1887

Microbiology Society logo

Volume 136, Issue 7

The occurrence and function of -type cytochromes in the aerobic respiratory chain of NCTC 1937 grown under O-sufficient and O-limited conditions Free

Abstract

Intact cells of (NCTC 1937) after growth under O-limited conditions in batch culture exhibit, in addition to - and -type cytochromes, an unusual pigment absorbing variably at 588 to 594 nm in reduced oxidized difference spectra at room temperature. CO difference spectra suggest ligand binding by this pigment and also reveal a prominent 448 nm absorption minimum and CO-binding - and/or -type cytochromes. Although the 588 to 594 nm-absorbing component is reminiscent of ‘cytochrome ’, claimed to be a terminal oxidase in some bacteria, O-limited cells of contain no detectable haem A. In contrast, cells grown under O-sufficient conditions exhibit a membrane-bound cytochrome and contain haem A. Low-temperature photodissociation studies of O-sufficient cells show cytochrome to be functional in CO and O binding and suggest that is the terminal oxidase of a cytochrome - and -containing aerobic respiratory chain. Analogous studies of O-limited cells reveal a component absorbing, in its unliganded state, at 448 nm. Exposure of such CO-liganded cells to white actinic light is followed by oxidation of - and/or -type cytochromes and, although the functional oxidase has not been identified, we conclude that does not utilize a cytochrome oxidase of the type.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1990
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1167
1990-07-01
2023-06-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1167.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1167&mimeType=html&fmt=ahah

References

  1. Castor L.N., Chance B. 1955; Photochemical action spectra of carbon monoxide-inhibited respiration. Journal of Biological Chemistry 217:453–465
     [Google Scholar]
  2. Castor L.N., Chance B. 1959; Photochemical determinations of the oxidases of bacteria. Journal of Biological Chemistry 234:1587–1597
     [Google Scholar]
  3. Chance B., Saronio L., Leigh J.S. 1975; Functional intermediates in the reaction of membrane-bound cytochrome oxidase with oxygen. Journal of Biological Chemistry 250:9226–9237
     [Google Scholar]
  4. Davis G.H.G., Park R.W.A. 1962; A taxonomic study of certain bacteria currently classified as Vibrio species. Journal of General Microbiology 27:101–119
     [Google Scholar]
  5. De Maio R.N., Webster D.A., Chance B. 1983; Spectral evidence for the existence of a second cytochrome o in whole cells of Vitreoscilla. . Journal of Biological Chemistry 258:13768–13771
     [Google Scholar]
  6. Ereckinska M., Chance B. 1972; Studies on the electron transport chain at subzero temperatures: electron transport at site III. Archives of Biochemistry and Biophysics 151:304–315
     [Google Scholar]
  7. Fuhrhop J.H., Smith K.M. 1975; Laboratory Methods. In Porphyrins and Metalloporphyrins pp 757–869 Smith K. M. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  8. Fukomori Y., Fujiwara T., Okada-Takahashi Y., Mukohata Y., Yamanaka T. 1985; Purification and properties of a peroxidase from Halobacterium halobium L-33. Journal of Biochemistry 98:1055–1061
     [Google Scholar]
  9. Keilin D. 1966 The History of Cell Respiration and Cytochrome Cambridge: Cambridge University Press;
     [Google Scholar]
  10. Lorence R.M., Koland J.G., Gennis R.B. 1986; Coulometric and spectroscopic analysis of the purified cytochrome d complex of Escherichia coli: evidence for the identification of‘cytochrome a1’ as cytochrome b595. . Biochemistry 25:2314–2321
     [Google Scholar]
  11. Ludwig B. 1987; Cytochrome c oxidase in prokaryotes. FEMS Microbiology Reviews 46:41–56
     [Google Scholar]
  12. Markwell M.A.K., Haas S.M., Bieber L.L., Tolbert N.F. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87:206–210
     [Google Scholar]
  13. Matsushita K., Yamada M., Shinagawa E., Adachi O., Ameyama M. 1983; Membrane-bound respiratory chain of Pseudomonas aeruginosa grown aerobically. A KCN-insensitive alternate oxidase chain and its energetics. Journal of Biochemistry 93:1137–1144
     [Google Scholar]
  14. Poole R.K. 1977; The influence of growth substrate and capacity for oxidative phosphorylation on respiratory oscillations in synchronous cultures of Escherichia coli K12. Journal of General Microbiology 99:369–377
     [Google Scholar]
  15. Poole R.K. 1983; Bacterial cytochrome oxidases. A structurally and functionally diverse group of electron-transfer proteins. Biochimica et Biophysica Acta 726:205–243
     [Google Scholar]
  16. Poole R.K. 1988; Bacterial cytochrome oxidases. In Bacterial Energy Transduction pp 231–291 Anthony C. Edited by London: Academic Press;
     [Google Scholar]
  17. Poole R.K., Haddock B.A. 1974; Energy linked reduction of nicotinamide-adenine dinucleotide in membranes derived from normal and various respiratory-deficient mutant strains of Escherichia coli K12. Biochemical Journal 144:77–85
     [Google Scholar]
  18. Poole R.K., Williams H.D. 1987; Proposal that the function of the membrane-bound cytochrome a1-like haemoprotein (‘cytochrome b595’) in Escherichia coli is direct electron donation to cytochrome d. . FEBS Letters 217:49–52
     [Google Scholar]
  19. Poole R.K., Lloyd D., Chance B. 1979a; The reaction of cytochrome oxidase with oxygen in the fission yeast Schizosaccharo- myces pombe 972h−. Biochemical Journal 184:555–563
     [Google Scholar]
  20. Poole R.K., Waring A.J., Chance B. 1979b; The reaction of cytochrome o in Escherichia coli with oxygen. Low temperature kinetics and spectral studies. Biochemical Journal 184:379–389
     [Google Scholar]
  21. Poole R.K., Sivaram A., Salmon I., Chance B. 1982; Photolysis at very low temperatures of CO-liganded cytochrome oxidase (cytochrome d) in oxygen-limited Escherichia coli. . FEBS Letters 141:237–241
     [Google Scholar]
  22. Poole R.K., Baines B.S., Curtis S.J., Williams H.D., Wood P.M. 1984; Haemoprotein b-590 (Escherichia coli) ; redesignation of a bacterial ‘cytochrome a1’. . Journal of General Microbiology 130:3055–3058
     [Google Scholar]
  23. Poole R.K., Baines B.S., Williams H.D. 1985; Sensor sensationalism? Alternative views on the nature and role of ‘cytochrome a1’ in bacteria. Microbiological Sciences 2:21–24
     [Google Scholar]
  24. Poole R.K., Baines B.S., Appleby C.A. 1986; Haemoprotein b-590 (Escherichia coli), a reducible catalase and peroxidase: evidence for its close relationship to hydroperoxidase I and a ‘cytochrome a1b’ preparation. Journal of General Microbiology 132:1525–1539
     [Google Scholar]
  25. Sone N., Naqui A., Kumar C., Chance B. 1984; Reaction of caa3-type terminal oxidase from the thermophilic bacterium PS3 with oxygen and carbon monoxide at low temperatures. Biochemical Journal 221:529–533
     [Google Scholar]
  26. Spicher G. 1974; Cytochrome absorption spectra of bacteria as aid for solving taxonomic problems. 2nd Report: the redox and carbon monoxide difference spectra of bacterial species of the families Enterobacteriaceae and Pasteurellaceae, and of the genera Aero-monas, Xanthomonas, Vibrio, Acinetobacter, Alcaligenes, and Achro-mobacter. . Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene, Series A 226:541–553
     [Google Scholar]
  27. Stanier R.Y., Palleroni N.J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
     [Google Scholar]
  28. Wikström M., Krab K., Saraste M. 1981 Cytochrome oxidase. A Synthesis London: Academic Press;
     [Google Scholar]
  29. Williams H.D., Poole R.K. 1986; Low temperature spectroscopic studies of cytochromes a1 and d in Acetobacter pasteurianus. . Biochemical Society Transactions 14:1215
     [Google Scholar]
  30. Williams H.D., Poole R.K. 1987; The cytochromes of Acetobacter pasteurianus NCIB 6428. Evidence of a role for a cytochrome a1-like haemoprotein in electron-transfer to cytochrome oxidase d. . Journal of General Microbiology 133:2461–2472
     [Google Scholar]
  31. Williams H.D., Poole R.K. 1988; Cytochromes of Acetobacter pasteurianus NCIB 6428: reaction with oxygen of cytochrome o in cells, membranes and non-sedimentable subcellular fractions. Current Microbiology 16:277–280
     [Google Scholar]
  32. Wood P.M. 1984; Bacterial proteins with CO-binding B or c type haem: functions and absorption spectroscopy. Biochimica et Biophysica Acta 768:293–317
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1167
Loading
The occurrence and function of a-type cytochromes in the aerobic respiratory chain of Comamonas percolans NCTC 1937 grown under O2-sufficient and O2-limited conditions
Microbiology 136, 1167 (1990); https://doi.org/10.1099/00221287-136-7-1167
/content/journal/micro/10.1099/00221287-136-7-1167
/content/journal/micro/10.1099/00221287-136-7-1167
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error