The operon, coding for γ-glutamyl kinase (GK) and γ-glutamyl phosphate reductase (GPR), was cloned from the chromosome of the wild-type strain of Sr41. From our sequence data the (1101 bp) and (1472 bp) genes were shown to code for two proteins of 39169 and 44640, respectively. Analysis of expression of the structural gene fused with the promoter showed that the operon is not subject to proline-mediated feedback repression. Amplification of the operon enabled us to determine GK activity, which was inhibited in the presence of a low concentration of l-proline. Comparison of the amino acid sequences of the GK and GPR proteins with those of the GK and GPR proteins from revealed extensive similarities.


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