1887

Abstract

A glycerol-kinase-deficient mutant of was isolated. Genetic analysis revealed that the mutation is located on linkage group VI. The phenotype of this mutant differed from that of a glycerol kinase mutant of in its ability to utilize dihydroxyacetone (DHA). The weak growth on glycerol of the glycerol kinase mutant showed that glycerol phosphorylation is an important step in glycerol catabolism. The mutant could still grow normally on DHA because of the presence of a DHA kinase. This enzyme, probably in combination with an NAD-dependent glycerol dehydrogenase, present only in the mutant, is responsible for the weak growth of the mutant on glycerol. Enzymic analysis of both the mutant and the parental strain showed that at least three different glycerol dehydrogenases were formed under different physiological conditions: the NAD-dependent enzyme described above, a constitutive NADP-dependent enzyme and a -glyceraldehyde-specific enzyme induced on -galacturonate. The glycerol kinase mutant showed impaired growth on -galacturonate.

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1990-07-01
2024-04-26
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References

  1. Adler L., Blomberg A., Nilsson A. 1985; Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii . Journal of Bacteriology 162:300–306
    [Google Scholar]
  2. Baliga B.S., Bhatnagar G.M., Jagannathan V. 1962; Triphosphopyridine nucleotide-specific glycerol dehydrogenase from Aspergillus niger . Biochimica et Biophysica Acta 58:384–385
    [Google Scholar]
  3. Bensadoun A., Weinstein D. 1976; Assay of proteins in the presence of interfering materials. Analytical Biochemistry 70:241–250
    [Google Scholar]
  4. Beever R.E., Laracy E.P. 1986; Osmotic adjustment in the filamentous fungus Aspergillus nidulans . Journal of Bacteriology 168:1358–1365
    [Google Scholar]
  5. Bos C.J. 1987; Induction and isolation of mutants in fungi at low mutagen doses. Current Genetics 12:471–474
    [Google Scholar]
  6. Bos C.J., Debets A.J.M., Swart K., Huybers A., Kobus G., Slakhorst S.M. 1988; Genetic analysis of Aspergillus niger and the construction of master strains for assignment of genes to linkage groups in Aspergillus niger . Current Genetics 14:437–443
    [Google Scholar]
  7. Brown A.D. 1976; Microbial water stress. Bacteriological Reviews 40:803–846
    [Google Scholar]
  8. Brown A.D. 1978; Compatible solutes and extreme water stress in eukaryotic micro-organisms. Advances in Microbial Physiology 17:181–242
    [Google Scholar]
  9. Courtright J.B. 1975a; Intracellular localization and properties of glycerokinase and glycerolphosphate dehydrogenase in Neurospora crassa . Archives of Biochemistry and Biophysics 167:34–44
    [Google Scholar]
  10. Courtright J.B. 1975b; Differential rates of synthesis of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa during induction. Archives of Biochemistry and Biophysics 167:21–33
    [Google Scholar]
  11. Dijkema C., Kester H.C.M., Visser J. 1985; Carbon-13 NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans . Proceedings of the National Academy of Sciences of the United States of America 82:14–18
    [Google Scholar]
  12. Gancedo C., Gancedo J.M., Sols A. 1968; Glycerol metabolism in yeasts. Pathways of utilization and production. European Journal of Biochemistry 5:165–172
    [Google Scholar]
  13. Gancedo C., Llobell A., Ribas J.C., Luchi F. 1986; Isolation and characterization of mutants from Saccharomyces pombe defective in glycerol catabolism. European Journal of Biochemistry 159:171–174
    [Google Scholar]
  14. Hocking A.D., Norton R.S. 1983; Natural-abundance 13C nuclear magnetic resonance studies on the internal solutes of xerophilic fungi. Journal of General Microbiology 129:2915–2925
    [Google Scholar]
  15. Holm K., Nilheden E., Kolmark H.G. 1976; Genetic and enzymatic analysis of a glycerol kinase deficient mutant in Neurospora crassa . Molecular and General Genetics 144:11–15
    [Google Scholar]
  16. Itzhaki R.F., Gill O.M. 1964; A microbiuret method for estimating proteins. Analytical Biochemistry 9:401–410
    [Google Scholar]
  17. Kong Y.-C., May J.W., Marshall J.H. 1985; Glycerol oxidation and triose reduction by pyridine nucleotide-linked enzymes in the fission yeast Schizosaccharomyces pombe . Journal of General Microbiology 131:1571–1579
    [Google Scholar]
  18. De Koning W., Harder W., Dijkhuizen L. 1987; Glycerol metabolism in the methylotrophic yeast Hansenula polymorpha: phosphorylation as the initial step. Archives of Microbiology 148:314–320
    [Google Scholar]
  19. Legisa M., Mattey M. 1986; Glycerol as an initiator of citric acid accumulation in Aspergillus niger . Enzyme and Microbial Technology 8:258–259
    [Google Scholar]
  20. Lin E.C.C. 1976; Glycerol dissimilation and its regulation in bacteria. Annual Review of Microbiology 30:535–578
    [Google Scholar]
  21. Lin E.C.C. 1977; Glycerol utilization and its regulation in mammals. Annual Review of Biochemistry 46:765–795
    [Google Scholar]
  22. May J.W., Sloan J. 1981; Glycerol utilization by Schizosaccharomyces pombe: dehydrogenation as the initial step. Journal of General Microbiology 123:183–185
    [Google Scholar]
  23. May J.W., Marshall J.H., Sloan J. 1982; Glycerol utilization by Schizosaccharomyces pombe: phosphorylation of dihydroxyace-tone by a specific kinase as the second step. Journal of General Microbiology 128:1763–1766
    [Google Scholar]
  24. Mcgregor W.G., Phillips J., Suelter C.H. 1974; Purification and kinetic characterization of a monovalent cation-activated glycerol dehydrogenase from Aerobacter aerogenes . Journal of Biological Chemistry 249:3132–3139
    [Google Scholar]
  25. Payton M.A. 1978 A genetic study of sugar metabolism and transport in Aspergillus nidulans. PhD thesis University of Leicester, UK:
    [Google Scholar]
  26. Pontecorvo G., Roper J.A., Hemmons L.M., MacDonald K.D., Bufton A.W.J. 1953; The genetics of Aspergillus nidulans . Advances in Genetics 5:141–239
    [Google Scholar]
  27. Röhr M., Kubicek C.P., Zehentgruber O., Orthofer R. 1987; Accumulation and partial re-consumption of polyols during citric acid fermentation by Aspergillus niger . Applied Microbiology and Biotechnology 27:235–239
    [Google Scholar]
  28. Schuurink R., Busink R., Hondmann D.H.A., Witteveen C.F.B., Visser J. 1990; Purification and properties of NADP+-dependent glycerol dehydrogenase from Aspergillus nidulans and A. niger . Journal of General Microbiology 136:1043–1050
    [Google Scholar]
  29. St Martin E.J., Freedberg W.B., Lin E.C.C. 1975; Kinase replacement by a dehydrogenase for Escherichia coli glycerol utilization. Journal of Bacteriology 131:1026–1028
    [Google Scholar]
  30. Uitzetter J.H.A., Bos C.J., Visser J. 1986; Characterization of Aspergillus nidulans mutants in carbon metabolism isolated after d-galacturonate enrichment. Journal of General Microbiology 132:1167–1172
    [Google Scholar]
  31. Van Laere A.J., Hulsmans E. 1987; Water potential, glycerol synthesis, and water content of germinating Phycomyces spores. Archives of Microbiology 147:257–262
    [Google Scholar]
  32. Visser J., Van Rooijen R., Dijkema C., Swart K., Sealy-Lewis H.M. 1988; Glycerol uptake mutants of the hyphal fungus Aspergillus nidulans . Journal of General Microbiology 134:655–659
    [Google Scholar]
  33. Viswanath-Reddy M., Pyle J.E., Howe H.B. 1978; Purification and properties of NADP+-linked glycerol dehydrogenase from Neurospora crassa . Journal of General Microbiology 10 7:289–296
    [Google Scholar]
  34. Weibel K.E., Mor J.R., Fiechter A. 1974; Rapid sampling of yeast cells and automated assays of adenylate, citrate, pyruvate and glucose-6-phosphate pools. Analytical Biochemistry 58:208–216
    [Google Scholar]
  35. Witteveen C.F.B., Busink R., Van De Vondervoort P., Dijkema C., Swart K., Visser J. 1989; L-Arabinose and d-xylose catabolism in Aspergillus niger . Journal of General Microbiology 135:2163–2171
    [Google Scholar]
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