,4-glucanase of Bacillus subtilis . FEMS Microbiology Letters 29:135–139
    [Google Scholar]
  18. O'Kane C., , Stephens M.A., & Mcconnell D. J. 1986; Integrable alpha-amylase plasmid for generating transcriptional fusions in Bacillus subtilis . Journal of Bacteriology 168:973–981
    [Google Scholar]
  19. Ortlepp S. A., , Ollington J. F., & Mcconnell D. J. 1983; Molecular cloning in Bacillus subtilis of a Bacillus licheniformis gene encoding a thermostable alpha-amylase. Gene 23:267–276
    [Google Scholar]
  20. Pergo M., & Hoch J. A. 1988; Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis . Journal of Bacteriology 170:2560–2567
    [Google Scholar]
  21. Sambrook J. Fritsch, , E. F., & Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Schaeffer P., , Millet J., & Aubert J.-P. 1965; Catabolic repression of bacterial sporulation. Proceedings of the National Academy of Sciences of the United States of America 54704–711
    [Google Scholar]
  23. Seaman E., , Tarmy E., & Marmur J. 1964; Inducible bacterio-phages of Bacillus subtilis . Biochemistry 3:607–613
    [Google Scholar]
  24. Stickler D. J., , Tucker R. G., & Kay D. 1965; Bacteriophage-like particles released from Bacillus subtilis after induction with hydrogen peroxide. Virology 26:142–145
    [Google Scholar]
  25. Storz G., , Tartaglia L. A., & Ames B. N. 1990; Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation. Science 248:189–194
    [Google Scholar]
  26. Thurm P., & Garro A. J. 1975; Isolation and characterization of prophage mutants of the defective Bacillus subtilis bacteriophage PBSX. Journal of Virology 16:184–191
    [Google Scholar]
  27. Warner F. D., , Kitos G. A., , Romano M. P., & Hemphill H. E. 1977; Characterization of SPβ a temperate bacteriophage from Bacillus subtilis 168M. Canadian Journal of Microbiology 23:45–51
    [Google Scholar]
  28. Wood H. E., , Dawson M. T., , Devine K., & Mcconnell D. J. 1990a; Characterization of PBSX, a defective prophage of Bacillus subtilis . Journal of Bacteriology 172:2667–2674
    [Google Scholar]
  29. Wood H. E., , Devine K. M., & Mcconnell D. J. 1990b; Characterization of a repressor gene (xre) and a temperature-sensitive allele from the Bacillus subtilis prophage, PBSX. Gene 96:83–88
    [Google Scholar]
  30. Yasbin R. E., , Fields P. I., & Andersen B. J. 1980; Properties of Bacillus subtilis 168 derivatives freed of their natural prophages. Gene 12:155–159
    [Google Scholar]
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