RT Journal Article SR Electronic(1) A1 Richaud, Catherine A1 Margarita, Danielle A1 Baranton, Guy A1 Girons, Isabelle SaintYR 1990 T1 Cloning of genes required for amino acid biosynthesis from Leptospira interrogans serovar icterohaemorrhagiae JF Microbiology, VO 136 IS 4 SP 651 OP 656 DO https://doi.org/10.1099/00221287-136-4-651 PB Microbiology Society, SN 1465-2080, AB Leptospira interrogans belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the serovar icterohaemorrhagiae, using the Escherichia coli vector pUC13. We have isolated Leptospira DNA fragments containing the genetic information required to complement strains of E. coli with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented proA also complemented proB, a 15 kb fragment complementing leuB could not complement other leu mutations. The L. interrogans origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species L. biflexa. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-4-651