1887

Abstract

Membrane-bound chitin synthase from A3/5 had a pH optimum of 6·5, an apparent temperature optimum of 30 °C and an apparent for UDP--acetylglucosamine (UDP-GlcNAc) of 2·5 m. Digitonin-solubilized chitin synthase had a lower (about 25% of the original activity) and more variable activity than the membrane-bound chitin synthase from which it was prepared. The activity of solubilized chitin synthase was stabilized by incorporating Allosamidin (a chitinase inhibitor) into the reaction mixture, and an approximately 3·6-fold increase in activity was observed when the reaction mixture contained dimyristoylphosphatidylcholine. Concentrations of the organophosphorus fungicide edifenphos (Hinosan) up to 25 μ had no effect on the activity of membrane-bound chitin synthase, but between 25 and 145 μ, chitin synthase activity decreased with increase in fungicide concentration. Edifenphos caused non-competitive inhibition of chitin synthase activity with an apparent of 50 μ. Membrane-bound chitin synthase preparations from cultures of grown in 250 μ-edifenphos had a much lower activity than preparations from cultures grown in the absence of the fungicide. Pre-growth of in 250 μ (but not 80 μ) edifenphos also caused a reduction in incorporation of [H]GlcNAc into chitin (washed biomass was incubated in [H]GlcNAc-containing medium lacking fungicide).

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1991-03-01
2021-10-26
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