1887

Abstract

Several strains of species of the fungal genus , and of , were screened for content of the polyunsaturated fatty acids (PUFAs) arachidonic acid (AA) and eicosapentaenoic acid (EPA). The aim of the investigation was to establish alternative sources of these PUFAs, which are of importance in human nutrition. As a relatively prolific producer of EPA and AA, strain # 144 was selected for a study of conditions that enhance their production over baseline levels that are present in the fungus when cultured for 6 d at 25°C with rotary shaking (120 r.p.m.) in Vogel’s medium containing sucrose as the carbon substrate. The levels of AA and EPA under these conditions were 133 ± 27 and 138 ± 25 mg l ( = 5), respectively. Maximal production of these fatty acids was accomplished by the following sequence of steps. (1) Incubate the cultures for 6 d after inoculation under the conditions described above. Then (2) add glucose to the cultures (2%, w/v, final concentration) and incubate for a further 6 d at 13°C. Under these conditions, the AA content of the mycelium was 205% higher than baseline levels and the EPA content was 198% higher. (3) Allow the cultures to remain stationary for 10 d which increases the AA content to 253% above baseline levels and the EPA content by 236%. Using such a procedure, 322 mg AA I and 383 mg EPA l were produced.

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1991-08-01
2024-12-13
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References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Bowman R. D., Mumma R. O. 1967; The lipids of Pythium ultimum. Biochimica et Biophysica Acta 144:501–510
    [Google Scholar]
  3. Behrens P. W., Hoeksema S. D., Arnett K. L., Cole M. S., Heubner T. A., Rutten J. M., Kyle D. J. 1989; Eicosa-pentaenoic acid in algae. Novel Microbial Products253–259 Demain A. L., Somkuti G. A., Hunter-Cevera J. C., Rossmoore H. W. Washington, DC: Society for Industrial Microbiology;
    [Google Scholar]
  4. Erwin J. 1973; Comparative biochemistry of fatty acids in eucaryotic microorganisms. Lipids and Biomembranes of Eucary-otic Microorganisms41–143 Erwin J. A. New York: Academic Press;
    [Google Scholar]
  5. Kerwin J. L., Duddles N. D. 1989; Reassessment of the roles of phospholipids in sexual reproduction by sterol-auxotrophic fungi. Journal of Bacteriology 171:3831–3839
    [Google Scholar]
  6. Nelson N. A., Kelly R. C., Johnson R. A. 1982; Prostaglandins and the arachidonic cascade. Chemical and Engineering News 60:1–15
    [Google Scholar]
  7. Radwan S. S., Soliman A. H. 1988; Arachidonic acid from fungi utilizing fatty acids with shorter chains as sole sources of carbon and energy. Journal of General Microbiology 134:387–393
    [Google Scholar]
  8. Ratledge C. 1986; Lipids. Biotechnology: a Comprehensive Treatise 4185–213 Pape H., Rehm H. J. Deerfield Beach, FL: VCH Publishers;
    [Google Scholar]
  9. Sanders T. A. B. 1988; Essential and trans-fatty acids in nutrition. Nutritional Research Review 1:57–78
    [Google Scholar]
  10. Shaw R. 1966; The polyunsaturated fatty acids of microorganisms. Advances in Lipid Research 4:107–174
    [Google Scholar]
  11. Shimizu S., Kawashima H., Shinmen Y., Akimoto K., Yamada H. 1988a; Production of eicosapentaenoic acid by Mortierella fungi. Journal of the American Oil Chemists’ Society 65:1455–1459
    [Google Scholar]
  12. Shimizu S., Shinmen H., Kawashima H., Akimoto K., Yamada H. 1988b; Fungal mycelia as a novel source of eicosapentaenoic acid. Biochemical and Biophysical Research Communications 150:335–341
    [Google Scholar]
  13. Shimizu S., Kawashima H., Akimoto K., Shinmen Y., Yamada H. 1989; Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid. Journal of the American Oil Chemists’ Society 66:342–347
    [Google Scholar]
  14. Shinmen Y., Shimizu S., Akimoto K., Kawashima H., Yamada H. 1989; Production of arachidonic acid by Mortierella fungi. Applied Microbiology and Biotechnology 31:11–16
    [Google Scholar]
  15. Simopoulos A. P. 1989; Summary of the NATO advanced research workshop on dietary ω-3 and ω-6 fatty acids: biological effects and nutritional essentiality. Journal of Nutrition 119:521–528
    [Google Scholar]
  16. Thompson G. A. 1985; Mechanisms of membrane response to environmental stress. Frontiers of Membrane Research in Agriculture347–357 St John J. B., Berlin E., Jackson P. C. Totowa, NJ: Rowman and Allanheld;
    [Google Scholar]
  17. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–445
    [Google Scholar]
  18. Weete J. D., Fuller M. S., Huang M. Q., Gandhi S. 1989; Fatty acids and sterols of selected Hyphochytriomycetes and Chytridiomycetes. Experimental Mycology 13:183–195
    [Google Scholar]
  19. Yamada H., Shimizu S., Shinmen Y. 1987a; Production of arachidonic acid by Mortierella elongata IS-5. Agricultural and Biological Chemistry 51:785–790
    [Google Scholar]
  20. Yamada H., Shimizu S., Shinmen Y., Kawashima H., Akimoto K. 1987b; Production of arachidonic acid and eicosapentaenoic acid by microorganisms. Journal of the American Oil Chemists’ Society 64:1254
    [Google Scholar]
  21. Yazawa K., Araki K., Okazaki N., Watanabe K., Ishikawa C, Inove A., Numao N., Kondo K. 1988; Production of eicosapentaenoic acid by marine bacteria. Journal of Biochemistry 103:5–7
    [Google Scholar]
  22. Yongmanitchai W., Ward O. P. 1989; Omega-3 fatty acids: alternative sources of production. Process Biochemistry 24:117–125
    [Google Scholar]
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