1887

Abstract

Summary: belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the serovar , using the vector pUC13. We have isolated DNA fragments containing the genetic information required to complement strains of with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented also complemented , a 15 kb fragment complementing could not complement other mutations. The origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species

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/content/journal/micro/10.1099/00221287-136-4-651
1990-04-01
2019-11-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-4-651
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