SUMMARY: Mitochondrial DNA (mt DNA) from a patulin producer, (synonym ), was 27.8 kb ± 0.6 kb in size by electron microscopy and 27.2 kb by agarose gel electrophoresis. Restriction endonuclease maps for nine restriction enzymes were constructed, and eleven fragments which covered the total range of the mt DNA were cloned into the plasmid vector pUC19. Southern analysis of the native genomes of and with six of the cloned fragments as probes indicated similar genome arrangements as well as similar restriction maps. Both the large and small rRNA genes of and P. chrysogenum were located on these restriction maps using Southern hybridization, and the result also supported the similar arrangement. Agarose/formaldehyde gel electrophoresis indicated that the small rRNA was 1.5 kb in size in both species; but, surprisingly, the large rRNA was 4.2 kb in size for and 3.5 kb for . These sizes were, respectively, 1.1 kb and 0.4 kb larger than those from the very closely related .


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