1887

Abstract

Mitochondrial DNA (mt DNA) from a patulin producer, (synonym ), was 27·8 kb ± 0·6 kb in size by electron microscopy and 27.2 kb by agarose gel electrophoresis. Restriction endonuclease maps for nine restriction enzymes were constructed, and eleven fragments which covered the total range of the mt DNA were cloned into the plasmid vector pUC19. Southern analysis of the native genomes of and with six of the cloned fragments as probes indicated similar genome arrangements as well as similar restriction maps. Both the large and small rRNA genes of and P. chrysogenum were located on these restriction maps using Southern hybridization, and the result also supported the similar arrangement. Agarose/formaldehyde gel electrophoresis indicated that the small rRNA was 1·5 kb in size in both species; but, surprisingly, the large rRNA was 4·2 kb in size for and 3·5 kb for . These sizes were, respectively, 1·1 kb and 0·4 kb larger than those from the very closely related .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-3-535
1990-03-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/3/mic-136-3-535.html?itemId=/content/journal/micro/10.1099/00221287-136-3-535&mimeType=html&fmt=ahah

References

  1. Botstein D., Falco S. C., Stewart S. E., Brennan M., Scherer S., Stinchcomb D. T., Struhl K., Davis R. W. 1979; Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24
    [Google Scholar]
  2. Cummings D. J., Belcour L., Grandchamp C. 1979; Mitochondrial DNA from Podospora anserina . Molecular and General Genetics 171:229–238
    [Google Scholar]
  3. Dujon B. 1980; Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci. Cell 20:185–197
    [Google Scholar]
  4. Dyson N. J., Brown T. A., Waring R. B., Davies R. W. 1989; The mitochondrial ribosomal RNA molecules of Aspergillus nidulans . Gene 75:109–118
    [Google Scholar]
  5. Falconet D., Delorme S., Lejeune B., Sévignac M., Delcher E., Bazetoux S., Quétier F. 1985; Wheat mitochondrial 26S ribosomal RNA gene has no intron and is present in multiple copies arising by recombination. Current Genetics 9:169–174
    [Google Scholar]
  6. Garriga G., Lambowitz A. M. 1984; RNA splicing in Neurospora mitochondria: self-splicing of a mitochondrial intron in vitro . Cell 38:631–641
    [Google Scholar]
  7. Garriga G., Bertrand H., Lambowitz A. M. 1984; RNA splicing in Neurospora mitochondria: nuclear mutants defective in both splicing and 3′ end synthesis of the large rRNA. Cell 36:623–634
    [Google Scholar]
  8. Georgiev O. I., Nikolaev N., Hadjiolov A. A., Skryabin K. G., Zakharyev V. M., Bayev A. A. 1981; The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25S rRNA gene from Saccharomyces cerevisiae . Nucleic Acids Research 96953–6958
    [Google Scholar]
  9. Green M. R., Grimmm F., Goewert R. R., Collins R. A., Cole M. D., Lambowita Z., Heckman J., Yin S., Rajbhandary U. 1981; Transcripts and processing patterns for the ribosomal RNA and transfer RNA region of Neurospora crassa mitochondria1 DNA. Journal of Biologica1 Chemistry 2562027–2043
    [Google Scholar]
  10. Grossman L. I., Hudspeth M.E.S. 1985; Fungal niitochondrial genomes. In Gene Manipulations in Fungi pp. Bennett J. W., Lasure L. L. Edited by London: Academic Press;
    [Google Scholar]
  11. Hoffman C.S, Winston F. 1987; A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli . Gene 57:267–272
    [Google Scholar]
  12. Ito H., Fukuda Y., Murata K., Kimura A. 1983; Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology 153:163–168
    [Google Scholar]
  13. Köchel H. G., Küntzel H. 1981; Nucleotide sequence of the Aspergillus nidulans mitochondrial gene coding for the small ribosomal subunit RNA: homology to E. coli 16S rRNA. Nucleic Acids Research 9:5689–5696
    [Google Scholar]
  14. Köchel H. G., Küntzel H. 1982; Mitochondrial L-rRNA from Aspergillus nidulans: potential secondary structure and evolution. Nucleic Acids Research 10:4795–4801
    [Google Scholar]
  15. Kozlowski M., Stepień P. P. 1982; Restriction enzyme analysis of mitochondrial DNA of members of the genus Aspergillus as an aid in taxonomy. Journal of General Microbiology 128:471–476
    [Google Scholar]
  16. Lambowitz A. M. 1989; Infectious introns. Cell 56:323–326
    [Google Scholar]
  17. Lang D., Mitani M. 1970; Simplified quantitative electron microscopy of biopolymers. Biopolymers 9:373–379
    [Google Scholar]
  18. Leaver C. J., Gray M. W. 1982; Mitochondrial genome organization and expression in higher plants. Annual Review of Plant Physiology 33:373–402
    [Google Scholar]
  19. Lehrach H., Diamond D., Wozney J. M., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
    [Google Scholar]
  20. Li M., Tzagoloff A., Underbrick-Lyon K., Martin N. C. 1982; Identification cf the paromomycin-resistance mutation in the 15S rRNA gene of yeast mitochondria. Journal of Biological Chemistry 257:5921–5928
    [Google Scholar]
  21. Mahler H. R. 1983; The exon:intron structure of some mitochondrial genes and its relation to mitochondrial evolution. International Review of Cytology 821–98
    [Google Scholar]
  22. Maniatis T., Fritsch E.F. 1982 Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Mannella C. A., Collins R. A., Green M. R., Lambowitz A. M. 1979; Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa . Proceedings of the National Academy of Sciences of the United States of America 76:2635–2639
    [Google Scholar]
  24. Mantle P. G. 1987; Secondary metabolites of Penicillium and Acremonium . In Penicillium and Acremonium (Biotechnology Handbook 1 pp. 161–243 Peberdy J. F. Edited by New York: Plenum Press;
    [Google Scholar]
  25. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  26. Merten S., Synenki R. M., Jocker J., Christianson T., Rabinowitz M. 1980; Processing of precursors of 21S ribosomal RNA from yeast mitochondria. Proceedings of the National Academy of Sciences of the United States of America 77:1417–1421
    [Google Scholar]
  27. Netzker R., Kochel H. G., Basak N., Küntzel H. 1982; Nucleotide sequence of Aspergillus nidulans mitochondrial genes coding for ATPase subunit 6,cytochrome oxidase subunit 3 seven unidentified proteins, four tRNAs and L-rRNA. Nucleic Acids Research 10:4783–4794
    [Google Scholar]
  28. Picknett T. M., Saunders G., Ford P., Holt G. 1987; Development of a gene transfer system for Penicillium chrysogenum . Current Genetics 12:449–455
    [Google Scholar]
  29. Rubtsov P. M., Musakhanov M. M., Zakharyev V. M., Krayev A. S., Skryabin K. G., Bayev A. A. 1980; The structure of the yeast ribosomal RNA genes. 1. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae . Nucleic Acids Research 8:5779–5794
    [Google Scholar]
  30. Saunders G., Holt G. 1987; Genetics of the penicillia. In Penicillium and Acremonium (Biotechnology Handbook 1 pp. Peberdy J. F. Edited by New York: Plenum Press;
    [Google Scholar]
  31. Saunders G., Rogers M. E., Adlard M. W., Holt G. 1984; Chromatographic resolution of nucleic acids extracted from Penicillium chrysogenum . Molecular and General Genetics 194:343–345
    [Google Scholar]
  32. Sekiguchi J., Gaucher G. M. 1977; Conidiogenesis and secondary metabolism in Penicillium urticae . Applied and Environmen tal Microbiology 33:147–158
    [Google Scholar]
  33. Sekiguchi J, Gaucher G. M. 1978; Identification of phyllostine as an intermediate of the patulin pathway in Penicillium urticae . Biochemistry 17:1785–1791
    [Google Scholar]
  34. Sekiguchi J., Shimamoto T., Yamada Y., Gaucher G. M. 1983; Patulin biosynthesis: enzymatic and nonenzymatic transformations of the mycotoxin (E)-ascladiol. Applied and Environmental Microbiology 45:1939–1942
    [Google Scholar]
  35. Sharp P. A., Sugden B., Sambrock J. 1973; Detection of two restriction endonuclease activities in Haemophilus parumtiuenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12:3055–3063
    [Google Scholar]
  36. Silhavy T. J., Berman M. L., Enquist L. 1984 Experiments with Gene Fusions. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Smith T. M., Saunders G., Stacey L. M., Holt G. 1984; Restriction endonuclease map of mitochondrial DNA from Penicillium chrysogenum . Journal of Biotechnology 1:37–46
    [Google Scholar]
  38. Sor F., Fukuuchara F. 1983; Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria. Nucleic Acids Research 11:339–348
    [Google Scholar]
  39. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated bv gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  40. Stahl U., Leitner E., Esser K. 1987; Transformation of Penicillium chrysogenum by a vector containing a mitochondrial origin of replication. Applied Microbe',log v and Biotechnology 26:237–241
    [Google Scholar]
  41. Stepien P. P., Bernard r., Cooke H. J., Kcntzei H. 1978; Restriction endonuclease cleavage map: cf mitochondrial DNA from Aspergillus nidulans . Nucleic Acids Research 5317–330
    [Google Scholar]
  42. Strlhi K., Stjnchccmb D.T, Scherker S., Davis R. W. 1979; High frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proceedings of the National Academy of Sciences of the United Slates of America 76:1035–1039
    [Google Scholar]
  43. Tudynski P., Esser K. 1985; Mitochondrial DNA for gene cloning in eukaryotes. In Gene Manipulations in Fungi pp. 403–416 Bennett J. W., Lasure L.L. Edited by London: Academic Press;
    [Google Scholar]
  44. Wright R. M., Lapikg J. L., Horrum M. A., Cummings D. J. 1987; MAChrndiiai DNA from Fodospora anserina. III. Cloning, physical mapping, and localization of the ribosomal RNA genes. Molecular and General Genetics 18556–64
    [Google Scholar]
  45. Yanisch-perron C, Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  46. Zamir L. O. 1980; The biosynthesis of patulin and penicillic acid. In The Biosynthesis of Mvcotoxins pp. 223–268 Steyn P. S. Edited by New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-3-535
Loading
/content/journal/micro/10.1099/00221287-136-3-535
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error