1887

Microbiology Society logo

Volume 136, Issue 6

Isolation of a species able to grow on malonate under strictly anaerobic conditions Free

Abstract

An anaerobic enrichment from lake mud yielded a pure culture of a facultatively anaerobic bacterium able to grow on malonate under strictly anaerobic conditions. Strain 16mall was identified as a member of the family , and assigned to the genus on the basis of morphological, metabolic and biochemical characteristics. Malonate was fermented under strictly anaerobic (sulphide-reduced) conditions to acetate and CO concomitant with growth. A maximum growth rate of 1·88 generations h (μ = 1·30 h) was measured. The dry weight yield of cells from malonate was estimated at 2·5 g mol. Yeast extract was required for growth on malonate: other additives, or a vitamin solution, could not replace this requirement. Other dicarboxylic acids were not degraded in the absence or presence of malonate. Malonate was degraded under anaerobic, but not aerobic conditions. Malonate-decarboxylating activity was inducible by malonate under both anaerobic and aerobic conditions, and was not expressed in glucose- or citrate-grown anaerobic cultures. Monensin had no effect on malonate degradation, while 2,4-dinitrophenol decreased the rate of malonate degradation. This, with the lack of a sodium requirement for anaerobic growth on malonate, suggested that ATP generation may not be mediated by a sodium-pumping mechanism.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-6-1037
1990-06-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/6/mic-136-6-1037.html?itemId=/content/journal/micro/10.1099/00221287-136-6-1037&mimeType=html&fmt=ahah

References

  1. Badziong W., Thauer 1980. 1980; Vectorial electron transport in Desulfovibrio vulgaris (Marburg) growing on hydrogen plus sulfate as sole energy source. Archives of Microbiology 125:167–174
     [Google Scholar]
  2. Brenner D.J. 1984; Enterobacteriaceae. In Bergey’s Manual of Systematic Bacteriology 1 pp 408–420 Krieg N. R., Holt J. G. Edited by Baltimore: Williams & Wilkins;
     [Google Scholar]
  3. Breznak J.A., Switzer J.M., Seitz H.J. 1988; Sporomusa termitida sp. nov., an H2/CO2-utilizing acetogen isolated from termites. Archives of Microbiology 150:282–288
     [Google Scholar]
  4. Cerny G. 1978; Studies on the aminopeptidase test for the distinction of Gram-negative from Gram-positive bacteria. European Journal of Applied Microbiology and Biotechnology 5:113–122
     [Google Scholar]
  5. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. Journal of Microbiological Methods 4:33–36
     [Google Scholar]
  6. Dehning I., Schink B. 1989; Malonomonas rubra gen. nov., sp. nov., a microaerotolerant anaerobic bacterium growing by decarboxylation of malonate. Archives of Microbiology 151:427–433
     [Google Scholar]
  7. Dehning I., Stieb M., Schink B. 1989; Sporomusa malonica sp. nov., a homoacetogenic bacterium growing by decarboxylation of malonate or succinate. Archives of Microbiology 151:421–426
     [Google Scholar]
  8. Dimroth P. 1982a; The role of biotin and sodium in the decarboxylation of oxaloacetate by the membrane-bound oxaloace- tate decarboxylase from Klebsiella aerogenes. . European Journal of Biochemistry 121:435–441
     [Google Scholar]
  9. Dimroth P. 1982b; The generation of an electrochemical gradient of sodium ions upon decarboxylation of oxaloacetate by the membrane- bound and Na+-activated oxaloacetate decarboxylase from Klebsiella aerogenes. . European Journal of Biochemistry 121:443–449
     [Google Scholar]
  10. Doetsch R.N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp 21–33 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Edited by Washington, DC: American Society for Microbiology;
     [Google Scholar]
  11. Hilpert W., Schink B., Dimroth P. 1984; Life by a new decarboxylation-dependent energy conservation mechanism with Na+ as coupling ion. EMBO Journal 3:1665–1670
     [Google Scholar]
  12. Krieg N.R., Holt J.G. 1984 Bergey’s Manual of Systematic Bacteriology 1 Baltimore: 3Williams & Wilkins;
     [Google Scholar]
  13. O’Brien R.W., Stern J.R. 1969; Requirement for sodium in the anaerobic growth of Aerobacter aerogenes on citrate. Journal of Bacteriology 98:388–393
     [Google Scholar]
  14. Owen R.J., Lapage S.P. 1976; The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic application. Journal of Applied Bacteriology 41:335–340
     [Google Scholar]
  15. Patel B.K.C., Morgan H.W., Daniel R.M. 1985a; A simple and efficient method of preparing and dispensing anaerobic media. Biotechnology tetters 7:277–278
     [Google Scholar]
  16. Patel B.K.C., Morgan H.W., Daniel R.M. 1985b; Fervidobacterium nodosum gen. nov. and spec, nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Archives of Microbiology 141:63–69
     [Google Scholar]
  17. Patel B.K.C., Monk C., Littleworth H., Morgan H.W., Daniel R.M. 1987; Clostridium fervidus sp. nov., a new chemoorganotrophic acetogenic thermophile. International Journal of Systematic Bacteriology 37:123–126
     [Google Scholar]
  18. Pfennig N. 1978; Rhodocyclus purpureus gen. nov. sp. nov., a ringshaped, vitamin B12-requiring member of the family Rhodospirilla- ceae. . International Journal of Systematic Bacteriology 28:283–288
     [Google Scholar]
  19. Sakazaki R. 1984; Citrobacter. In Bergey’s Manual of Systematic Bacteriology 1 pp 458–461 Krieg N. R., Holt J. G. Edited by Baltimore: Williams & Wilkins;
     [Google Scholar]
  20. Schink B., Pfennig N. 1982; Propionigenium modestum gen nov. sp. nov., a new strictly anaerobic, nonsporing bacterium growing on succinate. Archives of Microbiology 133:209–216
     [Google Scholar]
  21. Smibert R.M., Krieg N.R. 1981; General characterization. In Manual of Methods for General Bacteriology pp 409–443 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Edited by Washington, DC: American Society for Microbiology;
     [Google Scholar]
  22. Stern J.R. 1967; Oxaloacetate decarboxylase from Aerobacter aerogenes. I. Inhibition by avidin and requirement for sodium ion. Biochemistry 6:3545–3551
     [Google Scholar]
  23. Stouthamer A.H. 1979; The search for correlation between theoretical and experimental growth yields. In International Review of Biochemistry 21 Microbial Biochemistry pp 1–47 Quayle J. R. Edited by Baltimore: University Park Press;
     [Google Scholar]
  24. Trüper H.G., Pfennig N. 1981; Characterization and identification of the anoxygenic phototrophic bacteria. In The Prokaryotes 1 pp 299–312 Starr M. P., Stolp H., Truper H. G., Balows A., Schlegel H. G. Edited by Berlin: Springer-Verlag;
     [Google Scholar]
  25. Tschech A., Pfennig N. 1984; Growth yield increase linked to caffeate reduction in Acetobacterium woodii. . Archives of Microbiology 137:163–167
     [Google Scholar]
/content/journal/micro/10.1099/00221287-136-6-1037
Loading
Isolation of a Citrobacter species able to grow on malonate under strictly anaerobic conditions
Microbiology 136, 1037 (1990); https://doi.org/10.1099/00221287-136-6-1037
/content/journal/micro/10.1099/00221287-136-6-1037
/content/journal/micro/10.1099/00221287-136-6-1037
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error