Summary: belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the serovar , using the vector pUC13. We have isolated DNA fragments containing the genetic information required to complement strains of with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented also complemented , a 15 kb fragment complementing could not complement other mutations. The origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species


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