1887

Abstract

Summary: A mutant of defective in mycolic acid biosynthesis was isolated following chemical mutagenesis. Fatty acids were extracted from the mutant and subjected to structural analysis by thin-layer chromatography and high-performance liquid chromatography (HPLC) of both methyl and -bromophenacyl ester derivatives. Thin-layer chromatography did not show the presence of any fatty acid of comparable to that of standard methyl mycolate. The HPLC profile revealed a broad peak in the standard mycolic acid ester region. No characteristic peaks of mycolic acid esters comparable to the wild-type could be resolved. Mass spectral analysis of the HPLC-purified peak demonstrated the presence of shorter-chain fatty acids in the mutant. These data support the idea that the mutant accumulates precursors of mycolic acids and is incapable of carrying out the final conversion to mycolic acids of 60–90 carbon atoms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-9-2197
1991-09-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/9/mic-137-9-2197.html?itemId=/content/journal/micro/10.1099/00221287-137-9-2197&mimeType=html&fmt=ahah

References

  1. Bloch K., Vance D. 1977; Control mechanisms in the biosynthesis of saturated fatty acids. Annual Review of Biochemistry 46:263–298
    [Google Scholar]
  2. Butler W. R., Kilburn J. O. 1988; Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high performance liquid chromatography of their mycolic acids. Journal of Clinical Microbiology 26:50–53
    [Google Scholar]
  3. Butler W. R., Ahearn D. G., Kilburn J. O. 1986; High performance liquid chromatography of mycolic acids as a tool in the identification of Corynebacterium, Nocardia, Rhodococcus and Mycobacterium species. Journal of Clinical Microbiology 23:182–185
    [Google Scholar]
  4. David H. L. 1977; Alkaline phosphatases from Mycobacterium smegmatis. Journal of General Microbiology 101:99–102
    [Google Scholar]
  5. Gray G. R., Wong M. Y. H., Danielson S. J. 1982; The major mycolic acids of Mycobacterium smegmatis. Progress in Lipid Research 21:91–107
    [Google Scholar]
  6. Kaneda K., Imaizumi S., Mizuno S., Baba T., Tsukamura M., Yano I. 1988; Structure and molecular species composition of the three homologous series of α-mycolic acids from Mycobacterium spp. Journal of General Microbiology 134:2213–2229
    [Google Scholar]
  7. Kundu M., Basu J., Chakrabarti P. 1989; Purification and characterization of an extracellular lectin from Mycobacterium smegmatis. FEBS Letters 256:207–210
    [Google Scholar]
  8. Lacave C., Lanéelle M. A., Lanéelle G. 1990; Mycolic acid synthesis by Mycobacterium aurum cell free extracts. Biochimica et Biophysica Acta 1042:315–323
    [Google Scholar]
  9. Lanéelle M. A., Lacave C., Daffe M., Lanéelle G. 1988; Mycolic acids of Mycobacterium aurum, structure and biogenetic implications. European Journal of Biochemistry 177:631–635
    [Google Scholar]
  10. Matula M., Mitchell M., Elbein A. D. 1971; Partial purification and properties of a highly specific trehalose phosphate phosphatase from Mycobacterium smegmatis. Journal of Bacteriology 107:217–222
    [Google Scholar]
  11. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. Microbiological Classification and Identification198–250 Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  12. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid containing bacteria. Journal of Chromatography 188:221–233
    [Google Scholar]
  13. Qureshi N., Takayama K., Jordi H. C., Schnoes H. K. 1978; Characterization of the purification components of a new homologous series of α-mycolic acids from Mycobacterium tuberculosis H37RA. Journal of Biological Chemistry 253:5411–5417
    [Google Scholar]
  14. Ratledge C. 1982; Lipids: cell composition, fatty acid biosynthesis. The Biology of Mycobacteria 155–93 Ratledge C., Stanford J. London: Academic Press;
    [Google Scholar]
  15. Sathyamoorthy N., Takayama K. 1987; Purification and characterisation of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis. Journal of Biological Chemistry 262:13417–13423
    [Google Scholar]
  16. Sathyamoorthy N., Qureshi N., Takayama K. 1985; Purification and characterization of C28–55 fatty acids from Mycobacterium smegmatis. Canadian Journal of Microbiology 31:214–219
    [Google Scholar]
  17. Steck P. A., Schwartz B. A., Rosendahl M. S., Gray G. A. 1978; Mycolic acids. A reinvestigation. Journal of Biological Chemistry 253:5625–5629
    [Google Scholar]
  18. Takayama K., Qureshi N. 1984; Structure and synthesis of lipids. The Mycobacteria, a Source Book, part A315–344 Kubica G. P., Wayne L. G. New York: Marcel Dekker;
    [Google Scholar]
  19. Takayama K., Schaarves H. K., Armstrong E. L., Boyle R. W. 1975; Site of inhibitory action of isoniazid in the synthesis of mycolic acids in Mycobacterium tuberculosis. Journal of Lipid Research 16:308–317
    [Google Scholar]
  20. US Department of Health and Human Services 1980; Standard taxonomic tests. Mycobacterial Culture Collection (NIH Publication no. 80-289)220–239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-9-2197
Loading
/content/journal/micro/10.1099/00221287-137-9-2197
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error