1887

Abstract

The location of the genes coding for NAD independence in four unusual clinical isolates of was determined by transferring these genes to plasmid-free Rd by transformation and analysing transformants for the presence of plasmids by agarose gel electrophoresis. All NAD-independent transformants were found to carry a single plasmid species. The plasmids, originally harboured by the four isolates recovered from unrelated sources, were of the same size (5·25 kb). Spontaneous reversion to NAD dependence occurred with a low frequency (0·1 to 0·2% of the progeny of a single clone) in both and Rd. The revertants had lost this small plasmid. Mitomycin C exhibited a plasmid ‘curing’ effect with a frequency of‘curing’ of between 1 and 6% of the surviving clones. It was concluded that the genes conferring NAD independence were located on the small 5·25 kb plasmid.

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1991-10-01
2021-05-17
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