1887

Abstract

Salt-induced change in protein synthesis were investigated in the cyanobacterium sp. PCC 6803. Immediately after a salt shock of 684 m-NaCl, total protein synthesis was almost completely blocked. Then, parallel to the accumulation of the osmoprotective compound glucosylglycerol, protein synthesis recovered gradually but remained diminished. The activation of glucosylglycerol synthesis was not inhibited by chloramphenicol at concentrations which totally inhibited protein synthesis. The qualitative protein composition of salt-shocked and control cells was similar. However, the rates of synthesis of single proteins were altered in cells shocked for 10 h and adapted to high salt conditions. Using two-dimensional gel electrophoresis, proteins were found which were synthesized at enhanced rates after adding salt.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1393
1990-07-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1393.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1393&mimeType=html&fmt=ahah

References

  1. Allen M.B., Arnon D.I. 1955; Studies on nitrogen-fixing blue- green algae. II. The sodium requirement of Anabaena cylindrica. . PhysiologiaPlantarum 8:653–660
    [Google Scholar]
  2. Apte S.K., Bhagwat A.A. 1989; Salinity-stress-induced proteins in two nitrogen-fixing Anabaena strains differentially tolerant to salt. Journal of Bacteriology 171:909–915
    [Google Scholar]
  3. Borbely G., Suranyi G., Korcz A., Palfi Z. 1985; Effect of heat shock on protein synthesis in the cyanobacteriumSynechococcussp. strain PCC 6301. Journal of Bacteriology 161:1125–1130
    [Google Scholar]
  4. Clark D., Parker J. 1984; Proteins induced by high osmotic pressure in Escherichia coli. . FEMS Microbiology Letters 25:81–83
    [Google Scholar]
  5. Hagemann M., Erdmann N., Wittenburg E. 1987; Synthesis of glucosylglycerol in salt-stressed cells of the cyanobacteriumMicrocystis firma. . Archives of Microbiology 148:275–279
    [Google Scholar]
  6. Hagemann M., Erdmann N., Wittenburg E. 1989; Studies concerning enzyme activities in salt-loaded cells of the cyanobacteriumMicrocystis firma. . Biochemie und Physiologie der Pflanzen 184:87–94
    [Google Scholar]
  7. Hecker M., Heim C., Völker U., Wölfel L. 1988; Induction of stress proteins by sodium chloride treatment in Bacillus subtilis. . Archives of Microbiology 150:564–566
    [Google Scholar]
  8. Hurkman W.J., Tanaka C.K. 1988; Polypeptide changes induced by salt stress, water deficit, and osmotic stress in barley roots: a comparison using two-dimensional gel electrophoresis. Electrophoresis 9:781–788
    [Google Scholar]
  9. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Molitor V., Erber W., Peschek G.A. 1986; Increased levels of cytochrome oxidase and sodium-proton antiporter in the plasma membrane of Anacystisnidulans after growth in sodium-enriched media. FEBS Letters 204:251–256
    [Google Scholar]
  11. O’farrell P.H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  12. Paleg L.G., Stewart G.R., Bradbeer J.W. 1984; Proline and glycine betaine influence protein solvation. Plant Physiology 75:974–978
    [Google Scholar]
  13. Potts M. 1986; The protein index of Nostoc commune UTEX 584 (cyanobacteria): changes induced in immobilized cells by water stress. Archives of Microbiology 146:87–95
    [Google Scholar]
  14. Reed R.H., Stewart W.D.P. 1985; Osmotic adjustment and organic solute accumulation in unicellular cyanobacteria from freshwater and marine habitats. Marine Biology 88:1–9
    [Google Scholar]
  15. Reed R.H., Richardson D.L., Stewart W.D.P. 1985; Na+ uptake and extrusion in the cyanobacteriumSynechocystis PCC 6714 in response to hypersaline treatment. Evidence for transient changes in plasmalemma Na+ permeability. Biochimica et BiophysicaActa 814:347–355
    [Google Scholar]
  16. Reed R.H., Borowitzka L.J., Mackay M.A., Chudeck J.A., Foster R., Warr S.R.C., Moore D.J., Stewart W.D.P. 1986; Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiology Reviews 39:51–56
    [Google Scholar]
  17. Sadka A., Lers A., Zamir A., Avron M. 1989; A critical examination of the role of de novo protein synthesis in the osmotic adaptation of the halotolerant alga Dunaliella. . FEBS Letters 244:93–98
    [Google Scholar]
  18. Schiewer U., Jonas L. 1977; Die WirkungunterschiedlicherNaCl-Konzentrationen auf die Ultrastruktur von Blaualgen. I. Microcystis firma. . Archiv fur Protistenkunde 119:127–145
    [Google Scholar]
  19. Warr S.R.C., Reed R.H., Stewart W.D.P. 1984; Osmotic adjustment of cyanobacteria: the effects of NaCl, KC1, sucrose and glycine betaine on glutamine synthetase activity in a marine and a halotolerant strain. Journal of General Microbiology 130:2169–2175
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1393
Loading
/content/journal/micro/10.1099/00221287-136-7-1393
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error