Summary: belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the serovar , using the vector pUC13. We have isolated DNA fragments containing the genetic information required to complement strains of with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented also complemented , a 15 kb fragment complementing could not complement other mutations. The origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error