1887

Abstract

The polar lipids and their fatty acid components in and Pseudomonas pickettii have been identified. In addition to diphosphatidylglycerol and phosphatidylglycerol (a trace only for ), all three species contained two forms of phosphatidylethanolamine differing in the presence or absence of α-hydroxy fatty acids. This seems to be a distinctive feature of species in RNA homology group II. Also, and (but not ) produced two forms of ornithine amide lipid, differing in the nature (hydroxy or non-hydroxy) of the ester-linked fatty acid. In all three species, the major non-hydroxy acids were hexadecanoic acid, a hexadecenoic acid, an octadecenoic acid, and cyclopropane derivatives of the monoenoic acids. The α-hydroxy acids were the derivatives of the same components, while the amide-linked acid of the ornithine amide lipids was mainly or entirely 3-hydroxyhexadecanoic acid. The possible taxonomic implications of the data are discussed.

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1991-01-01
2021-10-25
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References

  1. Bryn K., Jantzen E. 1982; Analysis of lipopolysaccharides by methanolysis, trifluoroacetylation, and gas chromatography on a fused-silica capillary column. Journal of Chromatography 240:405–413
    [Google Scholar]
  2. Byng G. S., Johnson J. L., Whitaker R. J., Gherna R. L., Jensen R. A. 1983; The evolutionary pattern of aromatic amino acid biosynthesis and the emerging phylogeny of pseudomonad bacteria. Journal of Molecular Evolution 19:272–282
    [Google Scholar]
  3. Chen M. M.-S. G., Chen P. H.-S. 1981; Identification of nonfermentative gram-negative bacilli by gas chromatography. II. Studies on cellular fatty acids. Proceedings of the National Science Council of Taiwan 5A:202–212
    [Google Scholar]
  4. Christenson J. C, Welch D. F., Mukwaya G., Muszynski M. J., Weaver R. E., Brenner D. J. 1989; Recovery of Pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis. Journal of Clinical Microbiology 27:270–273
    [Google Scholar]
  5. Cox A. D., Wilkinson S. G. 1989; Polar lipids and fatty acids of Pseudomonas cepacia . Biochimica et Biophysica Acta 1001:60–67
    [Google Scholar]
  6. Dees S. B., Hollis D. G., Weaver R. E., Moss C. W. 1983; Cellular fatty acid composition of Pseudomonas marginata and closely associated bacteria. Journal of Clinical Microbiology 18:1073–1078
    [Google Scholar]
  7. de Vos P., Goor M., Gillis M., de Ley J. 1985; Ribosomal ribonucleic acid cistron similarities of phytopathogenic Pseudomonas species. International Journal of Systematic Bacteriology 35:169–184
    [Google Scholar]
  8. de Vos P., Van Landschoot A., Segers P., Tytgat R., Gillis M., Bauwens M., Rossau R., Goor M., Pot B., Kersters K., Lizzaraga P., de Ley J. 1989; Genetic relationships and taxonomic localization of unclassified Pseudomonas and Pseudomonas-like strains by deoxyribonucleic acid : ribosomal ribonucleic acid hybridizations. International Journal of Systematic Bacteriology 39:35–9
    [Google Scholar]
  9. Drigues P., Lafforgue D., Asselineau J. 1981; Etude des phospholipides de Pseudomonas solanacearum Presence d'acides gras α-hydroxylés monoéthyléniques. Biochimica et Biophysica Acta 666:504–507
    [Google Scholar]
  10. Duthie A. H., Patton S. 1965; Purification of phospholipids recovered from thin-layer chromatograms for infrared spectral analysis. Journal of Lipid Research 6:320–322
    [Google Scholar]
  11. Fried A., Tietz A., Ben-Amotz A., Eichenberger W. 1982; Lipid composition of the halotolerant alga, Dunaliella bardami . Biochimica et Biophysica Acta 713:419–426
    [Google Scholar]
  12. Hara H., Cotter R. J. 1987; Assessment of the effect of growth temperature on the lipid composition of Serratia marcescens using laser desorption mass spectrometry. Rapid Communications in Mass Spectrometry 1:103–104
    [Google Scholar]
  13. Johnson J. L., Palleroni N. J. 1989; Deoxyribonucleic acid similarities among Pseudomonas species. International Journal of Systematic Bacteriology 39:230–235
    [Google Scholar]
  14. Kates M. 1986; Techniques of lipidology. Isolation, analysis and identification of lipids. In Laboratory Techniques in Biochemistry and Molecular Biology 3 Burdon R. H., van Knippenberg P. H. Amsterdam: Elsevier;
    [Google Scholar]
  15. Kawai Y., Yano I., Kaneda K., Yabuuchi E. 1988; Ornithine-containing lipids of some Pseudomonas species. European Journal of Biochemistry 175:633–641
    [Google Scholar]
  16. Kawanami J., Kimura A., Nakagawa Y., Otsuka H. 1969; Lipids of Streptomyces sioyaensis V. On the 2-hydroxy-13-methyl-tetradecanoic acid from phosphatidylethanolamine. Chemistry and Physics of Lipids 3:29–38
    [Google Scholar]
  17. Lanéelle M.-A., Promé D., Lanéelle G., Promé J.-C. 1990; Ornithine lipid of Mycobacterium tuberculosis: its distribution in some slow- and fast-growing mycobacteria. Journal of General Microbiology 136:773–778
    [Google Scholar]
  18. London E., Feigenson G. W. 1979; Phosphorus NMR analysis of phospholipids in detergents. Journal of Lipid Research 20:408–412
    [Google Scholar]
  19. Minnikin D. E., Abdolrahimzadeh H. 1974; The replacement of phosphatidylethanolamine and acidic phospholipids by an ornithine-amide lipid and a minor phosphorus-free lipid of Pseudomonas fluorescens NCMB 129. FEBS Letters 43:257–260
    [Google Scholar]
  20. Moss C. W. 1978; New methodology for identification of non-fermenters: gas-liquid chromatographic chemotaxonomy. In Glucose Nonfermenting Gram-Negative Bacteria in Clinical Microbiology171–201 Gilardi G. L. West Palm Beach: CRC Press;
    [Google Scholar]
  21. Moule A. L., Wilkinson S. G. 1987; Polar lipids, fatty acids, and isoprenoid quinones of Alteromonas putrefaciens (Shewanella putre-faciens). Systematic and Applied Microbiology 9:192–198
    [Google Scholar]
  22. Oyaizu H., Komagata K. 1983; Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. Journal of General and Applied Microbiology 29:17–40
    [Google Scholar]
  23. Okuyama H., Sasaki S. 1986; Two groups of phosphatidylethanolamine in Vibrio sp. strain ABE-1 separated by silica gel thin-layer chromatography. Journal of General and Applied Microbiology 32:483–89
    [Google Scholar]
  24. Palleroni N. J. 1984; Pseudomonas . In Bergey’s Manual of Systematic Bacteriology 1141–199 Krieg N. R., Holt J. G. Baltimore: Williams Wilkins;
    [Google Scholar]
  25. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas . International Journal of Systematic Bacteriology 23:333–339
    [Google Scholar]
  26. Pramanik B. N., Zechman J. M., Das P. R., Bartner P. L. 1990; Bacterial phospholipid analysis by fast atom bombardment mass spectrometry. Biomedical and Environmental Mass Spectrometry 19:164–170
    [Google Scholar]
  27. Ratledge C., Wilkinson S. G. 1988 Microbial Lipids 1 London: Academic Press;
    [Google Scholar]
  28. Sneath P. H. A., Stevens M., Sackin M. J. 1981; Numerical taxonomy of Pseudomonas based on published records of substrate utilization. Antonie van Leeuwenhoek 47:423–448
    [Google Scholar]
  29. Thiele O. W., Busse D., Schwinn G. 1971; Phosphatide der Brucellen. Zeitschrift fur Allgemeine Mikrobiologie 11:249–254
    [Google Scholar]
  30. Veys A., Callewaert W., Waelkens E., Van den Abbeele K. 1989; Application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens. Journal of Clinical Microbiology 27:1538–1542
    [Google Scholar]
  31. Wilkinson S. G. 1972; Composition and structure of the ornithine-containing lipid from Pseudomonas rubescens . Biochimica et Biophysica Ada 270:1–17
    [Google Scholar]
  32. Wilkinson S. G. 1988; Gram-negative bacteria. In Microbial Lipids 1299–488 Ratledge C., Wilkinson S. G. London: Academic Press;
    [Google Scholar]
  33. Wilkinson S. G., Caudwell P. F. 1980; Lipid composition and chemotaxonomy of Pseudomonas putrefaciens (Alteromonas putre-faciens). Journal of General Microbiology 118:329–341
    [Google Scholar]
  34. Woese C. R. 1987; Bacterial evolution. Microbiological Reviews 51:221–271
    [Google Scholar]
  35. Woese C. R., Blanz P., Hahn C. M. 1984; What isn’t a pseudomonad : the importance of nomenclature in bacterial classification. Systematic and Applied Microbiology 5:179–195
    [Google Scholar]
  36. Yamanaka S., Fudo R., Kawaguchi A., Komagata K. 1988; Taxonomic significance of hydroxy fatty acids in myxobacteria with special reference to 2-hydroxy fatty acids in phospholipids. Journal of General and Applied Microbiology 34:57–66
    [Google Scholar]
  37. Yano I., Furukawa Y., Kusunose M. 1970; αHydroxy fatty acid-containing phospholipids of Nocardia leishmanii . Biochimica et Biophysica Acta 202:189–191
    [Google Scholar]
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