1887

Abstract

produces an extracellular --fructofuranoside fructohydrolase (invertase) when grown on a medium containing the -fructofuranosides sucrose or raffinose, indicating that synthesis is subject to induction by the substrate. On a growth medium containing sucrose, production was maximal at 15 h in cultures incubated at 28 C°. After this time the level of detectable invertase in the cultures declined. A proportion of the enzyme was secreted during the linear growth phase of the fungus. Various sugars were investigated for induction of invertase, but only the two -fructofuranosides induced high production levels; with the other sugars, the enzyme was produced only at a low constitutive level. Mycelium grown under repressive conditions (1% glucose), rapidly produced invertase when transferred to sucrose-containing medium. After 80 min the invertase level in these cultures was 26-fold higher than the constitutive level. The repressive effect of other sugars, e.g. glucose and xylose, on invertase production was also demonstrated in this experimental system.

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1991-02-01
2021-03-02
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References

  1. Andres I., Peberdy J. F. 1974; The production of invertase in Aspergillus nidulans with reference to the effects of glucose and sucrose. Microbios 10:15–23
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Carlson M. 1984; A single SUC gene encodes both cytoplasmic and secreted invertase in Saccharomyces . Protein Transport and Secretion341–344
    [Google Scholar]
  4. Carlson M., Botstein D. 1982; Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase. Cell 28:145–154
    [Google Scholar]
  5. Carlson M., Osmond B. C., Botstein D. 1981; Mutants of yeast defective in sucrose utilization. Genetics 98:25–40
    [Google Scholar]
  6. Casanova M, Martinez J. P., Gil M. L., Sentandreu R., Ruiz-Herrera J. 1987; Different molecular forms of invertase in the slime variant of Neurospora crassa: comparison with the wild-type strain. Journal of General Microbiology 133:2447–2456
    [Google Scholar]
  7. Eilers F. I., Allen J., Hill E. P., Sussman A. S. 1964; Localization of disaccharidases in extracts of Neurospora after electrophoresis in polyacrylamide gels. Journal of Histochemistry and Cytochemistry 12:448–450
    [Google Scholar]
  8. Esmon P. C., Esmon B. E., Schauer I. E., Taylor A., Schekman R. 1987; Structure, assembly, and secretion of octameric invertase. Journal of Biological Chemistry 262:4387–4394
    [Google Scholar]
  9. Gabriel O., Wang S. F. 1969; Determination of enzymatic activity in polyacrylamide gels. I. Enzymes catalyzing the conversion of nonreducing substrates to reducing products. Analytical Biochemistry 27:545–555
    [Google Scholar]
  10. Galbraith J. C., Smith J. E. 1969; Changes in activity of certain enzymes of the tricarboxylic acid cycle and the glyoxylate cycle during the initiation of conidiation of Aspergillus niger . Canadian Journal of Microbiology 15:1207–1212
    [Google Scholar]
  11. Gascon S., Lampen O. 1968; Purification of internal invertase of yeast. Journal of Biological Chemistry 243:1567–1572
    [Google Scholar]
  12. Gascon S., Neumann N. P., Lampen O. 1968; Comparative study of the properties of the purified internal and external invertases from yeast. Journal of Biological Chemistry 243:1573–1577
    [Google Scholar]
  13. Gupta A. K., Bhatia I. S. 1982; Glucofructosan biosynthesis in Fusarium oxysporum: regulation and substrate specificity of fructosyl transferase and invertase. Phytochemistry 21:1249–1253
    [Google Scholar]
  14. Hackel R. A. 1975; Genetic control of invertase formation in Saccharomyces cerevisae I. Isolation and characterization of mutants affecting sucrose utilization. Molecular and General Genetics 140:361–370
    [Google Scholar]
  15. Hackel R. A., Khan N. A. 1978; Genetic control of invertase formation in Saccharomyces cerevisiae II. Isolation and characterization of mutants conferring invertase hyperproduction in strain EK-6B carrying the SUC3 Gene. Molecular and General Genetics 164:295–302
    [Google Scholar]
  16. Jacob F., Monod F. 1961; Genetic regulatory mechanisms in the synthesis of protein. Journal of Molecular Biology 3:318–356
    [Google Scholar]
  17. Kratky Z., Biely P., Bauer S. 1975; Mechanism of 2-deoxy-dglucose inhibition of cell-wall polysaccharide and glycoprotein biosynthesis in Saccharomyces cerevisiae . European Journal of Biochemistry 54:459–167
    [Google Scholar]
  18. Lampen J. O. 1968; External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  19. Meachum Z. D. Jr, Colvin H. J. Jr, Braymer H. D. 1971; Chemical and physical studies of Neurospora crassa invertase. Molecular weight, amino acid and carbohydrate composition and quaternary structure. Biochemistry 10:326–332
    [Google Scholar]
  20. Metzenberg R. L. 1962; A gene affecting the repression of invertase and trehalase in Neurospora . Archives of Biochemistry and Biophysics 96:468–474
    [Google Scholar]
  21. Metzenberg R. L. 1964; Enzymically active subunits of Neurospora invertase. Biochimica et Biophysica Acta 89:291–302
    [Google Scholar]
  22. Miller G. L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry 31:426–428
    [Google Scholar]
  23. Neuberg C., Mandl I. 1950; Invertase. In The Enzymes527–550 Sumner J. B., Myrback K. London & New York: Academic Press;
    [Google Scholar]
  24. Neumann N. P., Lampen O. 1967; Purification and properties of yeast invertase. Biochemistry 6:468–475
    [Google Scholar]
  25. Novick P., Novick F. S., Schekman R. 1981; Order of events in the yeast secretory pathway. Cell 25:461–469
    [Google Scholar]
  26. Olutiola P. O., Cole O. O. 1980; Extracellular invertase from Aspergillus flavus . Physiologia Plantarum 50:26–31
    [Google Scholar]
  27. Perlman D., Halvorson H. O. 1981; Distinct repressible mRNA for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell 25:525–536
    [Google Scholar]
  28. Perlman D., Halvorson H. O., Cannon L. E. 1982; Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence. Proceedings of the National Academy of Sciences of the United States of America 79781–785
    [Google Scholar]
  29. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  30. Raabo E., Terkildsen T. C. 1960; On the enzymatic determination of blood glucose. Scandinavian Journal of Clinical Laboratory Investigation 12:402–405
    [Google Scholar]
  31. Rodriguez L., Lampen J. O., Mackay V. L. 1981; SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase. Molecular and Cellular Biology 1:469–474
    [Google Scholar]
  32. Sargent M. L., Woodward D. O. 1969; Gene-enzyme relationships in Neurospora invertase. Journal of Bacteriology 97:867–872
    [Google Scholar]
  33. Sarokin L., Carlson M. 1986; Short repeated elements in the upstream regulatory region of the SUC2 gene of Saccharomyces cerevisiae . Molecular and Cellular Biology 6:2324–2333
    [Google Scholar]
  34. Trevithick J. R., Metzenberg R. L. 1966; Molecular sieving by Neurospora cell walls during secretion of invertase isoenzymes. Journal of Bacteriology 92:1010–1015
    [Google Scholar]
  35. Trimble R. B., Maley F., Chu F. K. 1983; Glycoprotein biosynthesis in yeast. Journal of Biological Chemistry 258:2562–2567
    [Google Scholar]
  36. Vainstein M. H. 1990; Regulation and characterisation of invertase in Aspergillus nidulans. PhD thesis University of Nottingham:
    [Google Scholar]
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