Strains of which have an auxotrophic requirement for lipoic acid were isolated by mutagenesis with the transposable element Mu J. The chromosomal location of these insertion mutations was determined to be at 14 map units by bacteriophage P22-mediated cotransduction. The gene is transcribed in the clockwise direction relative to the genetic map. Strains with ::lac operon fusions were used to characterize the transcriptional activity of the promoter. Transcription of the gene is not regulated by catabolite repression or lipoic acid concentration. The data indicate that the gene product is expressed constitutively at a low level.


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