SUMMARY: The region of serogroup IIA has been cloned and expression of O antigen in K12 was demonstrated. Transposon mutagenesis analysis confined the DNA region required for O antigen expression to a 19·3 kb fragment, and the O antigen expressed was visualized by SDS-PAGE and silver staining. Southern hybridization analysis demonstrated significant levels of similarity between the region and the 3,6-dideoxyhexose pathway genes and , previously isolated from LT2, but no similarity to the abequose synthase gene of the same strain or the paratose synthase gene isolated from Ty2. The evolutionary relationship between the abequose biosynthetic genes of the two species of and is discussed.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error